Molecular architecture and discontinuous evolution of plastid genomic machinery

质体基因组机器的分子结构和不连续进化

• 批准号: 13440234
• 负责人: SATO Naoki
• 金额: $ 9.6万
• 依托单位: Saitama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13440234/
• 关键词: chloroplast; plastid; endosymbiosis; nucleoid; RNA polymerase; sulfite reductase; cyanobacterium; 葉緑体核様体; DNA結合タンパク質; ゲノム進化

The following lines of evidence support our hypothesis on the discontinuous evolution of plastid genomic machinery.1. T7-type RNA polymerase (RPOT) is localized to only mitochondria. In angiosperms, the RPOT gene was duplicated to produce plastid-localyzed isozyme.2. PEND protein is a DNA-binding protein principally localized to developing plastids. In wounded tissues, this protein is also localized in the nucleus. This protein is only present in angiosperms, and therefore supposed to be newly added transcription factor in angiosperms.3. Sulfite reductase (SiR) is a major DNA-binding protein in the nucleoids of pea chloroplasts. The cDNA encoding SiR was isolated from pea. In the moss Physcomitrella patens, SiR was also a major component of plastid nucleoids. In red algal plastids and cyanobacteria, SiR was detected in the nucleoids, but was not the major component. This suggests that the major DNA-binding protein of nucleoid has been replaced during the evolution of plastids.4. Protein phosphorylation is involved in the regulation of transcription in plastid nucleoids.5. Computer-assisted analysis revealed a large number of plastid proteins that are supposed to originate from cyanobacterial endosymbionts.

以下证据支持我们对质体基因组机械不连续演变的假设1。 T7型RNA聚合酶（RPOT）仅定位于线粒体。在被子植物中，将RPOT基因复制以产生质体溶解同工酶2。 Pend蛋白是一种主要定位于发育质体的DNA结合蛋白。在受伤的组织中，该蛋白也位于细胞核中。该蛋白仅存在于被子植物中，因此被应为被子植物中新添加的转录因子。3。亚硫酸盐还原酶（SIR）是豌豆叶绿体核苷中的主要DNA结合蛋白。从豌豆中分离出编码SIR的cDNA。在Moss Physcomitrella patens中，SIR也是质体核苷的主要组成部分。在红色藻类质体和蓝细菌中，在核苷中检测到SIR，但不是主要成分。这表明在质体的演化过程中，核苷的主要DNA结合蛋白已被替换。4。蛋白质磷酸化参与质体核苷中转录的调节。5。计算机辅助分析显示，大量质子蛋白应该来自蓝细菌内共生体。

项目成果

佐藤直樹: "プラスチド核様体の分子構築・機能の多様性とゲノム装置の不連続進化仮説"生化学. 74. 27-37 (2002)

Naoki Sato：“质体核的分子结构和功能的多样性以及基因组装置不连续进化的假设”生物化学 74. 27-37 (2002)。

Y.Kabeya, K.Hashimoto, N.Sato: "Identification and characterization of two phage-type RNA polymerase cDNAs in the moss Physcomitrella patens"Plan Cell Physiol.. 43. 245-255 (2002)

Y.Kabeya、K.Hashimoto、N.Sato：“小立碗藓中两种噬菌体型 RNA 聚合酶 cDNA 的鉴定和表征”Plan Cell Physiol.. 43. 245-255 (2002)

N.Sato: "Comparative analysis of the genomes of cyanobacteria and plants"Genome Informatics. 13. 173-182 (2002)

N.Sato：“蓝藻和植物基因组的比较分析”基因组信息学。

K.Sekine, T.Hase, N.Sato: "Reversible DNA compaction by sulfite reductase regulates transcriptional activity of chloroplast nucleoids"J. Biol. Chem.. 277. 24349-24404 (2002)

K.Sekine、T.Hase、N.Sato：“亚硫酸还原酶可逆 DNA 压缩调节叶绿体核的转录活性”J.

K.Sekine, T.Hase, N.Sato: "Reversible DNA compaction by sulfite reductase regulates transcriptional activity of chloroplast nucleoids."J.Biol.Chem.. 277. 24399-24404 (2002)

K.Sekine、T.Hase、N.Sato：“亚硫酸盐还原酶可逆 DNA 压缩调节叶绿体核苷的转录活性。”J.Biol.Chem.. 277. 24399-24404 (2002)