Oxalate metabolism in hemodialysis patients

血液透析患者的草酸代谢

基本信息

批准号：
16209048
负责人：
OGAWA Yoshihide
金额：
$ 31.62万
依托单位：
University of the Ryukyus
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16209048/
关键词：
oxalate metabolism serum oxalate renal failure oxalosis hyperoxaluria vitamin C hemodialysis patients vitamin B6 deficiency グリコール酸エリスロポエチンビタミンB6 シュウ酸カルシウム

项目摘要

Oxalate is a uremic toxin, that can be deposited in the tissues and organs of long-term hemodialysis patients. Arteriosclerosis develops early in these patients and is associated with a high risk of hypertension, coronary artery disease, and cerebrovascular disease. Hyperoxalemia damages the vascular endothelium, giving rise to arteriosclerosis. Patients also suffer from carboxyl stress because of their uremic state, and administration of ascorbate may improve this redox condition and may also be beneficial for various vicious cycles, e.g., that leading to cancer. High doses of ascorbate, however, cause hyperoxalemia in dialysis patients and potentially lead to the onset of oxalosis.1.Capillary electrophoresis for measurement of serum oxalate was first introduced to our laboratory about 6 years ago. Measurement of serum oxalate-related substances in 452 hemodialysis patients showed that the serum oxalate level was strongly associated with serum ascorbate, suggesting that ascorbate inta … More ke may influence serum oxalate. Vitamin C administration improves iron utilization and may be useful for erythropoietin-refractory anemia in dialysis patients. It has also been suggested that vitamin C administration may reduce the erythropoietin dose. To balance the benefit and risks, the does of vitamin C should be limited to around 100 mg per day. Calcium phosphate is deposited in the tissues of dialysis patients, who may also suffer from calcium oxalate deposition when the serum oxalate level exceeds 100 μmol/l (unstable supersaturation). Hyperoxalemia above this level is often seen in patients with primary hyperoxaluria. When bone histology was studied along with serum oxalate-related substances before and after parathyroidectomy, the serum oxalate level was not correlated with any of the parameters assessed.2.Alanine-glyoxylate aminotransferase (AGT) is one of glyoxylate detoxifying enzyme localized in the peroxisome of hepatocytes that handle glyoxylate converted from glycolate. Glyoxylate reductase is localized in hepatocytes and other tissues, and it minimizes oxalate production from glyoxylate. Deficiency of vitamin B6, a cofactor of AGT, causes oxalosis and hemodialysis patients tend to be deficient in water -soluble vitamins. Oxalate precursors (glyoxylate, glycolate, hydroxyproline, hydroxypyruvate, xylitol, etc.) augment oxalate production in vitamin B6-deficient rats. Dialysis patients should avoid a diet containing oxalate precursors and such dietary restriction appears to be useful in treating hyperoxalemia.3.Capillary electrophoresis combined with mass spectrometry improves the sensitivity of measuring serum oxalate by several-fold, enabling the serum levels of oxalate and glycolate to be easily determined. Using this method, we can confirm peaks that were previously thought to be oxalate precursors. Repeated measurement of serum oxalate allows us to get some new ideas for a highly sensitive oxalate assay, which will eventually be developed. These are the fruits obtained by the financial support of the Japanese Ministry of Education, Culture, Sports, Science, and Technology.In conclusion, we acknowledge the financial support for our research on oxalate in dialysis patients and hope that the results obtained in our experiments may be beneficial for the management of dialysis patients in the near future. This is not the end of our research, but instead represents a stepping stone. We are very eager to continue the same research and we hope for continuing support. Less

草酸盐是一种尿毒症毒素，可以沉积在长期血液透析患者的组织和器官中。这些患者的动脉硬化早期发展，与高血压，冠状动脉疾病和脑血管疾病的高风险有关。高氧血症会损害血管内皮，从而导致动脉硬化。患者还因其尿毒症状态而患有羧基应激，并且抗坏血酸盐可能会改善这种氧化还原状况，并且可能对导致癌症的各种恶性周期有益。然而，高剂量的抗坏血酸会导致透析患者的高清血症，并有可能导致草氧发作。1。毛细血管电泳，用于测量血清草酸酸盐的毛细血管，大约6年前首次引入了我们的实验室。 452例血液透析患者血清草酸盐相关物质的测量表明，血清草酸盐水平与血清抗坏血酸血清密切相关，这表明抗坏血酸酯会影响……更多的KE可能影响血清草酸盐。维生素C的给药可改善铁的利用率，可能对透析患者的红细胞生成蛋白 - 难治性贫血有用。还有人提出，维生素C的给药可以减少促红细胞生成素剂量。为了平衡利益和风险，DO DO VITAMIN C每天应限制为100毫克。磷酸钙沉积在透析患者的组织中，当血清草酸盐水平超过100μmol/L时，他们也可能患有草酸钙沉积（不稳定的过饱和）。在原发性高氧确实尿症的患者中，通常可以看到高于该水平的高血压。当骨组织学与草酸血清相关物质进行研究之前和之后，血清草酸酯水平与评估的任何参数无关。2.ALANINE---甘氧基氨基转移酶（AGT）是甘酰胺在乙酰盐酸盐中替代了含氧化氧化氧化物的含氧化含量，使得源自氧化氧化物氧化氧化物。糖酸。乙二醇还原位于肝细胞和其他组织中，并最大程度地减少了从乙二基产生的草酸盐产生。维生素B6（AGT的辅助因子）的缺乏会导致草氧病和血液透析患者缺乏水 - 溶质维生素。草酸盐前体（乙二醇，乙醇酸酯，羟基丙烯，羟基丙酮酸，木糖醇等）增强了维生素B6缺乏大鼠的草酸盐产生。透析患者应避免饮食中含有草酸盐前体的饮食，而这种饮食限制似乎在治疗高氧血症中很有用。3.CAPILLARY电泳与质谱量相结合，可以提高测量血清Oxalate的灵敏度，从而可以轻松确定Oxalate和glycaly的串行水平。使用这种方法，我们可以确认以前认为是草酸盐前体的峰。重复测量血清草酸盐使我们能够获得一些新的想法，以进行高度敏感的草酸盐评估，最终将开发出来。这些是由日本教育，文化，体育，科学和技术的财政支持获得的果实。总之，我们承认我们对透析患者中草酸盐研究的财务支持，并希望我们实验中获得的结果可能对在近期近来的透析患者的管理可能有益。这不是我们研究的终结，而是代表垫脚石。我们非常渴望继续进行同样的研究，我们希望继续提供支持。较少的

项目成果

期刊论文数量（188）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Urinary response to an oxalic acid load is influenced by the timing of calcium loading in rats.

DOI：
10.1097/01.ju.0000176457.34601.d7
发表时间：
2005-11
期刊：
The Journal of urology
影响因子：
0
作者：
R. Hossain;Y. Ogawa;M. Morozumi;S. Hokama;A. Uchida;K. Sugaya
通讯作者：
R. Hossain;Y. Ogawa;M. Morozumi;S. Hokama;A. Uchida;K. Sugaya

Relationship between serum ascorbate and oxalate levels in dialysis patients given vitamin C.

接受维生素 C 的透析患者血清抗坏血酸和草酸水平之间的关系。

DOI：
发表时间：
2004
期刊：
Abstract of the 13rd Annual Meeting of Kidney and EPO research conference. 30
影响因子：
0
作者：
Fukano;Asami;Shigeko;Haruyama;Ogawa Y.
通讯作者：
Ogawa Y.

Major factors modulating the serum oxalic acid level in hemodialvsis patients

血液透析患者血清草酸水平的主要调节因素

DOI：
发表时间：
2004
期刊：
Frontiers in Bioscience 9
影响因子：
0
作者：
Sato;T.;Saito;R.;Jinushi;T.;Tsuji;T.;Matsuzaki;J.;Koda;T.;Nishimura;S.;Takeshima;H.;Nishimura;T.;Yoshihide Ogawa
通讯作者：
Yoshihide Ogawa

Diagnosis and classification of renal osteodystrophy.

肾性骨营养不良的诊断和分类。