Study of roles of epithelial cell rests of Malassez in periodontal regeneration

Malassez上皮细胞残渣在牙周再生中的作用研究

• 批准号: 14370711
• 负责人: OGAWA Tetsuji
• 金额: $ 8.06万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370711/
• 关键词: epithelial cell rests of Malassez; cementum repair; periodontal tissue regeneration; 培養細胞

n vivo, to clarify the roles of epithelial cell rests of Malassez (ECRM), ECRM existed in periodontal ligament during cementum repair was investigated using morphological and immunohistochemicai approaches in experimental root resorption. At day 7, after mechanical injury, root resorption was observed and ECRM were present adjacent to the site of resorption lacunae. They were observed in periodontal ligament adjacent to site of the resorption lacunae. These ECRM were immunoreactive for bone morphogenetic protein-2. During the stage of early cementum repair, the ECRM were immunoreactive for osteopontin and ameloblastin. These findings suggested that ECRM may be related to cementum repair by activating their potential to secrete matrix proteins which have been expressed in tooth development.In vitro, we investigated the expression of alkaline phosphatase (ALPase), osteopontin (OPN), bone morphogenetic protein (BMP)-2 and BMP-4 in epithelial cells (E cells) and fibroblastic cells (F cells) derived from human periodontal ligament. Reverse transcription-polymerase chain reaction analysis showed that E cells have lower ALPase and BMP-4 mRNA levels than F cells. On the other hand, the expression of OPN mRNA in E cells was stronger than in F cells. Thus, they have different expression patterns of ALPase, BMP-4 and OPN, suggesting that ERM and mesenchymal cells in periodontal ligament may be cooperatively involved in cementum repair.

n Vivo，为了阐明Malassez（ECRM）上皮细胞静止的作用，ECRM在牙周韧带中存在于牙周韧带中，并使用形态学和免疫组织学方法在实验根部吸收中研究了牙胶修复期间的牙周韧带。在第7天，在机械损伤之后，观察了根部吸收，ECRM与吸收lacunae部位相邻。在吸收腔的部位附近的牙周韧带中观察到它们。这些ECRM对骨形态发生蛋白2具有免疫反应性。在早期牙骨质修复的阶段，ECRM对骨桥蛋白和蛋白蛋白细胞蛋白具有免疫反应性。 These findings suggested that ECRM may be related to cementum repair by activating their potential to secrete matrix proteins which have been expressed in tooth development.In vitro, we investigated the expression of alkaline phosphatase (ALPase), osteopontin (OPN), bone morphogenetic protein (BMP)-2 and BMP-4 in epithelial cells (E cells) and fibroblastic cells (F cells) derived来自人类牙周韧带。逆转录聚合酶链反应分析表明，E细胞的ALPase和BMP-4 mRNA水平低于F细胞。另一方面，E细胞中OPN mRNA的表达比在F细胞中强。因此，它们具有不同的ALPase，BMP-4和OPN的表达模式，这表明牙周韧带中的ERM和间充质细胞可能与牙骨质修复有关。

项目成果

Mouri Y, Shiba H, Mizuno N, Noguchi T, Ogawa T, Kurihara H.: "Differential gene expression of bone-related proteins in epithelial and fibroblastic cells derived from human periodontal ligament"Cell Biol Int.. 22(7). 519-524 (2003)

Mouri Y、Shiba H、Mizuno N、Noguchi T、Okawa T、Kurihara H.：“人牙周韧带来源的上皮细胞和成纤维细胞中骨相关蛋白的差异基因表达”Cell Biol Int.. 22(7)。

Mouri Y.: "Differential gene expression of bone-related proteins in epithelial and fibroblastic cells derived from human periodontal ligament"Cell Biol Int,. 22(7). 519-524 (2003)

Mouri Y.：“人牙周膜来源的上皮细胞和成纤维细胞中骨相关蛋白的差异基因表达”Cell Biol Int，。

Mouri Y, Shiba H, Mizuno N, Noguchi T, Ogawa T, Kurihara H.: "Differential gene expression of bone-related proteins in epithelial and fibroblastic cells derived from human periodontal ligament."Cell Biol Int.. 22(7). 519-524 (2003)

Mouri Y、Shiba H、Mizuno N、Noguchi T、Okawa T、Kurihara H.：“人牙周膜来源的上皮细胞和成纤维细胞中骨相关蛋白的差异基因表达。”Cell Biol Int.. 22(7)。

Hasegawa N: "lmmunohistochemical characteristics of epithelial cell rests of Malassez during cementum repair"Journal of Periodontal of Research. 38. 51-56 (2003)

长谷川 N：“牙骨质修复过程中马拉塞兹上皮细胞的免疫组织化学特征”牙周研究杂志。

Hasegawa N, Kawaguchi H, Ogawa T, Uchida T, Kurihara H.: "Immunohistochemical characteristics of epithelial cell rests of Malassez during cementum repair"Journal of Periodontal Research. 38. 51-56 (2003)

长谷川 N、川口 H、小川 T、内田 T、栗原 H.：“牙骨质修复过程中马拉塞斯上皮细胞残骸的免疫组织化学特征”牙周研究杂志。