Dynamism of plastid gene expression by intracellular communication

细胞内通讯的质体基因表达动态

• 批准号: 14340252
• 负责人: SUGITA Mamoru
• 金额: $ 10.5万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14340252/
• 关键词: Chloroplast; Physcommitrella patens; Plastid transformation; Relic gene; arginine tRNA gene; Plastid RNA polymerase; plastid sigma factor; PPR protein; RNA編集; タバコ; DNAチップ; 概日リズム; Lhcb遺伝子; ミトコンドリア; 翻訳開始コドン; シグマ因子; ファージ型RNAポリメラーゼ

This research purpose is to define the network of gene expression by communication between organelles and nuclear genomes within a plant cell. The novel findings obtained are as follows.1.We constructed a plastid DNA chip for microarray analysis of plastid genes from the moss, Physcomitrella patens.2.We reported phylogenetic analyses using 51 genes from the entire plastid genome sequences of 20 representative plant species. This indicated that extant bryophytes (mosses, liverworts, and hornworts) form a monophyletic group with high statistical confidence and that extant bryophytes are likely sisters to extant vascular plants. We propose bryophyte monophyly as the current best hypothesis.3.We developed plastid transformation technique for the moss, Physcomitrella patens and constructed stable plastid trnR-CCG knockout moss transformants. The trnR-CCG knockout transformants indicated that the P.patens trnR-CCG gene is not essential for plastid function.4.We identified a nuclear gene, PpR … More poA, encoding the alpha subunit of plastid-encoded plastid RNA polymerase (PEP). We generated and characterized PpRpoA knockout mosses. Primer extension, northern blot, run-on transcription assay and in vitro transcription assays first demonstrated that both PEP and NEP(nuclear-encoded plastid RNA polymerase) exist in the moss and recognize the common promoters of photosynthesis genes and non-photosynthesis genes.5.We identified three PpSig genes encoding plastid sigma factor. Among the three PpSig genes, only PpSig5 was clearly controlled by the circadian clock and/or by blue light signaling in the moss P.patens.6.An extensive survey of the Physcomitrella expressed sequence tag(EST) databases revealed 36 ESTs encoding pentatricopeptide repeat(PPR) motif-containing proteins. We further characterized five full-length cDNAs encoding plastid-localized PPR proteins, PPR513-10 and PPR566-6 were expressed differentially in protonemata grown under different light-dark conditions, suggesting they have distinctive functions in chloroplasts. This is the first report and analysis of genes encoding PPR proteins in bryophytes. Less

该研究目的是通过细胞细胞内的细胞器和核基因组之间的通信来定义基因表达网络。获得的新发现如下。1。我们构建了一种质体DNA芯片，用于从苔藓，Physcomitrella patens的质体基因的微阵列分析。2。我们报道了使用来自20种代表性植物物种的整个质体基因组序列的51个基因进行系统发育分析。这表明，广泛的苔藓植物（苔藓，肝脏和霍恩沃尔特）形成一个具有高统计信心的单系组，并且扩展的苔藓植物很可能是延伸的血管植物的姐妹。我们提出了单脂肪作为当前的最佳假设。3。我们为苔藓，Physcomitrella patens开发了质体转化技术，并构建了稳定的质体TRNR-CCG敲除苔藓转化体。 TRNR-CCG敲除转化体表明，P.patens TRNR-CCG基因对于塑料功能并不是必不可少的。4。我们确定了一个核基因，PPR…更多POA，编码Plastid plastid的Plastid Plastid Plastid RNA Polymerase（PEP）的Alpha亚基。 Primer extension, north blot, run-on transcription assay and in vitro transcription assays first demonstrated that both PEP and NEP(nuclear-encoded plastid RNA polymerase) exist in the moss and recognized the common promoters of photosynthesis genes and non-photosynthesis genes.5.We identified three PpSig genes encoding plastid sigma factor.在三个ppsig基因中，只有ppsig5由昼夜节律和/或苔藓p.patens中的蓝光信号清楚地控制。6。对Physcomitrella表达的序列TAG（EST）数据库进行的广泛调查揭示了编码五肽重复（PPR）含有含有蛋白质的pentatricopeptide retectects蛋白。我们进一步表征了五个编码质体定位的PPR蛋白PPR513-10和PPR566-6的全长cDNA在不同的光黑暗条件下生长的质子膜中的表达不同，这表明它们在叶绿体中具有独特的功能。这是对苔藓植物中编码PPR蛋白的基因的第一个报告和分析。较少的

项目成果

Nakamura, T., Furuhashi, Y., Hasegawa, K., Hashimoto, H., Obokata, J., Sugita, M., Sugiua, M.: "Array-based analysis on tobacco plastid transcripts : preparation of a genomic microarray containing all genes and intergenic regions"Plant Cell Physiol. 44. 8

Nakamura, T.、Furuhashi, Y.、Hasekawa, K.、Hashimoto, H.、Obokata, J.、Sugita, M.、Sugiua, M.：“基于阵列的烟草质体转录物分析：基因组微阵列的制备

Chloroplast phylogeny indicates that bryophytes are monophyletic.

• DOI: 10.1093/molbev/msh203
• 发表时间: 2004-10
• 期刊: Molecular biology and evolution
• 影响因子: 10.7
• 作者: T. Nishiyama;P. Wolf;M. Kugita;R. Sinclair;M. Sugita;C. Sugiura;T. Wakasugi;Kyoji Yamada;K. Yoshinaga;K. Yamaguchi;K. Ueda;M. Hasebe

Aoki, S., Kato, S., Ichikawa, K., Shimizu, M.: "Circadian expression of the PpLhcb2 gene encoding a major light-harvesting chlorophyll a/b-binding protein in the moss Physcomitrella patens"Plant Cell Physiol.. 45. 68-76 (2003)

Aoki, S.、Kato, S.、Ichikawa, K.、Shimizu, M.：“编码小立碗藓中主要捕光叶绿素 a/b 结合蛋白的 PpLhcb2 基因的昼夜节律表达”植物细胞生理学。

Miyata, Y., Sugiura, C., Kobayashi, Y., Hagiwara, M., Sugita, M.: "Chloroplast ribosomal S14 protein transcript is edited to create a translation initiation codon in the moss Physcomitrella patens"Biochim. Biophys. Acta. 1576. 346-349 (2002)

Miyata, Y.、Sugiura, C.、Kobayashi, Y.、Hagiwara, M.、Sugita, M.：“编辑叶绿体核糖体 S14 蛋白转录物，以在苔藓立碗藓中创建翻译起始密码子”Biochim。

Differential expression on a daily basis of plastid sigma factor (PpSig) genes from the moss Physcomitrella patens : Regulatory interactions among PpSigs, the circadian clock and blue light signaling mediated by cryptochromes.

来自苔藓小立碗藓的质体西格玛因子 (PpSig) 基因的日常差异表达：PpSigs、生物钟和由隐花色素介导的蓝光信号之间的调节相互作用。

• 发表时间: 2004
• 期刊: Plant Physiol. 136
• 作者: Ichikawa;K.;Sugita;M.;Imaizumi;T.;Wada;M.;Aoki;S.
• 通讯作者: S.