Inhibition of RANK ligand-induced osteoclast differentiation by Porphyromonas gingivalis

牙龈卟啉单胞菌对 RANK 配体诱导的破骨细胞分化的抑制

• 批准号: 22890115
• 负责人: NAKAYAMA Masaaki
• 金额: $ 1.91万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22890115/
• 关键词: 歯周病原細菌; Porphyromonas gingivalis; 破骨細胞分化; 自然免疫; 抗酸菌; Toll-like receptor

In this study, we demonstrated whether P. gingivalis affects osteoclastogenesis induced by RANK/RANKL signaling. Osteoclastic precursor cells were challenged by P. gingivalis with RANKL at the same time, analyzed its differentiation for matured osteoclasts. Interestingly, the osteoclastic precursors were inhibited RANK/RANKL-induced osteoclastogenesis by infection of P. gingivalis. It was, however, contratory to my expectation that osteoclasts were promoted to form the large number of matured cells and activated bone resorption by P. gingivalis infection. According to investigate this inhibition by using precursor cells from knockout mice of TLRs and adaptor factors, MyD88 and TRIF, it revealed that innate immune response through TLR2/MyD88 signaling pathway were related with this inhibition.

在本研究中，我们证明了牙龈卟啉单胞菌是否影响 RANK/RANKL 信号传导诱导的破骨细胞生成。同时用RANKL攻击破骨细胞前体细胞，分析其向成熟破骨细胞的分化。有趣的是，破骨细胞前体通过感染牙龈卟啉单胞菌而抑制 RANK/RANKL 诱导的破骨细胞生成。然而，与我的预期相反的是，牙龈卟啉单胞菌感染促进破骨细胞形成大量成熟细胞并激活骨吸收。通过使用 TLR 和衔接因子 MyD88 和 TRIF 敲除小鼠的前体细胞研究这种抑制作用，发现通过 TLR2/MyD88 信号通路的先天免疫反应与这种抑制作用有关。

项目成果

歯周病原細菌Porphyromonas gingivalisによる宿主細胞応答の分子基盤

牙周病原菌牙龈卟啉单胞菌宿主细胞反应的分子基础

• 发表时间: 2011
• 作者: 奥野裕子;山本知加;辰巳愛香;吉崎亜里香;酒井佐枝子;毛利育子;谷池雅子;中山真彰
• 通讯作者: 中山真彰

The mechanism and the role of Akt dephosphorylation induced by Porphyromonas gingivalis infection

牙龈卟啉单胞菌感染诱导Akt去磷酸化的机制及作用

• 发表时间: 2012
• 作者: 中山真彰;藤田佑貴;井上哲圭;大原直也
• 通讯作者: 大原直也

Helicobacter pylori VacA Reduces the Cellular Expression of STAT3 and Pro-survival Bcl-2 Family Proteins, Bcl-2 and Bcl-XL, Leading to Apoptosis in Gastric Epithelial Cells

• DOI: 10.1007/s10620-010-1420-1
• 发表时间: 2011-04-01
• 期刊: DIGESTIVE DISEASES AND SCIENCES
• 影响因子: 3.1
• 作者: Matsumoto, Ayako;Isomoto, Hajime;Kohno, Shigeru
• 通讯作者: Kohno, Shigeru

MicroRNA signatures in Helicobacter pylori-infected gastric mucosa

• DOI: 10.1002/ijc.25348
• 发表时间: 2011-01-15
• 期刊: INTERNATIONAL JOURNAL OF CANCER
• 影响因子: 6.4
• 作者: Matsushima, Kayoko;Isomoto, Hajime;Kohno, Shigeru
• 通讯作者: Kohno, Shigeru

歯周病原細菌Porphyromonas gingivalisの病原性発現に関する新しい分子伝達システム

一种表达牙周病原菌牙龈卟啉单胞菌致病性的新型分子传递系统

• 发表时间: 2010
• 作者: 北野千鶴;奥野裕子;梶谷優貴;古藤雄大;永井利三郎;中山真彰
• 通讯作者: 中山真彰