Development of New Thermoresponsive Polymer with Opposite Response and a High-LCST and Its Application to Enzymatic Active Control of Antineoplastic Enzyme

新型相反响应高LCST热响应聚合物的研制及其在抗肿瘤酶酶活性控制中的应用

• 批准号: 11555217
• 负责人: HOSHINO Kazuhiro
• 金额: $ 6.59万
• 依托单位: Toyama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11555217/
• 关键词: Immobilized enzyme; Thermoresponsive polymer; Active control; Antineoplastic enzyme; Gel; 抗消炎作; 重合; 抗消炎作用

The bioterageting technology using diffusion control method has been resent investigated. Therm-responsive gel using N-isopropyl acrylamide as a monomer play a central role part in this field. However, since the response of the swelling and constriction for the thermo-responsive gel was very late, the rapid division of medicines or antineoplastic enzymes was difficult. Moreover, the active control of enzyme was an impossible.Therefore, in this study, the novel themo-responsive polymer that can be adjusted reversibly its solubility by the change of solution temperature was developed. The hybrid enzyme was prepared by coupling the polymer around an active site of antineoplastic enzyme such as superoxide dismtase. The enzymatic reaction could be instantaneously stopped when the temperature of the reaction solution was changed from 50℃ to 70℃. Moreover, the hybrid was a reversible and the activity was adjusted by up-and-down of the reaction mixture. The enzymatic activity control system using the proposed hybrid enzyme in but also drug delivery system but also manufacturer of drug, brewing, production of intermediate product in green chemistry could be utilized in future.

Theterageting Technology Control METH方法已研究了该领域的核心角色，因为肿胀的反应和对热响应性凝胶的响应是很晚在这种螺柱中，新型的响应性聚合物通过溶液温度的变化来调整了可逆性的溶解性，使药物的快速分裂当反应溶液的温度从50°C更改为70°C时，可能会停止瞬时，此外，混合动物是不可逆的活性，是某种杂交酶，但也是药物输送系统，但也是药物的制造商，酿造，中介产物的生产将来可以利用绿色化学。

项目成果

K.Hoshino et al.: "Preparaiton of a Novel Thermo-Responsive Polymer and Its Use as a Carrier for Immobilization of Thermolvsin"J. Ferment. Bioeng.. 83. 246-252 (1999)

K.Hoshino 等人：“新型热响应聚合物的制备及其作为 Thermolvsin 固定化载体的用途”J。

Kazuhiro Hoshino: "Preparation of a New Thermo-Responsive Adsorbent with NAD+ and Its Application to Affinity Precipitation"The 5th Asia-Pacific Biochemical Engineering Conference 1999. 5. 131 (1999)

星野一宏：“新型NAD热响应吸附剂的制备及其在亲和沉淀中的应用”第五届亚太生化工程会议1999. 5. 131（1999）

Kazuhiro Hoshino: "Stimuli Responsive Polymers in Bioprocessing"Hardwood Academic Publishers GMBH, The Netherlands. 458 (2000)

Kazuhiro Hoshino：“生物加工中的刺激响应聚合物”Hardwood 学术出版社 GMBH，荷兰。

Kazuhiro Hoshino: "Preparation of Thermolysin Modified with Thermo-responsive Polymer and Its Application to Control of Enzymatic Activity"J. Biosci. Bioeng.. 90(in press). (2000)

Kazuhiro Hoshino：“热响应聚合物修饰嗜热菌蛋白酶的制备及其在酶活性控制中的应用”J。

K. Hoshino, Y. Ohsawa, T. Kurokoshi, S. Morohashi, and T. Taniguchi: "Thermocontrol of Enzyme Activity of Thermolysin Modified with Thermo-Responsive Polymers"Biotechnol. Biopro. Eng.. Vol.12(in press). (2003)

K. Hoshino、Y. Ohsawa、T. Kurokoshi、S. Morohashi 和 T. Taniguchi：“用热响应聚合物修饰的嗜热菌蛋白酶活性的热控制”生物技术。