Production of 2-D nanocarbons with different bioaffinities as ROS quenching agents to improve mammalian oocyte cryopreservation outcomes

生产具有不同生物亲和力的二维纳米碳作为 ROS 猝灭剂，以改善哺乳动物卵母细胞冷冻保存结果

• 批准号: 22K20535
• 负责人: FERREPUJOL PILAR
• 金额: $ 1.83万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-08-31 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K20535/
• 关键词: graphene oxide; reduced graphene oxide; oocyte; in vitro maturation; cryopreservation; nanocarbons; reactive oxygen species

We prepared graphene oxide (GO) according to a previous method (Hummer’s method) and slowly performed thermal reduction at 80°C up to 2 weeks to ensure a successful reduction of the functional groups to obtain reduced graphene oxide (rGO). The GO and rGO samples were structurally analyzed by FTIR, TGA, XPS and XRD. To analyze their reactive oxygen species (ROS) activity, they were analyzed by ESR. Furthermore, to understand their ability to be used in a culture medium setting, the samples were submitted to a dispersion test.According to the results of all the tests, pristine GO (oxidation level 30-35%) was selected to analyze its toxicity during oocyte in vitro maturation (IVM) in the presence of non-enzymatic antioxidants. Results suggest that the addition of GO during oocyte IVM does not negatively affect oocyte viability significantly.

我们根据之前的方法（Hummer法）制备了氧化石墨烯（GO），并在80°C下缓慢进行热还原长达2周，以确保官能团成功还原，得到还原的氧化石墨烯（rGO）和。通过 FTIR、TGA、XPS 和 XRD 对 rGO 样品进行了结构分析。为了分析其活性氧 (ROS) 活性，还通过 ESR 对其进行了分析，以了解其在培养基环境中使用的能力。根据所有测试的结果，选择原始GO（氧化水平30-35%）来分析其在非酶抗氧化剂存在下的卵母细胞体外成熟（IVM）过程中的毒性。表明在卵母细胞 IVM 过程中添加 GO 不会对卵母细胞活力产生显着的负面影响。