Reproduction structure of an old-growth evergreen broad-leaved forest analyzed by microsatellite marke

微卫星标记分析古老常绿阔叶林的繁殖结构

• 批准号: 11460069
• 负责人: YAMAMOTO Shin-ichi
• 金额: $ 7.81万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11460069/
• 关键词: Evergreen broad-leaved forest; Old-growth forest; <Castanopsis>___________- <cuspidata>_________-; Heterozygosiry; Microsatellite; Genetic marker; DNA; Forest dynamics; ツバキ; Evergreen broad-leaved forest; Old-growth forest; <Castanopsis>___________- <cuspidata>_________-; Heterozygosiry; Microsatellite; Genetic marker; DNA; Forest dynamics; ツバキ

Structure and dynamics of old-growth, evergreen broad-leaved forest community and tree populations were studied in the Tatera Forest Reserve using demographic and molecular biological methods. The population structure was different between co-dominant species, Castanopsis cuspidate var. sieboldii and Distylium racemosum. The spatial distribution pattern of major tree species seemed to be determined rather biological factors such as inter/intra-species competition than environmental factors such as canopy gap and topography. Canopy and tree community dynamics parameters were in balance, indicating this forest being in dynamic-equilibrium. Mortality and recruitment of trees depend on canopy gap characteristics, resulting in that canopy dynamics originated from natural disturbances influence tree composition and community dynamics. The microsatellite markers, which can detect the genetic structure, gene flow and be useful tools for paternity analysis, were developed in Camellia japonica a … More nd C. cuspidata var. sieboldii. For C. japonica, four microsatellite markers were developed. C. japonica were clumped in the stand and weak genetic structure were detected. There was strong genetic structure over short-distance between individuals. Limited seed dispersal and heterogeneity of microsite of stand may influence spatial pattern and genetic structure of C. japonica. For C. cuspidata var. sieboldii, six newly developed microsatellite markers and two markers which were already developed in Quercus species showed high polymorphism and can be useful for reproduction ecology. Sprout regeneration from damaged canopy stem seemed to be important for C. cuspidata var. sieboldii in spite of large amount of saplings. Thus, the spatial structure and dynamics of forest tree community were detected using demographic methods and extend the knowledge of the genetic structure and reproduction ecology for major tree species in evergreen broad-leaved forest using molecular biological methods. I Less

使用人口统计学和分子生物学方法研究了旧生长，常绿阔叶林体和树木种群的结构和动态。共同主导物种，Castanopsis cuscuttiate var之间的种群结构不同。 sieboldii和distylium racemosum。与环境因素（例如冠层间隙和地形）相比，主要树种的空间分布模式似乎是生物学因素（例如间/种内竞争）。顶篷和树木群落动态参数处于平衡状态，表明该森林处于动态平衡状态。树木的死亡率和募集取决于冠层间隙特征，从而导致冠层动态源自自然灾害，影响树木组成和社区动态。可以检测遗传结构，基因流量和用于patanity分析的有用工具的微卫星标记是在山茶中开发的。 Sieboldii。对于Japonica，开发了四个微卫星标记。 japonica聚集在林分中，并检测到弱遗传结构。个人之间的短途遗传结构很强。林分微材料的种子分散和异质性可能会影响Japonica的空间模式和遗传结构。用于C. cuspidata var。 Sieboldii，六个新开发的微卫星标记和两个已经在槲皮物种中开发的标记，这些标记显示出高多态性，可用于生殖生态学。受损的树冠茎的发芽再生似乎对C. cuspidata var很重要。 Sieboldii尽管有大量的树苗。这是使用人口统计学方法检测到的森林树群落的空间结构和动态，并扩展了使用分子生物学方法中常绿阔叶森林中主要树种的遗传结构和繁殖生态学的知识。我少