Development of Bioactive Pulp Capping Material including rhBMP-2

rhBMP-2等生物活性盖髓材料的开发

• 批准号: 11557145
• 负责人: SAITO Takashi
• 金额: $ 8.45万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11557145/
• 关键词: rhBMP-2; pulp capping material; dentin; regeneration therapy; phosphophoryn; コラーゲン; 多孔質ハイドロキシアパタイト; 象牙質形成; 歯髄; rh BMP-2; 歯科保存治療; ホスホホリン

Numerous basic experiments were conducted in order to develop a pulp capping and amputation material containing recombinant human bone morphogenetic protein (RhBMP-2) that can induce calcification, and the following results were obtained.The results of animal experiments have shown that a fibrous glass membrane can act as a BMP carrier. However, when a fibrous glass membrane was used as a carrier, chondrogenesis was active, and this was attributed to the geometric structure of the carrier. While inflammatory reactions following grafting were strong, as a BMP carrier, fibrous glass membrane was shown to be a superior carrier to bone insoluble substrate (not suitable for clinical use due to its immunogenicity). It was clear that, through improvements, a superior dentine formation factor could be developed.In vitro experiments were conducted in an attempt to determine the function of phospholine, which is thought to play an important role in dentine calcification. The results showed that the phosphate-group of bound phospholine played an important role, but when dephosphorylated, the cooperation between the carboxyl group and the phosphate group was important. It was also clarified that, unlike bound phospholine, free phospholine suppresses calcification. With regard to collagen, which is a carrier, it was shown that stabilization of collagen fibers leads to stabilized the covalent binding of phospholine and enhanced calcification induction.These findings clarified that the RhBMP-2/phospholin/collagen complex is an effective inducer of hard tissue, particularly dentine. In other words, a new pulp capping material was successfully developed.

进行了许多基本实验，以开发含有重组人骨形态发生蛋白（RHBMP-2）的纸浆封盖和截肢材料，可以诱导钙化，并获得以下结果。但是，当将纤维玻璃膜用作载体时，软骨发生活跃，这归因于载体的几何结构。虽然嫁接后的炎症反应很强，但作为BMP载体，纤维玻璃膜被证明是骨不溶性底物的出色载体（由于其免疫原性，不适合临床使用）。很明显，通过改进，可以开发出上牙本质的形成因子。在体外实验中，试图确定磷酸的功能，磷酸的功能被认为在牙本质钙化中起重要作用。结果表明，结合磷脂的磷酸组起着重要作用，但是当脱磷酸化时，羧基与磷酸基团之间的合作很重要。还澄清的是，与结合的磷脂不同，游离磷脂抑制钙化。关于胶原蛋白是一种载体，表明胶原蛋白纤维的稳定性导致稳定磷脂的共价结合和增强的钙化诱导。这些发现阐明了RHBMP-2/磷酸蛋白/胶原蛋白复合物是硬性组织的有效诱导剂，尤其是牙本质。换句话说，成功开发了一种新的纸浆上限材料。

项目成果

T.Saito et al: "In Vitro Apatite Induction by Phosphophoryn Immobilized on Modified Collagen"Journal of Bone & Mineral Research. (印刷中). (2000)

T.Saito 等人：“通过固定在改性胶原上的磷灰石进行体外诱导”《骨与矿物质研究杂志》（2000 年出版）。

TAKASHI SAITO : "In Vitro Apatite Induction by Phosphophoryn Immobilized on Modified Colagen Fibrils"Journal of Bone & Mineral Research. 15・8. 1615-1619 (2000)

TAKASHI SAITO：“通过固定在改性胶原纤维上的磷灰石诱导”《骨与矿物质研究杂志》15・8（2000）。

斎藤 隆史: "象牙質基質モデルとしてのフォスフォフォリン-再構成コラーゲン線維複合体による石灰化誘導"日本歯科保存学雑誌. 42. 776-782 (1999)

Takashi Saito：“通过磷酸蛋白重组胶原纤维复合物作为牙本质基质模型诱导矿化”，日本保守牙科杂志 42. 776-782 (1999)。

T.Saito: "In vitro apatite induction by phosphoprotein immobilized on modified collagen"J.Bone and Mineral Research. 15. 1615-1619 (2000)

T.Saito：“通过固定在改性胶原上的磷蛋白进行体外磷灰石诱导”J.Bone and Mineral Research。

T. Saito et al.: "In vitro apatite nucleation by insoluble dentin collagen"Dentin/Pulp Complex. 1. 159-161 (2002)

T. Saito 等人：“不溶性牙本质胶原蛋白的体外磷灰石成核”牙本质/牙髓复合物。