Genome-wide microsatellite mapping of multi-factorial ocular diseases.

多因素眼部疾病的全基因组微卫星图谱。

基本信息

批准号：
16209052
负责人：
MIZUKI Nobuhisa
金额：
$ 30.28万
依托单位：
Yokohama City Univercity
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16209052/
关键词：
microsatellite gene mapping High myopia Behcet disease Lattice degeneration Hypertension SNP genome medical science 緑内障

项目摘要

In order to identify susceptibility genes associated with ocular diseases and hypertension, we have performed association study, using 25000 microsatellites(MS) and pooled DNA. The progresses of each disease is as follows.High myopia : 534 samples were collected. After the first, second and third screen, 156 positive markers were passed. As a result of indivisual typing, 26 positive markers were obtained. SNP typings were performing in the surrounding each candidate regionBehcet disease : 427 samples were collected. The first, second and third MS screenings and individual genotyping were finished, and 11 positive markers were obtained. SNP typing are performing in the surrounding each candidate regions.Lattice degeneration : 397 samples were collected. In the first screening, we found significant association for 2851 markers.Hypertension : 508 samples were collected. MS screenings were finished and 135 positive markers were obtained. Individual typing then were performed, and 55 markers were passed. SNP genotyping are performing now.Glaucoma : 192 samples were collected. The first screen using 138 samples was performed and 13.8% positive markers were obtained. The second screen is performing now. Simultaneously, SNP typings using GeneChip are performing.

为了鉴定与眼部疾病和高血压相关的易感基因，我们使用了25000个微卫星（MS）和合并的DNA进行了关联研究。每种疾病的进展如下。近视高：收集了534个样本。在第一个，第二和第三屏之后，通过了156个正标记。由于不独特的键入，获得了26个正标记。 SNP打字在周围的每个候选区域疾病中进行：收集427个样本。第一，第二和第三MS筛选以及单个基因分型完成，并获得了11个正标记。 SNP打字在周围的每个候选区域都进行。局部变性：收集了397个样品。在第一次筛选中，我们发现了2851个标记的显着关联。高血压：508个样本。完成了MS筛选，并获得了135个正标记。然后执行单个键入，并通过55个标记。 SNP基因分型现在正在执行。Glaucoma：收集了192个样品。使用138个样品进行了第一个屏幕，并获得了13.8％的阳性标记。第二个屏幕正在执行。同时，使用Genechip的SNP键入正在执行。

项目成果

期刊论文数量（123）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Single nucleotide polymorphisms of Ficolin 2 gene in Behcet's disease

DOI：
10.1016/j.jdermsci.2006.05.010
发表时间：
2006-09-01
期刊：
JOURNAL OF DERMATOLOGICAL SCIENCE
影响因子：
4.6
作者：
Chen, Xixue;Katoh, Yasunobu;Mizuki, Nobuhisa
通讯作者：
Mizuki, Nobuhisa

HLA検査.基礎からわかるぶどう膜炎

HLA检测。葡萄膜炎的基本认识

DOI：
发表时间：
2006
期刊：
影响因子：
0
作者：
Yoshida K;Kase S;Nakayama K;Nagahama H;Harada T;Ikeda H;Harada C;Imaki J;Ohgami K;Shiratori K;Iliyana B I;Ohno S;Nishi S;Nakayama K I;佐々木爽
通讯作者：
佐々木爽

The study of investigation of susceptibility genes of Behcet disease using Genome-wide microsatellite marker.

利用全基因组微卫星标记调查白塞病易感基因的研究。

DOI：
发表时间：
2004
期刊：
The study report of Behcet disease in 2006, The enterprise for the intractable disease, In Japan Ministry of Health
影响因子：
0
作者：
出村昌史(金沢大学臓器機能制御学);生水真紀夫;木啓一;神崎晋;武田仁勇;Bulun Serdar;Yoshizawa S;Mizuki N
通讯作者：
Mizuki N

Exclusion of the transforming growth factor-β as a candidate gene for high myopia in the Japanese.

排除转化生长因子-β作为日本人高度近视的候选基因。