Physiological roles of cell surface ecto-enzymes

细胞表面胞外酶的生理作用

基本信息

项目摘要

Ecto-form NADase activity induced by retinoic acid (RA) in human HL-60 cells is due to CD38, which has an amino acid sequence homologous to Aplysia ADP-ribosyl cyclase. CD38 catalyzes not only the hydrolysis of NAD^+, but also the formation and hydrolysis of cyclic ADP-ribose (cADPR), that is a novel mediator or modulator of Ca^<2+> release from intracellular Ca^<2+> stores. In the present study, we investigated the functions and properties of CD38 and its related ecto-enzyme, PC-1. 1. CD38 was capable of hydrolyzing the N-glycoside bond of many compounds other than NAD^+. 2. CD38 was abundantly present in rat astrocytes in addition to lymphocytes, and the cell-surface CD38 was rapidly inactivated upon incubation with the enzyme substrates. 3. Soluble and membrane-bound forms of PC-1, which had been induced in human Jurkat T cells upon culture with a cAMP analog, were identified and characterized enzymatically and structurally. 4. Both PC-1 molecules possessed phosphodiesterase/pyrophosphatase activity, and the enzymic activity of soluble PC-1 could be utilized to search for its interacting molecules. 5. Glycosaminoglycans, such as heparin and heparan sulfate in extracellular matrix, were capable of binding to PC-1 and inhibited its phosphodiesterase activity in a manner competing with the enzyme substrates. 6. A homologue of human PC-1 was also present in C.elegans, and it possessed phosphodiesterase/pyrophosphatase activity. The enzymic activity was localized in the cell surface. Thus, PC-1 may function as an adhesion molecule to associate with glycosaminoglycans in extracellular matrix.
人视网膜酸(RA)在人HL-60细胞中诱导的ECTO形式的NADase活性是由于CD38引起的,CD38具有与Aplysia adp-ribosyl Cyclase同源的氨基酸序列。 CD38不仅催化了NAD^+的水解,还催化了环状ADP-核糖(CADPR)的形成和水解,这是Ca^<2+>释放的新型介体或调节剂,从细胞内Ca^<2+> STORES释放。在本研究中,我们研究了CD38及其相关的均酶PC-1的功能和特性。 1。CD38能够水解NAD^+以外的许多化合物的N-糖苷键。除淋巴细胞外,大鼠星形胶质细胞中大量存在CD38,并且与酶底物孵育后,细胞表面CD38迅速灭活。 3。鉴定并在酶促和结构上鉴定出了在用cAMP类似物的培养物培养的人类Jurkat T细胞中诱导的PC-1的可溶性和膜结合形式。 4。这两个PC-1分子都有磷酸二酯酶/焦磷酸酶活性,并且可利用可溶性PC-1的酶活性来搜索其相互作用的分子。 5。糖胺聚糖,例如细胞外基质中的肝素和硫酸乙酰肝素,能够与PC-1结合,并以与酶底物竞争的方式抑制其磷酸二酯酶活性。 6。元素中也存在人类PC-1的同源物,并且具有磷酸二酯酶/焦磷酸酶活性。酶活性位于细胞表面。因此,PC-1可以用作粘附分子,与细胞外基质中的糖胺聚糖相结合。

项目成果

期刊论文数量(34)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
T.Katada, et al.: "Synergistic activation of a family of phosphoinositide 3-kinase via G-protein coupled and tyrosine kinase-related receptors."Chemistry and Physics of Lipids. 98. 79-86 (1999)
T.Katada 等人:“通过 G 蛋白偶联和酪氨酸激酶相关受体协同激活磷酸肌醇 3-激酶家族。”脂质化学和物理学。
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堅田利明(分担): "シグナル伝達 総集編(竹縄忠臣 編)"羊土社. 235 (1999)
Toshiaki Katata(贡献者):“信号传输编译(竹轮忠臣编辑)”Yodosha 235(1999)。
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T.Katada et al.: "Enzymic and signal transduction properties of CD38/NADase and PC-1/phosphodiesterase"Chemical Immunology. 75 (in press). (1999)
T.Katada 等人:“CD38/NADase 和 PC-1/磷酸二酯酶的酶和信号转导特性”化学免疫学。
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KATADA Toshiaki其他文献

KATADA Toshiaki的其他文献

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{{ truncateString('KATADA Toshiaki', 18)}}的其他基金

Identification of signaling pathways involved in fungal pathogenicity and search for novel targets for antifungal drugs
鉴定真菌致病性信号通路并寻找抗真菌药物新靶点
  • 批准号:
    20K06550
  • 财政年份:
    2020
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Nutrient response mediated by a TRIM-NHL protein
TRIM-NHL 蛋白介导的营养反应
  • 批准号:
    16K14693
  • 财政年份:
    2016
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
A novel signal transduction pathway which regulates the structure of P-body and the dynamics of ARE-mRNAs
调节 P-body 结构和 ARE-mRNA 动态的新型信号转导途径
  • 批准号:
    22659015
  • 财政年份:
    2010
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Regulation of intracellular vesicle transport by small GTPase cycles
小 GTP 酶循环调节细胞内囊泡运输
  • 批准号:
    20247011
  • 财政年份:
    2008
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Membrane-Transport Signaling Involving the GTPase Cycle of G proteins
涉及 G 蛋白 GTP 酶循环的膜运输信号转导
  • 批准号:
    18207008
  • 财政年份:
    2006
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
Functional analysis of atypical G proteins involved in cell signaling network
参与细胞信号网络的非典型G蛋白的功能分析
  • 批准号:
    17079002
  • 财政年份:
    2005
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
New research initiatives in the study of G-protein signaling systems integrating cell communication network
整合细胞通讯网络的G蛋白信号系统研究新举措
  • 批准号:
    17079001
  • 财政年份:
    2005
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
The structure and function of a novel G protein family regulating eukaryotic mRNA dynamics
调节真核mRNA动态的新型G蛋白家族的结构和功能
  • 批准号:
    13854025
  • 财政年份:
    2001
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (S)
G protein-dependent vectorial transportation of receptors, ion channels, and transporters
受体、离子通道和转运蛋白的 G 蛋白依赖性载体运输
  • 批准号:
    12144202
  • 财政年份:
    2000
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research on Priority Areas
Analysis of the functions of G protein βγ-Subunit and application to drug design
G蛋白βγ亚基的功能分析及其在药物设计中的应用
  • 批准号:
    10557220
  • 财政年份:
    1998
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)

相似国自然基金

胞膜酶CD38调节巨噬细胞功能对骨稳态的影响及在骨质疏松症发病中的作用机制研究
  • 批准号:
    82372457
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    2023
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    82370210
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    2023
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    82370227
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    2023
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    48 万元
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脑血管内皮细胞CD38启动老年缺血性脑卒中血栓炎症的作用和机制研究
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    82301479
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    2023
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    30 万元
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    青年科学基金项目
抑制巨噬细胞CD38活性减轻T细胞衰老进而增强放射-免疫远隔效应的作用与机制研究
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    82373021
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    2023
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    49 万元
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    面上项目

相似海外基金

Ecto-enzyme CD38: Regulator of dendritic cell migration
胞外酶 CD38:树突状细胞迁移的调节剂
  • 批准号:
    6717757
  • 财政年份:
    2004
  • 资助金额:
    $ 4.42万
  • 项目类别:
Ecto-enzyme CD38: Regulator of dendritic cell migration
胞外酶 CD38:树突状细胞迁移的调节剂
  • 批准号:
    7039137
  • 财政年份:
    2004
  • 资助金额:
    $ 4.42万
  • 项目类别:
Ecto-enzyme CD38: Regulator of dendritic cell migration
胞外酶 CD38:树突状细胞迁移的调节剂
  • 批准号:
    6879620
  • 财政年份:
    2004
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    $ 4.42万
  • 项目类别:
Physiological functions of the cell surface antigen CD38
细胞表面抗原CD38的生理功能
  • 批准号:
    09044268
  • 财政年份:
    1997
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
Physiological functions of a novel family of nucleotide-metabolizing enzymes
新型核苷酸代谢酶家族的生理功能
  • 批准号:
    09480160
  • 财政年份:
    1997
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
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