Development of a noble method for utilization of organic waste resources, open lactic acid fermentation of kitchen refuse is a good model of it

开发有机废弃物资源化利用的高尚方法，餐厨垃圾开放式乳酸发酵就是一个很好的典范

基本信息

批准号：
14360202
负责人：
SAKAI Kenji
金额：
$ 7.94万
依托单位：
Oita University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

In order to show new direction for investigation of economical transformation of organic waste, we set three subjects in the none-sterilizing lactic acid(LA) fermentation of garbage ; analyses of microbes by FISH, mixed culture reactor system with plural substrates, and high-viscous, suspended fermentation system.(1)Quantitative fluorescent In Situ hybridization(FISH) using group- and species- specific probes has established, and selective LA accumulation in the pH-swing control fermentation was found to be caused by L.plantarun, which also resulted decrease of optical purity of LA. On the other hand, inoculation of thermophilic L-LA producing bacteria at 55℃ was effective to accumulation of L-LA. FISH analysis demonstrated well the relationship between LA purity and populations of mesophilic and thermophilic LA bacteria.(2)To determine conditions for supporting growth of a specific microorganism, a new co-culture system with three microfiltration modules, fermentors, and a mixing vess … More el was developed. The co-culture system allowed the cultivation of the individual microorganism in each fermentor with the successful exchange of medium. Efficient production of LA from mixture of xylose and glucose by co-culture with two microorganisms also succeeded. In a simultaneous saccharification and fermentation of unsterile defatted rice bran, a LA bacterium could grow specifically by keeping the pH acidic, with selective LA production.(3)In LA fermentation by Rhizopus oryzae, the high viscosity-suspension system was examined by reducing the water content, however, oxygen supply required for the fermentation was not enough and the LA yield lowered. Product inhibition is strong in the continuous fermentation of glucose. Although starch did not inhibit the fermentation, there was a limit in concentration due to its viscosity. As a result, addition of equivalent water was desirable in kitchen refuse fermentation. Also, it was considered that the substrate containing starch highly should be supplied in batch to the system. Less

为了给有机垃圾的经济化转化研究指明新的方向，我们在垃圾非灭菌乳酸（LA）发酵中设置了三个课题：FISH微生物分析、多元基质混合培养反应器系统和高通量微生物分析。 (1)建立了使用群体和物种特异性探针的定量荧光原位杂交(FISH)，并发现pH波动控制发酵中选择性LA积累是由以下因素引起的： L.plantarun，也导致LA的光学纯度降低。另一方面，在55℃下接种嗜热L-LA生产菌对L-LA的积累有效，FISH分析很好地证明了LA纯度之间的关系。 (2)为了确定支持特定微生物生长的条件，开发了一种具有三个微过滤模块、发酵罐和混合容器的新共培养系统。共培养系统允许在每个发酵罐中培养单个微生物，并成功更换培养基，通过与两种微生物共培养，从木糖和葡萄糖的混合物中高效生产 LA。在脱脂米糠中，一种LA细菌可以通过保持pH呈酸性而特异生长，选择性地生产LA。(3)米根霉发酵LA时，高产率通过降低水含量来考察粘度悬浮系统，但发酵所需的氧气供应不足，并且在葡萄糖的连续发酵中，产物抑制作用较强，尽管淀粉不抑制发酵，但存在。由于其粘度，其浓度受到限制，因此，在厨房垃圾发酵中需要添加等量的水，并且认为应该向系统中批量供应高含量的水。