Molecular biologic characterization of hatavirus pathogenicity

哈塔病毒致病性的分子生物学特征

• 批准号: 11694228
• 负责人: ARIKAWA Jiro
• 金额: $ 3.97万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11694228/
• 关键词: Hemorrhagic fever with renal syndrome; HFRS; hantavirus; zoonosis; virus infection; pathogenicity; genetic reassortant; infection enhancement

1. Epidemiologic conditions and status of vaccine development in China were investigated. Information regarding to nucleotide sequence of Chinese isolates and diagnostic procedures for serodiagnosis were exchanged.2. Technical information for genetic characterization of hantavirus was obtained from Slovak scientist.3. Technical information for characterization of hantavirus receptor and genetic reassortant virus was obtained from US scientist.4. Techniques for construction of artificial hantavirus was obtained from scientist of Wisconsin University School of Veterinary Medicine.5. Information regarding to Nephropathia epidemica virus pathogenicity was obtained from Dr.Antti Vaheri of Helsinki University.6. Information of epidemiologic and epizootiologic conditions in South East Asia was obtained from scientist in Thai.7. Infection enhancement by the GalNac specific lectin, DBA and SBA was confirmed. Involvement of cellular factor on the virus cell membrane fusion was elucidated.8. Pathogenicity to mice was related to growth rated at peripheral cite. The difference was considered to caused by the point mutation at enveloped protein.9. Viral S and M genome was cloned and expressed in the mammalian cells. Several kinds of mini-genomes were constructed for the examination of role for transcription and translation. For the next step, relationship between the minigenome expression and the proteins expressed by S and M genomes will be required.

1.调查我国流行病学状况和疫苗研发现状。交换了有关中国分离株的核苷酸序列和血清学诊断程序的信息。 2．汉坦病毒遗传特征的技术信息从斯洛伐克科学家处获得。3．汉坦病毒受体和基因重配病毒的表征技术信息来自美国科学家。 4．人工汉坦病毒构建技术获自威斯康星大学兽医学院科学家。 5．有关流行性肾病病毒致病性的信息来自赫尔辛基大学的Antti Vaheri 博士6。东南亚流行病学和动物流行病学状况的信息是从泰国科学家那里获得的。7。证实了 GalNac 特异性凝集素、DBA 和 SBA 增强感染的作用。阐明了细胞因子对病毒细胞膜融合的影响。8．对小鼠的致病性与外周部位的生长速率有关。认为这种差异是由包膜蛋白点突变引起的。9．病毒S和M基因组被克隆并在哺乳动物细胞中表达。构建了几种微型基因组来检查转录和翻译的作用。下一步，需要了解小基因组表达与 S 和 M 基因组表达的蛋白质之间的关系。

项目成果

Jin, H.K.et al: "Mouse Mx2 protein inhibits Hantavirus but not Influenza virus replication."Archives of Virology. 146. 41-49 (2000)

Jin, H.K. 等人：“小鼠 Mx2 蛋白抑制汉坦病毒，但不抑制流感病毒复制。”病毒学档案。

Ogino, M.et al: "N-acetylgalactosamine (GalNAc)-specific lectins mediate enhancement of Hantaan virus infection."Archives Virology. 144. 1765-1777 (1999)

Ogino, M.等人：“N-乙酰半乳糖胺 (GalNAc) 特异性凝集素介导汉坦病毒感染的增强。”档案病毒学。

Hayashi,M. et al: "Hepatic Copper Accumulation Induces DNA Strand Breaks in the Liver Cells of Long-Evans Cinnamon Strain Rats."Biochemical and Biophysical Research Communications. 276. 174-178 (2000)

林，M.

Plyusnin, A.et al: "Analysis of puumala hantavirus genome in patients with nephropathia epidemica and rodent carries from the sites of infection"Journal of Medical Virology. 59(3). 397-405 (1999)

Plyusnin, A. 等人：“流行性肾病患者和感染部位啮齿动物​​携带的普马拉汉坦病毒基因组分析”《医学病毒学杂志》。

Kariwa,H. et al: "Detection of hantaviral antibodies among patients with hepatitis of unknown etiology in Japan."Microbiology and Immunology. 44(5). 357-362 (2000)

卡里瓦，H.