Tissue-selective heart failure treatment with the ischemic heart-homing peptide

使用缺血性心脏归巢肽治疗组织选择性心力衰竭

• 批准号: 23890230
• 负责人: KANKI Sachiko
• 金额: $ 2.08万
• 依托单位: Osaka Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23890230/
• 关键词: 心筋虚血; アフィニティ精製; ペプチド; アフィニティー精製; 心筋虚血再灌流傷害; ペプチド創薬

To find a receptor molecule of the ischemic heart-homing peptide, the affinity screening is necessary and the carrier must play a key role. FG beads, which are 200nm in diameter and consisted of magnetic ferrite covered by Poly-glycidylmethacrylate resin, are used as affinity carrier to bind the ischemic heart-homing peptides. The condition to bind the peptide to FG beads was optimized, and the combination of the acetylene peptide at N’-terminus and azide FG beads worked not destroy tertiary structure of peptide. Then, pull-down assay with the affinity beads was performed to find receptors for the homing peptide from ischemic heart tissue of rats, which is made by temporary occlusion of leftcoronary artery.

为了寻找缺血心脏归巢肽的受体分子，亲和力筛选是必要的，并且载体必须发挥关键作用，FG珠子由直径200nm、由聚甲基丙烯酸缩水甘油酯树脂覆盖的磁性铁氧体组成。结合缺血心脏归巢肽的亲和载体优化了肽与FG珠的结合条件，以及乙炔肽的结合。 N'端和叠氮化物FG珠不会破坏肽的三级结构。然后，用亲和珠进行pull-down测定，以从大鼠缺血性心脏组织中寻找归巢肽的受体，该组织是通过暂时闭塞左冠状动脉而制成的。动脉。