Bone regeneration by gene activated matrix combined with nano-size calcium phosphate particle and minicircle DNA

基因激活基质结合纳米级磷酸钙颗粒和小环DNA进行骨再生

• 批准号: 24890167
• 负责人: MIURA Kei-ichiro
• 金额: $ 1.91万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-08-31 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24890167/
• 关键词: 骨再生; Gene Activated Matrix; 口腔外科; リン酸カルシウム; ナノサイエンス

By making use of minicircle DNA, this plasmid was sucsessfully made.And we found that cell transplantaiton efficiency of this plasmid was higher than conventional plasmid. After that we prepared minicircle DNA/calcium phosphate particle/collagen composite combined with Green Fluorescent Protein gene, and assessed the material in vivo analysis. During this period, we successfully made nano-submicro calcium phosphate particle and reported the osteoconduction of the material(K Miura,et al.Applied Surface Science.2013). Furthermore, we obtained the knowledge of bone regeneration material(A Matsui,et al.Cleft Palate-Craniofacl J,2013).

利用小环DNA，成功制备了该质粒。我们发现该质粒的细胞移植效率比常规质粒高。之后我们制备了结合绿色荧光蛋白基因的小环DNA/磷酸钙颗粒/胶原蛋白复合物，并对材料进行了体内分析。在此期间，我们成功制备了纳米亚微米级磷酸钙颗粒，并报道了该材料的骨传导性能(K Miura,et al.Applied Surface Science.2013)。此外，我们还获得了骨再生材料的知识（A Matsui,et al.Cleft Palate-Craniofacl J,2013）。

项目成果

The Regenerated Bone Quality by Implantation of Octacalcium Phosphate Collagen Composites in a Canine Alveolar Cleft Model

• DOI: 10.1597/12-096
• 发表时间: 2014-07-01
• 期刊: CLEFT PALATE-CRANIOFACIAL JOURNAL
• 作者: Matsui, Aritsune;Matsui, Keiko;Echigo, Seishi
• 通讯作者: Echigo, Seishi

Characterization and bioactivity of nano-submicro octacalcium phosphate/gelatin composite

• DOI: 10.1016/j.apsusc.2013.05.086
• 发表时间: 2013-10-01
• 期刊: APPLIED SURFACE SCIENCE
• 影响因子: 6.7
• 作者: Miura, Kei-ichiro;Anada, Takahisa;Suzuki, Osamu
• 通讯作者: Suzuki, Osamu

Weijian Zhong、朝比奈泉:骨髄濃縮液による骨組織再生の試み-BMP2との相乗効果の検討-

钟伟健、朝比奈泉：利用骨髓浓缩液再生骨组织的尝试 - 与BMP2的协同效应研究 -

• 发表时间: 2013
• 作者: 三浦桂一郎;住田吉慶;江頭寿洋;梅林真由美;長井一浩
• 通讯作者: 長井一浩