Studies on Cyanobacterial Cyderophores

蓝藻细胞细胞的研究

基本信息

批准号：
14360120
负责人：
MURAKAMI Masahiro
金额：
$ 9.47万
依托单位：
KYORITSU WOMEN'S UNIVERSITY (2003)The University of Tokyo (2002)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

Cyanobacteria are the prokaryotic phytoplankton and the representative primary producer in aquatic environment. They perform oxygenic photosynthesis using various iron-containing catalysis, and many species of them can also fix nitrogen by the iron-rich enzyme, nitrogenase. However little is known about the molecular mechanism to acquire the iron of cyanobacteria. In this report, I reported the first genuine Cyanobacterial siderophores from the freshwater cyanobacteria with nitrogen fixation ability.1. Screening for siderophore productive activity of various freshwater Cyanobacterial speciesFor large-scale screening, 41 axenic strains of freshwater cyanobacteria were selected and obtained from four culture collections, NIES, PCC, UTEX and IAM. In this screening, all strains of Microcystis aeruginosa did not show siderophore productive activity. The other noticeable result in this screening was that most of heterocystous diazotrophic species produced siderophores. This result gave me a … More hypothesis that heterocystous species had the faculty of producing siderophores to maintain their nitrogenase, an iron-rich protein, for nitrogen fixation.2. Anachelins, novel siderophores produced by Anabaena cylindrica NIES-19Novel Cyanobacterial siderophores, Anachelin-2 and Anachelin were isolated from Anabaena cylindrica NIES-19. The molecular formula of these compounds were determined to be C35H49013N6 from HRFAB-MS analysis. These structures were decided 2D NMR analyses including COSY, HMQC, 1H-13C HMBC, and 1H-15N HMBC experiments.3. Schizokinen, a siderophore produced by two strains of Anabaena variabilis M-204 and NIES-23Isolated siderophores from A. variabilis(M-204, NIES-23) were also positive in the Csaky test, indicating that this compound was a hydroxamate-type siderophore, schizokinen. The structure of schizokinen was confirmed by NMR analysis.4. Oscillabactin, a novel siderophore produced by Oscillatoria tenuis UTEX1566We isolated a novel Cyanobacterial siderophore, oscillabactin from Oscillatoria tenuis UTEX 1566. Oscillabactin is the first siderophore containing the hydroxypyridinone structure as the iron-binding group. Less

蓝细菌是原核浮游植物，是水生环境中代表性的初级生产者，它们利用各种含铁催化进行产氧光合作用，其中许多物种还可以通过富铁酶固氮酶来固氮，但人们对这种分子知之甚少。在这篇报告中，我报道了第一个来自具有固氮作用的淡水蓝藻的真正的蓝藻铁载体。 1.各种淡水蓝藻菌种铁载体生产活性的筛选为了进行大规模筛选，从NIES、PCC、UTEX和IAM四个培养物保藏中心中筛选出41株淡水蓝藻无菌菌株。铜绿假单胞菌没有表现出铁载体生产活性，该筛选中的另一个值得注意的结果是大多数异囊固氮物种产生。这个结果给了我一个假设，即异囊物种具有产生铁载体的能力，以维持其固氮酶（一种富含铁的蛋白质），从而实现固氮。2。从Anabaena cylindrica NIES-19中分离得到Anachelin-2和Anachelin，并确定了这些化合物的分子式。经HRFAB-MS分析，这些结构被确定为C35H49013N6，包括COSY、HMQC、1H-13C HMBC和1H-15N HMBC实验。3． NIES-23 从 A 中分离出铁载体。 variabilis(M-204, NIES-23)在Csaky试验中也呈阳性，表明该化合物是异羟肟酸型铁载体，裂殖菌素。经NMR分析证实了裂殖菌素的结构。4． Oscillabactin UTEX1566我们从蓝藻中分离出一种新型蓝藻铁载体，oscillabactin Oscillabactin 是纤细颤藻 UTEX 1566。 Oscillabactin 是第一个含有羟基吡啶酮结构作为铁结合基团的铁载体。