Control of extracytoplasmic stress responses by regulated intramembrane proteolysis

通过调节膜内蛋白水解控制胞质外应激反应

• 批准号: 15370084
• 负责人: AKIYAMA Yoshinori
• 金额: $ 8.13万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15370084/
• 关键词: protease; membrane proteins; regulated intramembrane proteolysis; 表層ストレス応答; 大腸菌; タンパク質分解; ストレス応答; 膜プロテアーゼ

E.coli σ^E is activated by extracytoplasmic stress-induced two step cleavages of the anti-σ^E protein, RseA (a type II single-spanning membrane protein). We showed that RseP (formerly YaeL), an E.coli homolog of the eukaryotic S2P protease, functions as a "RIP(regulated intramembrane proteolysis)" protease that introduces the second "intramembrane" cleavage into anti-σ^E protein (RseA) following the first cleavage on the periplasmic side by DegS. Our in vivo and in vitro results demonstrated that RseP directly catalyzes proteolysis within the transmembrane sequence of RseA. We also found that RseP can cleave transmembrane sequences of some model membrane proteins that are unrelated to RseA, provided that the transmembrane region contains residues of low helical propensity. We are now addressing the molecular mechanisms of the two-step proteolysis of RseA and its regulation using in vitro reaction system reconstituted from purified components.

大肠杆菌 σ^E 是通过胞质外应激诱导的抗 σ^E 蛋白 RseA（一种 II 型单跨膜蛋白）的两步裂解来激活的。我们证明了 RseP（以前称为 YaeL），一种大肠杆菌。真核 S2P 蛋白酶的同源物，作为“RIP（调节膜内蛋白水解）”蛋白酶，引入了第二种蛋白酶我们的体内和体外结果表明，RseP 直接催化 RseA 跨膜序列内的蛋白水解。可以切割一些与 RseA 无关的模型膜蛋白的跨膜序列，前提是跨膜区域包含我们现在正在研究 RseA 两步蛋白水解的分子机制及其使用由纯化组分重建的体外反应系统的调节。

项目成果

A SecE mutation that modulates SecY-SecE translocase assembly, identified as a specific suppressor of SecY defects.

调节 SecY-SecE 易位酶组装的 SecE 突变，被确定为 SecY 缺陷的特异性抑制因子。

• 发表时间: 2003
• 期刊: J.Bacteriol. 185
• 作者: Mori;H.
• 通讯作者: H.

Kazue Kanehara: "YaeL proteolysis of RseA is controlled by the PDZ domain of YaeL and a Gln-rich region of RseA"The EMBO Journal. 22. 6389-6398 (2003)

Kazue Kanehara：“RseA 的 YaeL 蛋白水解是由 YaeL 的 PDZ 结构域和 RseA 的富含 Gln 的区域控制的”EMBO 杂志。

RseP(YaeL), an E・coli RIP protease, cleaves transmembrane sequences

RseP(YaeL)，一种大肠杆菌 RIP 蛋白酶，可切割跨膜序列

• 发表时间: 2004
• 期刊: The EMBO Journal 23
• 作者: 神谷研二;Yoshinori Akiyama
• 通讯作者: Yoshinori Akiyama