Studies on pollen coat proteins involved in pollen-stigma interactions in Brassica sp.

甘蓝属植物花粉外壳蛋白参与花粉柱头相互作用的研究。

基本信息

批准号：
11460056
负责人：
TAKAYAMA Seiji
金额：
$ 9.54万
依托单位：
Nara Institute of Science and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11460056/
关键词：
self-incompatibility pollen Brassica PCP SP11 SLR1-BP primary incompatibility adhesion 花粉表層蛋白質 SLP1-BP 種間認識反応自家不和合成 SLG SRK 花粉側因子

项目摘要

Plant sexual reproduction depends on highly specific interactions between pollen and pistil. Pistils can recognize to select from the genetically diverse range of pollen arriving on the stigma so that only appropriate pollen effects fertilization. These systems include barriers to cross-species fertilization and intraspecific barriers such as self-incompatibility (SI) that prevent self-fertilization.In Brassica, stigmaticaly expressed members of a protein family, SLG family, are expected to play central roles in these recognition systems. SRK (S-receptor kinase) and SLG (S-locus glycoprotein), are shown to be involved in the recognition of self/non-self pollen in SI.SLR1 is suggested to be involved in the initial stages of pollen-stigma adhesion. Although these familial proteins are expected to interact with some components on pollen grains, the molecular nature of these components remained elusive.In this study, we searched for these interacting molecules in PCP (pollen coat protein) family of proteins and succeeded to identify two key component named SP11 and SLR1-BP.SP11, which is encoded in the self-incompatibility (S) locus and belongs to a novel class of PCP family, is tumed out to be the pollen determinant of SI.We presented experimental evidence showing that SP11 is working as a self/non-self recognition marker which is perceived by SRK on stigmatic papillar cells. SLR1-BP, which is a new member of PCP class A proteins, is interacting with SLR1 with high affinity. Although the biological signficance of SLR1-BP is as yet to be exprimentally demonstrated, the interaction between SLR1 and SLR1 -BP is expected to be involved in the pollen-stigma adhesion process. These two pollen components identified in this study should be key molecules in understanding the pollen-stigma recognition process.

植物有性繁殖取决于花粉和雌蕊之间的高度特异性相互作用。雌蕊可以识别出从污名上的花粉范围从遗传多样的范围中进行选择，从而只有适当的花粉对受精的影响。这些系统包括跨物种受精的障碍和种内障碍，例如自我兼容（SI），以防止自我受精。 SRK（S受体激酶）和SLG（S-核糖蛋白）被证明参与了SI.SLR1中自我/非自然花粉的识别，建议与花粉粘液粘附的初始阶段有关。 Although these familial proteins are expected to interact with some components on pollen grains, the molecular nature of these components remained elusive.In this study, we searched for these interacting molecules in PCP (pollen coat protein) family of proteins and succeeded to identify two key component named SP11 and SLR1-BP.SP11, which is encoded in the self-incompatibility (S) locus and belongs to a novel class of PCP family,我们提出了SI的花粉决定因素。我们提供了实验证据，表明SP11是一种自我/非自我识别标记，SRK在污名化的乳头细胞上感知到了自我/非自身识别标记。 SLR1-BP是PCP A类蛋白的新成员，正在与SLR1相互作用，具有高亲和力。尽管SLR1-BP的生物学标志性尚未得到证明，但SLR1和SLR1-BP之间的相互作用有望参与花粉粘附过程。这项研究中确定的这两个花粉成分应该是理解花粉粘液识别过程的关键分子。