Functions of water channel in the growth and development of horticultural crops.

水道在园艺作物生长发育中的作用.

基本信息

批准号：
13460012
负责人：
SHIRATAKE Katsuhiro
金额：
$ 4.61万
依托单位：
Nagoya University, Graduation School of Bioagricultural Sciences
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

1, Water Channel in Fruit(1)Protein level of tonoplast water channel (TIP) of pear was high in young fruit, on the other hand, that of TIP and plasma membrane water channel (PIP) of grape berry was the almost constant during development. This suggests that water channel a play role in water regulation rather than fruit development.(2)Gene expression of TIP was high in young pear fruit, but that of PIP showed almost constant level during development. Gene expressions of TIP and PIP in fruit was especially higher than those in other organs, suggesting the importance of water channel in fruit.(3)Phosphorylation of PIP2 was determined using the antibody which could recognize only phosphorylated PIP2. PIP2 was de-phospholylared by salt and osmotic stresses and also in the fruit in day time.2, Water Channel in Radish(1)Protein and gene expressions of TIP and PIP were high in developing tissue or vascular tissue, such as central cylinder in young root and hypocotyl, and bundle sheath in petiole. This meets physiological function.(2)Water transport of the membrane from yeast expressed each TIP or PIP was measured. TIP and PIP2 transported water. On the other hand, PIPI did not transport water and it might play a role other than water transport.(3)By the treatment of osmotic stress or plant hormone to radish seedling, mRNA and protein levels of TIP and PIPL did not change, but those of PIP2 did. These results show that TIP and PIP are housekeeping type and PIP2 is environmentally inducible one.(4)The epidermis cells of hypocotyl and the parenchyma cells of taproot of radish were expressed water channels at high levels and their water transport activities were also high (300-500 mm/sec).

1、果实中的水通道 (1)梨的液泡膜水通道(TIP)蛋白水平在幼果期较高，而葡萄浆果的TIP和质膜水通道(PIP)蛋白水平在幼果期几乎恒定。发展。这表明水通道在水分调节中发挥作用，而不是在果实发育中发挥作用。(2)TIP基因在梨幼果中表达量较高，而PIP基因在发育过程中几乎保持恒定水平。尤其是果实中TIP和PIP的基因表达量明显高于其他器官，提示水通道在果实中的重要性。(3)利用只能识别磷酸化PIP2的抗体来测定PIP2的磷酸化。 PIP2在盐胁迫和渗透胁迫下发生去磷酸化，在白天的果实中也存在这种现象。2、萝卜中的水通道(1)蛋白质和TIP和PIP基因在发育组织或维管组织中表达量较高，如萝卜的中央圆柱体。幼根和下胚轴，叶柄内有束鞘。这符合生理功能。(2)测量表达各TIP或PIP的酵母的膜的水转运。 TIP 和 PIP2 输送水。另一方面，PIPI不运输水分，可能发挥着运输以外的作用。(3)通过渗透胁迫或植物激素处理萝卜幼苗，TIP和PIPL的mRNA和蛋白水平没有变化，但PIP2 的那些人做到了。这些结果表明，TIP和PIP是看家型的，PIP2是环境诱导型的。(4)萝卜下胚轴表皮细胞和主根薄壁组织细胞高水平表达水通道，其水分运输活性也很高(300 -500 毫米/秒）。