Functions and Mechanisms of Action of the Medaka Sex-Determining Gene

青鳉性别决定基因的功能和作用机制

基本信息

批准号：
14340259
负责人：
NAGAHAMA Yoshitaka
金额：
$ 9.02万
依托单位：
Okazaki National Research Institutes, National Institute for Basic Biology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14340259/
关键词：
Fish Sex-determining gene Testis Sertoli cell Sex differentiation Medaka DMY DMRT1 性決定精巣分化 Y染色体脊椎動物特異抗体

项目摘要

We previously identified PG17 as the leading candidate for the medaka sex-determining gene. To definitively identify PG17 as a sex-determining gene, we injected one-cell stage medaka embryos with a genomic DNA fragment carrying PG17. The transgenic embryos were bred until secondary sexual characters were observed. We found that all XX transgenic fish were male, showing that PG17 is sufficient to induce male development. The present data combined with our previous data indicate that PG17 is the sex-determining gene of medaka and named it DMY.DMY mRNA and protein are expressed specifically in the somatic cells (Sertoli cells) surrounding primordial germ cells (PGC) in the early gonadal primordium, before morphological sex differences are apparent. DMRT1, another DM domain gene that is also involved in male development in other vertebrates, is expressed in spermatogonium-supporting cells after testicular differentiation (20-30 days after hatching). These results strongly suggest that DMY regulates PGC proliferation and differentiation sex-specifically during early gonadal differentiation of XY individuals and that DMRT1 regulates spermatogonial differentiation.Because Sry transgenic mice are sterile and because DMY transgenic XX medakas are fertile, i.e., fully functional males, DMY transgenic medakas are the first transgenic vertebrates known to undergo complete sex reversal.

我们以前以前将PG17确定为Medaka性别确定基因的领先候选者。为了确定地将PG17识别为性别确定的基因，我们用带有PG17的基因组DNA片段注射了一细胞阶段的Medaka胚胎。育成转基因胚胎，直到观察到继发性特征为止。我们发现所有XX转基因鱼都是雄性，表明PG17足以诱导男性发育。目前的数据与我们先前的数据相结合，表明PG17是Medaka的性别确定基因，并将其命名为DMY。dmy mRNA和蛋白质在原始细胞（Sertoli细胞）中特别表达了原始生殖细胞（PGC）早期的性腺原发性生殖细胞（PGC），然后在形态性别差异之前，在形态性别差异之前。 DMRT1是另一种DM结构域基因，也参与其他脊椎动物中的男性发育，在睾丸分化后（孵化后20-30天）在精子支持细胞中表达。这些结果强烈表明，DMY在XY个体的性腺早期分化过程中调节PGC的增殖和分化性别，并且DMRT1调节精子差异化，因为Sry转基因小鼠是无菌的，因为DMY转基因MEDAKAS是肥沃的。逆转。