Central distribution of neuronal cell bodies innervating the levator veli palatini muscle and its myosin heavy chain isoform expression pattern - for development of a new palate repair procedure -

支配提腭肌的神经元细胞体的中央分布及其肌球蛋白重链亚型表达模式 - 用于开发新的上颚修复程序 -

基本信息

批准号：
11470444
负责人：
YASUDA Kouichi
金额：
$ 9.02万
依托单位：
MATSUMOTO DENTAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

The levator veli palatini (LVP) muscle has a very important role in the complex functions regulating velopharyngeal mechanisms. Previous studies indicate that the LVP muscle is innervated by the glossopharyngeal nerve which is contained efferent and afferent component, and LVP motoneurons are localized in the ambiguous nucleus, and afferen neurons are projected to nucleus solitary tract. In this study, we shall present ; First, in more tooled on the central distribution of the LVP motoneurons and afferent terminals, and investigated location of LVP afferent neuronal cell bodies by using electrophysiological and horseradish peroxidase (HRP)- tracing techniques ; Second, serial muscle cross-sections using monoclonal antibodies specific for myosin heavy chain to characterize the muscle fibers of the LVP muscle HRP-labeled motoneurons in the ambiguous nucleus were separated two rostrocaudally components which were distinguish between rostral division and caudal division, in the rostral division correspond the Botzinger Complex and caudal division correspond rostral ventral respiration group. HRP-labeled afferent neuronal cell bodies were observed in the glossopharyngo-vagal complex ganglion, and HRP-labeled afferent terminals were observed in the bilateral lateral region of nucleus solitary tract. THe LVP muscle was shown to be composed of two muscle compartments by myosin heavy chain isoform expression pattern ; one showed no staining for slow myosin heavy chain isoform but reacted positively for the fast myosin heavy chain isoform, while the other was stained positively for both fast and slow myosin heavy chain isoform. These results suggest it was minutely invested relationship between the myosin heavy chain isoform expression pattern and central distribution of LVP motoneurons or central projections of afferent neurons with regard to active both inspiration and expiration of the LVP muscle.

提腭腭肌 (LVP) 在调节腭咽机制的复杂功能中具有非常重要的作用。既往研究表明，LVP肌受舌咽神经支配，舌咽神经包含传出和传入成分，LVP运动神经元位于模糊核，传入神经元投射到核孤束。在本研究中，我们将介绍；首先，更多地研究 LVP 运动神经元和传入神经末梢的中心分布，并通过使用电生理学和辣根过氧化物酶 (HRP) 示踪技术研究 LVP 传入神经元细胞体的位置；其次，使用肌球蛋白重链特异性单克隆抗体进行连续肌肉横截面，以表征模糊核中 LVP 肌肉 HRP 标记的运动神经元的肌纤维，将两个头尾部分分开，在头端部分区分头侧分裂和尾侧分裂。分裂对应于 Botzinger 复合体，尾部分裂对应于吻端腹侧呼吸组。在舌咽-迷走神经复合神经节中观察到HRP标记的传入神经元细胞体，在核孤束的双侧外侧区域中观察到HRP标记的传入神经末梢。肌球蛋白重链亚型表达模式显示 LVP 肌肉由两个肌肉区室组成；一种对慢肌球蛋白重链同种型没有染色，但对快肌球蛋白重链同种型呈阳性反应，而另一种对快肌球蛋白重链同种型和慢肌球蛋白重链同种型均呈阳性染色。这些结果表明，肌球蛋白重链同工型表达模式与 LVP 运动神经元的中心分布或传入神经元的中心投射之间存在密切关系，与 LVP 肌肉的主动吸气和呼气有关。