Immunotherapy using cytotoxic T cell and peptide vaccine for pancreatic cancer

使用细胞毒性 T 细胞和肽疫苗治疗胰腺癌的免疫疗法

• 批准号: 12557104
• 负责人: OKA Masaaki
• 金额: $ 8.19万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12557104/
• 关键词: pancreatic cancer; CTL; cell therapy; cancer-related peptide; cancer vaccine therapy; dendritic cell; MUC1; ペプチド療法

(1) Fifteen patients (stage III : 4, stage Iva : 8, stage Ivb : 3) with pancreatic cancer were treated by immunotherapy using MUC1-CTL after curative resection. Only one patient had liver recurrence. One year and 2 year survival rate were 80% and 21.4%, respectively. This immunotherapy may prevent liver metastasis after surgery, but can not control local recurrence.(2) We performed phase I study of cancer peptide vaccine therapy for HLA-02 and -24 patients with unresectable pancreatic cancer. Three mg of peptides were administered with Freund's incomplete adjuvant subcutaneously. No sever adverse effect was observed. Seven of 10 patients had redness and edema in the injection sites. Three of 10 patients had NC and 7 had PD. One NC patient is still alive for 12 month. This vaccine therapy may be effective to control local lesion including regional lymph node metastasis.

（1）15例（第三阶段：4，IVA阶段：8，IVB：3阶段）患有胰腺癌，在治愈后，使用MUC1-CTL通过免疫疗法治疗胰腺癌。只有一名患者患有肝复发。一年和2年的生存率分别为80％和21.4％。这种免疫疗法可能会在手术后预防肝转移，但无法控制局部复发。（2）我们对HLA -02和-24患有无法切除的胰腺癌的患者进行了I期研究。皮下施用三毫克的肽用不完整的佐剂施用。未观察到严重的不良影响。 10例患者中有7名在注射部位有发红和水肿。 10例患者中有3名患有NC，有7例患有PD。一名NC患者还活着12个月。这种疫苗疗法可能有效控制包括区域淋巴结转移在内的局部病变。

项目成果

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Ohno R、Yamaguchi Y、Toge T、Kinouchi T、Kotake T、Shibata M、Kiyohara Y、Ikeda S、Fukui I、Gohchi A、Sugiyama Y、Saji S、Hazama S、Oka M、Ohnishi K、Ohhashi Y、Tsukagoshi S

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Yoshimura K、Hazama S、Iizuka N、Yoshino S、Yamamoto K、Muraguchi M、Ohmoto Y、Noma T、Oka M：“使用含有成熟白细胞介素 18 cDNA 的质粒载体转染的结肠癌细胞（结肠 26）进行成功的免疫基因治疗

Yoshino S, Tabata T, Hazama S, Iizuka N, Yamamoto K, Hirayama M, Tangoku A, Oka M: "Immunoregulatory effects of the antitumor polysaccharide lentinan on Th1/Th2 balance in patients with digestive cancers"Anticancer Research. 20. 4707-4711 (2000)

Yoshino S、Tabata T、Hazama S、Iizuka N、Yamamoto K、Hirayama M、Tangoku A、Oka M：“抗肿瘤多糖香菇多糖对消化道癌症患者 Th1/Th2 平衡的免疫调节作用”抗癌研究。

Suzuki N, Tanaka S, Hida N, Mine T, Yamamoto K, Oka M, Itoh K: "Detection of peptide-specific cytotoxic T lymphocyte precursors used for specific immunotherapy for pancreatic cancer"International Journal of Cancer. Mar 1 ; 98(1). 45-50 (2002)

Suzuki N、Tanaka S、Hida N、Mine T、Yamamoto K、Oka M、Itoh K：“用于胰腺癌特异性免疫治疗的肽特异性细胞毒性 T 淋巴细胞前体的检测”国际癌症杂志。

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Iizuka N、Oka M 等人：“使用寡核苷酸微阵列作为预测治愈性切除后肝细胞癌早期肝内复发的新方法”《柳叶刀》。