Molecular epidemiology of hepatitis viruses in Indonesia

印度尼西亚肝炎病毒的分子流行病学

基本信息

批准号：
11691209
负责人：
HOTTA Hak
金额：
$ 4.74万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11691209/
关键词：
Indonesia hepatitis C virus GB virus TT virus hepatitis B virus hepatitis D virus molecularepidemiology viremia titer 遺伝子型多様性

项目摘要

Molecular epidemiological analysis was performed on hepatitis C virus (HCV), hepatitis B virus (HBV), hepatitis D virus (HDV) and possible hepatitis virus candidates, GB virus (GBV) and TT virus (TTV), in Surabaya, Indonesia.1. HCV.Mean HCV viremia titers of HCV-1b and HCV-1c were practically the same, and significantly higher than that of HCV-2a. Even among the same subtype, however, HCV viremia titers varied considerably with different HCV isolates. Sequence analysis of HCV revealed that HCV viremia titers were significantly lower when the number of amino acid mutations in the interferon sensitivity determining region (ISDR) of NS5A was four or more, compared with isolates having ISDR mutations of three or less. No other particular mutation (s) was identified in NS5A that correlated well with HCV viremia titers.2. GBV.The prevalence of serum GBV RNA was 2.7% in blood donors, 5.7% in chronic hepatitis patients, 11.5% in liver cirrhosis patients, 7.0% in hepatocellular carcinoma patien … More ts and 29.0% in hemodialysis patients in Surabaya, Indonesia. GBV posicivity was significantly higher in anti-HCV-positive blood donors than in anti-HCV-negative blood donors. No significant correlation was observed between GBV positivity and abnormal liver function tests, suggesting non-hepatopathogenic features of GBV.Phylogenetic analysis identified a novel genotype of GBV (type 5), which represented 40% of total GBV in Surabaya, Indonesia.3. TTV.The prevalence of serum TTV DNA was 33% in blood donors, 22% in chronic hepatitis patients, 24% in liver cirrhosis patients, 46% in hepatocellular carcinoma patients and 44% in hemodialysis patients in Surabaya, Indonesia. Those TTV isolates were phylogenetically classified into Gla, Glb and an as-yet-unclassifiable genotype.4. HBV.All of HBV isolates in Surabaya, Indonesia, were phylogenetically classified into genotype B.5. HDV.HDV was shown to be rather rare in Surabaya, Indonesia, with the prevalence of anti-HDV antibodies being less than 0.5%. Less

分子流行病学分析是在丙型肝炎病毒（HCV），丙型肝炎病毒（HBV），丙型肝炎病毒（HDV）以及可能的肝炎病毒候选物，GB病毒（GBV）和TT病毒（TTV），Insurabaya，Indonesia.1。 HCV-1B和HCV-1C的HCV HCV病毒血症滴度实际上是相同的，并且明显高于HCV-2A。然而，即使在同一亚型中，HCV病毒血症滴度也以不同的HCV分离株仔细而变化。 HCV的序列分析表明，当干扰素敏感性确定NS5A的氨基酸突变的数量（ISDR）的NS5A的氨基酸突变数为四个或更多时，与具有三个或更少的ISDR突变的分离株相比，HCV病毒血症滴度显着降低。在NS5A中没有发现其他特定的突变与HCV病毒血症滴度很好地相关。2。 GBV。献血者血清GBV RNA的患病率为2.7％，慢性肝炎患者的患病率为5.7％，肝硬化患者为11.5％，肝细胞癌患者的7.0％……更多的TS和29.0％的surabaya，Indonesia surabaya的血液透析患者。抗HCV阳性供血者的GBV阳性明显高于抗HCV阴性献血者。 GBV阳性和异常肝功能测试之间未观察到没有显着相关性，这表明GBV的非肝病特征。Phyolegen发育分析确定了GBV的新基因型（5型），该类型占印度尼西亚苏拉巴亚总GBV的40％。 TTV。献血者血清TTV DNA的患病率为33％，慢性肝炎患者的患病率为22％，肝硬化患者的患病率为24％，肝细胞癌患者的患者为46％，印度尼西亚苏拉巴亚的血液Lysis患者为44％。这些TTV分离株将系统发育分类为GLA，GLB和尚未尚未熟悉的基因型4。印度尼西亚苏拉巴亚的HBV分离株的HBV均被系统发育分类为基因型B.5。 HDV.HDV在印度尼西亚的苏拉巴亚证明很少见，抗HDV抗体的患病率小于0.5％。较少的