Function of fos family genes in the differentiation of chicken chondrocytes

fos家族基因在鸡软骨细胞分化中的作用

基本信息

批准号：
06680677
负责人：
IBA Hideo
金额：
$ 1.41万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680677/
关键词：
chondrocytes AP-1 fos jun chicken embryos retrovirus vectors Transcription Factor limb buds アルカリ性フォスファターゼ

项目摘要

In this study, we have analyzed the expression levels of Fos/Jun family proteins (AP-1) and the biological effects of ectopic expression of each fos/jun family genes in the cultured chondrocytes or in limb buds of early chicken embryo.1.We have established a methodology to introduce and express exogenous genes in the entire limb region of chicken embryo (day 1.5) by microinjecting avian replication-competent retrovirus vectors. The ectopic expression of all the known fos or jun family genes of chicken (c-fos, fra-2, c-jun or junD) in the limb buds caused no changes in the pattern formation of bones or in the morphology. When the v-fos gene of FBJ-MuSV was introduced, however, all the long bones such as tibia, fibula and femur were shortened significantly (40-70% of the uninfected counterpart). Pathological analysis indicated that v-fos virus infected chondrocytes have assumed the morphology of proliferating chondrocytes and the differentiation into mature or hypertropic chondrocytes were drastically inhibited.2.We have analyzed the expression levels of Fos or Jun family proteins in the cultured chondrocytes deribed from both lower and upper region of steruna (LS and US,respectively). The most clear difference observed between the two types of chondrocytes were the expression levels in c-Jun, LS,which is in more immature stage, expresses higher levels of c-Jun. And ectopic expression of c-Jun in US cells caused stimulation of cellular growth and inhibition of differentiation, indicating the importance of c-Jun function in the chondrocyte differentiation.

在这项研究中，我们分析了每个FOS/JUN家族基因在培养的软骨细胞中或早期鸡肉胚胎的肢体中，每个FOS/JUN家族基因的异位表达的表达水平以及每个FOS/JUN家族基因的异位表达的生物学作用。我们已经建立了一种方法，可以通过微注射鸟类复制逆转录病毒载体在鸡胚胎（第1.5天）中引入和表达外源基因（第1.5天）。肢体芽中所有已知的FOS或JUN家族基因的异位表达（C-Fos，Fra-2，C-Jun或Jund）的异位表达不会导致骨骼的模式形成或形态学的变化。然而，当引入FBJ-MUSV的V-FOS基因时，明显缩短了所有长骨，例如胫骨，腓骨和股骨（未感染的40-70％）。病理分析表明，VOS病毒感染的软骨细胞已经假定了增殖软骨细胞的形态，并且分化为成熟或肥大软骨细胞的分化得到了极大抑制。2。我们已经分析了来自培养的软骨细胞中Fos或Jun家族蛋白的表达水平。 Steruna的下部和上部区域（分别是LS和美国）。两种类型的软骨细胞之间观察到的最明显的差异是C-Jun，LS中的表达水平，在更不成熟的阶段，表达了较高水平的C-JUN。 C-JUN在美国细胞中的异位表达导致细胞生长刺激和分化的抑制，这表明C-JUN功能在软骨细胞分化中的重要性。