Study on B cell differentiation signaling by using a novel system

利用新型系统研究 B 细胞分化信号传导

• 批准号: 10470091
• 负责人: KARASUYAMA Hajime
• 金额: $ 8.45万
• 依托单位: Tokyo Medical and Dental University (2000)Tokyo Metropolitan Organization for Medical Research (1998-1999)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10470091/
• 关键词: per B cell receptor (preBCR); B cell differentiation; pro B cell; pre B cell; Igβ; signal transduction; gene cloning; raft; 免疫グロブリン遺伝子再構成; 胚型転写物; 免疫グロプリン遺伝子再構成; 急性リンパ性白血病

Pre B cell receptor (preBCR) is expressed transiently at the early stage of B cell development and plays an important role in B cell differentiation. It remains to be determined what kind of signals are delivered from preBCR to induce B cell differentiation. In this study, we examined the preBCR signal transduction pathway by using a novel system which we have originally developed. We purified and sequenced a 36 kD protein (pp36) that was tyrosine-phosphorylated by preBCR signaling. The cloning of a corresponding cDNA revealed that pp36 is a novel protein with lipid modification at the N-terminal region. Since this lipid modification is know to be essential for proteins to anchor into lipid raft, pp36 appears to play an important role in signal transduction.

前B细胞受体（preBCR）在B细胞发育早期短暂表达，在B细胞分化中发挥重要作用。 preBCR 传递何种信号来诱导 B 细胞分化仍有待确定。在这项研究中，我们使用我们最初开发的新系统检查了 preBCR 信号转导途径。我们纯化并测序了 36 kD 蛋白质 (pp36)，该蛋白质通过 preBCR 信号传导进行酪氨酸磷酸化。相应 cDNA 的克隆表明 pp36 是一种在 N 端区域进行脂质修饰的新型蛋白质。由于已知这种脂质修饰对于蛋白质锚定到脂筏中至关重要，因此 pp36 似乎在信号转导中发挥着重要作用。

项目成果

Nomura, K., Kanegane, H., Karasuyama, H., Tsukada, S., Agematsu, K., Murakami, G., Sakazume, S., Sako, M., Tanaka, R., Kuniya, Y., Komeno, T., Ishihara, S., Hayashi, K., Kishimoto, T.and Miyawaki, T.: "Genetic defect in human X-linked agammaglobulinemia i

野村，K.，兼金，H.，乌山，H.，冢田，S.，上松，K.，村上，G.，坂津，S.，佐子，M.，田中，R.，Kuniya，Y.，

Ohnishi, K., Shimizu, T., Karasuyama, H.and Melchers, F.: "The identification of a non-classical cadherin expressed during B cell development and its interaction with surrogate light chain."J.Biol.Chem.. 275. 31134-44 (2000)

Ohnishi, K.、Shimizu, T.、Karasuyama, H. 和 Melchers, F.：“B 细胞发育过程中表达的非经典钙粘蛋白的鉴定及其与替代轻链的相互作用。”J.Biol.Chem..

永田喜三郎: "Annual Review免疫 1999" 中外医学社, 1-13 (1998)

永田喜三郎：《免疫学年度评论 1999》《中外医学社》，1-13 (1998)

Kuramochi,S.: "Molecular cloning of the human gene STK10 encoding lymphocyte-oriented kinase and comparative chromosomal mapping of the human,mouse and rat homologues." Immunogenetics.

Kuramochi,S.：“编码淋巴细胞导向激酶的人类基因 STK10 的分子克隆以及人类、小鼠和大鼠同源物的比较染色体作图。”

烏山一: "B細胞初期分化とプレB細胞レセプターの発現"医学のあゆみ. 188. 202-203 (1999)

Kazuyama Karasuyama：“早期 B 细胞分化和前 B 细胞受体的表达”医学史 188. 202-203 (1999)。