The analysis of ERECTA gene, which is responsible for the elongation of the stem, in Arabidopsis.

拟南芥中负责茎伸长的 ERECTA 基因分析。

• 批准号: 15207001
• 负责人: KOMEDA Yoshibumi
• 金额: $ 30.87万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15207001/
• 关键词: Arabidopsis; stem; elongation; erecta mutants; floral stem; 花序形態; 茎伸長; ERECTA遺伝子; ERBP遺伝子; 頂端分裂組織

The aim of this study is: to understand the processes during the development of higher plants especially for the elongation of the floral stems in Arabidopsis. For that, our tools are to isolate mutants and to analyze the responsible genes.1.The study of two genes for erecta-binding proteins.The erbpl and 2 double mutants expressed seedling-lethality. The ontogenic defective point was searched by observation of the embryos from globular stage to maturity. The defect revealed after the onset of the heart-stage-embryo. The relation to auxin was determined by using auxin-reporter DR5-uidA.2.To search new factors for the regulation of ERECTA gene.We collected many mutants which have reduced expression of ER gene by using ER-uidA(from E. coli) reporter. We isolated mutants carrying reduced expression of erecta (as rex) 1 and 2 genes. Their responsible genes encode for pectin-synthesis and for factors for cellar organella, respectively. The regulation of related genes were determined under the rexl and 2 background to know the regulation.

这项研究的目的是：了解较高植物的发展过程中的过程，尤其是在拟南芥中花茎的伸长率。为此，我们的工具是分离突变体并分析负责的基因。1。对勃起结合蛋白的两个基因的研究。ERBPL和2个双突变体表示幼苗的杀伤性。通过观察从球体阶段到成熟的胚胎来搜索个体中的缺陷点。心脏阶段 - 永远贝发作后揭示的缺陷。与生长素的关系是通过使用生长素 - 培养剂Dr5-uida.2来确定的。我们分离了携带勃起表达降低的突变体（AS REX）1和2基因。他们的负责基因分别编码果胶合成和地窖有机埃拉的因素。相关基因的调控是在REXL和2个背景下确定的，以了解调节。

项目成果

Annual review of plant biology

• DOI: 10.1146/arplant.682
• 发表时间: 2002
• 作者: D. Delmer;H. Bohnert;S. Merchant;W. Gruissem

The dwarf phenotype of the Arabidopsis acl5 mutant is suppressed by a mutation in an upstream ORF of a bHLH gene

• DOI: 10.1242/dev.02535
• 发表时间: 2006-09-15
• 期刊: DEVELOPMENT
• 影响因子: 4.6
• 作者: Imai, Akihiro;Hanzawa, Yoshie;Takahashi, Taku
• 通讯作者: Takahashi, Taku

Abe, M., Katsumata, H., Komeda, Y., Takahashi, T.: "Regulation of shoot epidermal cell differentiation by a pair of homeodomain proteins in Arabidopsis."Development. 130. 635-643 (2003)

Abe，M.，Katsumata，H.，Komeda，Y.，Takahashi，T.：“拟南芥中一对同源结构域蛋白对芽表皮细胞分化的调节。”开发。

The plant exon finder : a tool for precise detection of exons using a T-DNA-based tagging approach.

植物外显子查找器：使用基于 T-DNA 的标记方法精确检测外显子的工具。

• 发表时间: 2004
• 期刊: Gene 338
• 作者: Jingu F;Shirase T;Ohtomo I;Imai A;Komda Y;Takahashi T
• 通讯作者: Takahashi T

組換えDNA実験技術と植物遺伝学

重组DNA实验技术和植物遗传学

• 发表时间: 2006
• 期刊: 生物の科学・遺伝 60・1
• 作者: Okamoto;M.;Kuwahara A.;Seo;M.;Kushiro;T.;Asami;T.;Hirai;N.;Kamiya Y.;Koshiba;T.;Nambara;E.;米田好文
• 通讯作者: 米田好文