Development of model system to analyze the antigen epitope to induce cell-mediated immunity in fish

开发模型系统来分析抗原表位以诱导鱼类细胞介导的免疫

基本信息

  • 批准号:
    12460088
  • 负责人:
  • 金额:
    $ 8.26万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2000
  • 资助国家:
    日本
  • 起止时间:
    2000 至 2002
  • 项目状态:
    已结题

项目摘要

Fish possess specific antigen recognition molecules as well as B and T lymphocytes and can evoke specific immune responses against a variety of antigens. In fish, however, information on cell-mediated immunity is limited when compared to those on humoral immunity due to the lack of in vivo and in vitro model systems. Vaccine against viral disease is limited in fish despite that viral diseases are serious problem in aquaculture. Development of such vaccines requires identification and characterization of viral antigens which induce protective immunity. It is well known that cell-mediated killing is most important defense mechanisms in the control of viral disease. The objective of this research project is to develop experimental model systems to analyze the protective antigens or epitopes involved in cell-mediated immunity for the development of fish vaccines.In collaboration with Dr. Okamoto at Tokyo University of Fisheries and Dr. Ototake at National Research Institute of Aquaculture … More we have established several assay systems for specific cell-mediated immunity, particularly on cytotoxic T-cell function employing clonal ginbuna and a syngeneic cell line. Those include the graft-versus host reaction (GVHR) and cell-mediated cytotoxicity (CMC) against allogeneic erythrocytes or cell lines. In the present research project we further pursued the studies and have established model systems for CMC against virus-infected cells and analyzed the protection mechanism using isogeneic ginbuna and cell lines together with fish viruses. We also extensively analyzed the MHC polymorphism in rainbow trout and found that trout cell line (RTG-2) shares the same MHC class I allele with homozygous clonal rainbow trout (C25 clone). We demonstrated MHC-restricted cell-mediated cytotoxicity against virus-infected cell lines using lymphocytes from C25 clonal trout sensitized by DNA immunization with IHNV-G or by infection with IHNV and RTG-2 cells as target. Experimental model systems established in the present study can be applied to investigate the protective antigens or epitopes involved in cell-mediated immunity for the development of fish vaccines as well as to investigate the recognition and killing g mechanism of specific cell-mediated immunity. Less
鱼类拥有特异性抗原识别分子以及 B 和 T 淋巴细胞,可以引发针对多种抗原的特异性免疫反应,然而,由于缺乏细胞介导的免疫,因此与体液免疫相比,有关细胞介导免疫的信息有限。尽管病毒性疾病是水产养殖中的严重问题,但针对鱼类的病毒性疾病疫苗的开发需要鉴定和表征诱导保护性免疫的病毒抗原。杀戮是人体最重要的防御机制该研究项目的目标是开发实验模型系统来分析细胞介导免疫中涉及的保护性抗原或表位,以开发鱼类疫苗。与东京水产大学的冈本博士和博士合作。国家水产养殖研究所的 Ototake … 更多我们已经建立了多种特定细胞介导免疫的检测系统,特别是使用克隆 ginbuna 和同源细胞系的细胞毒性 T 细胞功能,其中包括移植物抗宿主反应。在本研究项目中,我们进一步开展了研究,建立了针对病毒感染细胞的CMC模型系统,并分析了使用同基因ginbuna和细胞的保护机制。我们还广泛分析了虹鳟鱼的 MHC 多态性,发现鳟鱼细胞系 (RTG-2) 与纯合克隆具有相同的 MHC I 类等位基因。我们使用来自 C25 克隆鳟鱼的淋巴细胞(通过 IHNV-G 的 DNA 免疫或通过 IHNV 和 RTG-2 细胞作为实验靶标)证明了针对病毒感染细胞系的 MHC 限制性细胞介导的细胞毒性。本研究建立的模型系统可用于研究细胞介导免疫中涉及的保护性抗原或表位,以开发鱼类疫苗以及研究识别和杀伤机制特异性细胞介导的免疫。

项目成果

期刊论文数量(86)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Q. W. Qin, M. Ototake, H. Nagoya and T. Nakanishi: "Graft-versus-host reaction (GVHR) in clonal amago salmon, Oncorhunchus rhodurus"Vet. Immunol. Immunopathol.. 89. 83-89 (2002)
Q.W.Qin、M.Ototake、H.Nagoya 和 T.Nakanishi:“克隆 amago 鲑鱼 Oncorhunchus rhodurus 中的移植物抗宿主反应 (GVHR)”兽医。
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    0
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Nakanishi, T., U. Fischer, J.M. Dijkstra and M. Ototake: "MCH-restricted cell-mediated cytotoxicity in fish"Fisheries Science. Vol. 68, Supple. (in press).
Nakanishi, T.、U. Fischer、J.M. Dijkstra 和 M. Ototake:“鱼类中 MCH 限制的细胞介导的细胞毒性”渔业科学。
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    0
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Ototake, M., J.M. Dijkstra, I. Kiryu, Y. Yoshiura, A. Fujiwara, U. Fischer and T. Nakanishi: "Structure and functions of MHC class I genes in rainbow trout Oncorhynchus mykiss"Fish Genetics and Breeding Science. 32. 67-74 (2003)
Ototake, M.、J.M. Dijkstra、I. Kiryu、Y. Yoshiura、A. Fujiwara、U. Fischer 和 T. Nakanishi:“虹鳟 Oncorhynchus mykiss 中 MHC I 类基因的结构和功能”鱼类遗传学和育种科学。
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  • 影响因子:
    0
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  • 通讯作者:
Somamoto, T., T. Nakanishi and N. Okamoto: "Specific cytotoxic cells of ginbuna crucian carp are responsible for in vivo clearance of carp haematopoietic necrosis virus (CHNV)"Fisheries Science. Vol. 68, Supple. (in press).
Somamoto, T.、T. Nakanishi 和 N. Okamoto:“银鲫鱼的特异性细胞毒性细胞负责鲤鱼造血坏死病毒 (CHNV) 的体内清除”《渔业科学》。
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    0
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Ototake M, Okamura K,Hashimoto K,Nakanishi T.: "The function of the most primitive MHC class I"Dev.Comp.Immunol.. 24. 79 (2000)
Ototake M、Okamura K、Hashimoto K、Nakanishi T.:“最原始 MHC I 类的功能”Dev.Comp.Immunol.. 24. 79 (2000)
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NAKANISHI Teruyuki其他文献

NAKANISHI Teruyuki的其他文献

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{{ truncateString('NAKANISHI Teruyuki', 18)}}的其他基金

Development of evaluation methods for the potency of fish vaccine by simple and sensitive determination of the function of cell-mediated immunity
通过简单而灵敏地测定细胞介导的免疫功能,开发鱼疫苗效力的评价方法
  • 批准号:
    16H04984
  • 财政年份:
    2016
  • 资助金额:
    $ 8.26万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of techniques for prevention of fish disease by the analysis of IFNgamma function in fish immune response
通过分析鱼类免疫反应中的 IFNγ 功能,开发预防鱼类疾病的技术
  • 批准号:
    25292129
  • 财政年份:
    2013
  • 资助金额:
    $ 8.26万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of techniques for diagnosis and prevention of fish disease by the analysis of T cell function in fish immune response
通过分析鱼类免疫反应中的 T 细胞功能开发诊断和预防鱼类疾病的技术
  • 批准号:
    21380126
  • 财政年份:
    2009
  • 资助金额:
    $ 8.26万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
)Development of techniques for diagnosis and prevention of fish viral disease by immune gene expression profile
)开发通过免疫基因表达谱诊断和预防鱼类病毒性疾病的技术
  • 批准号:
    18380120
  • 财政年份:
    2006
  • 资助金额:
    $ 8.26万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Studies on the structure and function of tumor necrosis factor (TNF) in fish
鱼类肿瘤坏死因子(TNF)的结构与功能研究
  • 批准号:
    15380136
  • 财政年份:
    2003
  • 资助金额:
    $ 8.26万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

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