Development of a peptide-vaccine for prevention of the surface caries.

开发用于预防表面龋齿的肽疫苗。

• 批准号: 10470397
• 负责人: NISIZAWA Tosiki
• 金额: $ 3.84万
• 依托单位: National Institute of Infections Diseases
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10470397/
• 关键词: peptide vaccine; dental caries; cell surface protein antigen; Streptococcus mutans; synthetic peptide; 鼻腔免疫; 粘膜免疫; 滑面う蝕; 多面ペプチドワクチン; アグレトープ; スペーサーアミノ酸; コンジェニックマウス; 抗体誘導能; 交叉反応性エピトープ

In general, the most advantage of a peptide vaccine is its prominent safety. Peptide can be automatically and freely synthesized in accordance with the design for a desired antigenic epitope. Thus, unnecessary and inconvenient epitopes can be easily avoided from the vaccine.In the course of developing a synthetic peptide vaccine for dental caries, we identified a unique13-mer peptide, TYEAALKQYEADL, as a minimum antigenic unit peptide inducing cross-inhibiting antibodies to a cell surface protein antigen of Streptococcus mutans. However, the immunogenicity of a small peptide is usually very weak and strictly controlled by MHC class II restriction. To develop a peptide vaccine, it is very important to improve the immunogenicity of peptides and to escape from the MHC restriction. In this research project, we have succeded in developing the original design(OMP-KK-U) covering the disadvantages of peptide antigens for a human being on the basis of the data from animal experiments using B10 congenic mice.

通常，肽疫苗的最优势是其明显的安全性。肽可以根据所需抗原表位的设计自动自由合成。因此，可以从疫苗中很容易避免不必要和不便的表位。在开发牙科龋齿的合成肽疫苗的过程中，我们确定了一种独特的13-mer肽Tyeakalkqyeadl，作为诱导最小抗原的抗原肽对抗原抗体的抗生素肽对抗原抗原的抗原肽诱导的抗原抗体抑制剂，链球菌突变的细胞表面蛋白抗原。但是，小肽的免疫原性通常非常弱，并且严格受到MHC II类限制的控制。为了开发肽疫苗，提高肽的免疫原性并摆脱MHC限制非常重要。在该研究项目中，我们成功地开发了原始设计（OMP-KK-U），该设计涵盖了使用B10先天小鼠的动物实验的数据，涵盖了人类肽抗原的缺点。

项目成果

Takeuchi H., H.Senpuku, K.Matin, N.Kaneko, N.Yusa, E.Yoshikawa, H.Ida, S.Imai, T.Nisizawa, Y.Abei, Y.Kono, T.Ikemi, Y.Toyoshima, K.Fukushima, N.Hanada: "New dental drug delivery system for removing mutans streptococci from the oralcavity : effect on oral

Takeuchi H.、H.Senpuku、K.Matin、N.Kaneko、N.Yusa、E.Yoshikawa、H.Ida、S.Imai、T.Nisizawa、Y.Abei、Y.Kono、T.Ikemi、Y.

H.Kato,H.Takeuchi, Y.Oishi, H.Senpuku, N.Shimura, N.Hanada and T.Nisizawa: "The immunogenicity of various peptide antigens inducing cross-reacting antibodies to a cell surfface protein antigen of Strepotococcus mutans." Oral microbiology and Immunology. (

H.Kato、H.Takeuchi、Y.Oishi、H.Senpuku、N.Shimura、N.Hanada 和 T.Nisizawa：“各种肽抗原的免疫原性诱导抗体与变形链球菌细胞表面蛋白抗原发生交叉反应。

Kato,H.,H.Takeuchi,Y.Oishi,H.Senpuku,N.Shimura,N.Hanada and T.Nisizawa,: "The immunogenicity of various peptide antigens inducing cross-reacting antibodies to a cell surface protein antigen of Streptococcus mutans."Oral Microbiol.Immunol.. 14. 213-219 (19

Kato，H.，H.Takeuchi，Y.Oishi，H.Senpuku，N.Shimura，N.Hanada 和 T.Nisizawa，：“诱导与链球菌细胞表面蛋白抗原交叉反应的抗体的各种肽抗原的免疫原性

Oishi,Y.,A.Onozuka,H.Kato,N.Shimura,S.Imai,T.Nisizawa.: "The effect of amino acid spacers on the antigenicity of dimeric peptide-inducing cross-reacting antibodies to a cell surface protein antigen of Streptococcus mutans"Oral Microbiol Immunol.. 16. 40-4

Oishi,Y.,A.Onozuka,H.Kato,N.Shimura,S.Imai,T.Nisizawa.：“氨基酸间隔物对二聚肽诱导交叉反应抗体对细胞表面蛋白的抗原性的影响

Takeuchi H., H. Senpuku, K. Matin, N. Kaneko, N. Yusa, E. Yoshikawa, H. Ida, S. Imai, T. Nisizawa, Y. Abei, Y. Kono, T. Ikemi, Y. Toyoshima, K. Fukushima, N. Hanada: "New dental drug delivery system for removing mutans streptococci from the oralcavity : e

Takeuchi H.、H. Senpuku、K. Matin、N. Kaneko、N. Yusa、E. Yoshikawa、H. Ida、S. Imai、T. Nisizawa、Y. Abei、Y. Kono、T. Ikemi、Y.