Elucidating signaling components under low potassium conditions

阐明低钾条件下的信号成分

• 批准号: 21870045
• 负责人: SHIN Ryoung
• 金额: $ 1.76万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21870045/
• 关键词: カリウムトランスポーター; FOXライブラリ; 栄養欠乏; 非生物的ストレス; 転写因子

Arabidopsis FOX (Full-length cDNA over-expressor genes) agrobacterium librarys were transformed into established AtHAK5 promoter::luciferase Arabidopsis plants. These AtHAK5 promoter::luciferase plants with FOX library have been screened and more than 200 candidate transgenic plants that have higher luciferase activity under full nutrient condition have been isolated. Among them, fifteen lines were analyzed to determine which gene is overexpressed, and some candidates were analyzed for their expression in response to nutrient deficiency. The overexpression plants and knockout plants of WRKY TF were obtained. The overexpression plants showed increased tolerance to potassium deficient condition.

将拟南芥 FOX（全长 cDNA 过表达基因）农杆菌文库转化到已建立的 AtHAK5 启动子::荧光素酶拟南芥植物中。对这些带有FOX文库的AtHAK5启动子::荧光素酶植株进行了筛选，分离出200多株在全营养条件下具有较高荧光素酶活性的候选转基因植株。其中，对 15 个品系进行了分析以确定哪个基因过度表达，并分析了一些候选基因在营养缺乏时的表达情况。获得WRKY TF的过表达植株和敲除植株。过表达植物表现出对缺钾条件的耐受性增加。

项目成果

Dissection of Potassium Signaling in Arabidopsis thaliana

拟南芥钾信号传导的剖析

• 发表时间: 2010
• 作者: Minghao Zhang;門田康弘;申怜
• 通讯作者: 申怜

Identification of Arabidopsis potassium deficient signaling components via full-length cDNA overexpressor (FOX) gene hunting system and AtHAK5

通过全长 cDNA 过表达 (FOX) 基因狩猎系统和 AtHAK5 鉴定拟南芥钾缺乏信号成分

• 发表时间: 2009
• 作者: 高久誉大;滝沢由政;辻田幹;石田恭子;両角佑一;胡桃坂仁志;申怜
• 通讯作者: 申怜