Study to prepare safe educational bacterial strains for medical education

研究制备用于医学教育的安全教育菌株

• 批准号: 12470062
• 负责人: EZAKI Takayuki
• 金额: $ 7.87万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470062/
• 关键词: Lebel 2; Lebel 3; Education; Biosafty; Pathogen; Medicine; 感染症; 病原細菌; 危険度レベル; 教育用菌株

Through this study, we constructed safe bacterial strains for educational purpose. Because level 3 and potent toxigenic level 2 bacterial pathogens are requested to use for medical education. However, most of university does not have appropriate facility for education. Class 3 facility is not prepared for student teaching room at most of medical universities. We collected information to decrease the level of pathogenicity for level 3 pathogens and selected candidate strains for educational purpose.Most candidate strains are knocked out their pathogenic factors. We propose to decrease biosafty level of these knocked out strains ofpathogenic factors. These strains should be widely distributed among researchers to evaluate their safety level in future

通过这项研究，我们构建了用于教育目的的安全菌株。因为医学教育中要求使用3级和2级强毒性细菌病原体。然而，大多数大学没有适当的教育设施。大多数医科大学没有为学生教学室准备三级设施。我们收集了降低3级病原体致病性水平的信息，并选择了用于教育目的的候选菌株。大多数候选菌株已被敲除其致病因子。我们建议降低这些被敲除的致病因子菌株的生物安全水平。这些菌株应在研究人员中广泛分发，以评估其未来的安全水平

项目成果

Takahashi, A., et al.: "Chloride secretion induced by thermostoble …"J. Med. Microbiol. 50. 870-878 (2001)

Takahashi, A., et al.：“耐热性引起的氯化物分泌……”J. Med Microbiol。 50. 870-878 (2001)

Yamamoto,S., Nakano,M., Terai,K., Nakata,K., Nair GB., Kurazono,H., Ogawa,O.: "The presence of the virulence island contains usp gene in uropathogenic Escherichia coli is associated with tract infection in an experimental mouse"J. Urol. 165. 1347-1351 (20

Yamamoto,S.、Nakano,M.、Terai,K.、Nakata,K.、Nair GB.、Kurazono,H.、Okawa,O.：“尿路致病性大肠杆菌中含有 usp 基因的毒力岛的存在与此相关

Nakano, M., et al.: "Structural and sequence diversity of the"FEMS Microbiol. Lett. 205. 71-76 (2001)

Nakano, M. 等人：“FEMS 微生物的结构和序列多样性”。

Licheng Zhao: "Vi Suppressed Wild Strain Salmonella Typhi Cultured"Microbiology Immunol. 45・2. 149-158 (2001)

赵立成：“Vi抑制野生菌株伤寒沙门氏菌培养”微生物学免疫学45·2（2001）。

Licheng,Zhao, Kawamura,Yoshiaki and Ezaki,Takayuki: "Construction of virulent defective mutants of Salmonella typhi and their phenotypic description as candidates for educational purposes"Microbio.Cult. Coll. 17. 13-21 (2001)

Li Cheng，Zhao，Kawamura，Yoshiaki 和 Ezaki，Takayuki：“伤寒沙门氏菌的强毒缺陷突变体的构建及其作为教育目的候选者的表型描述”Microbio.Cult。