STUDIES ON A BLOOD-GROUP DEPENDENT GLYCOPROTEIN ASSOCIATED SPECIFICALLY WITH HUMAN INTESTINAL ALKALINE PHOSPHATASE

与人肠道碱性磷酸酶特异性相关的血型依赖性糖蛋白的研究

• 批准号: 06672302
• 负责人: KOMODA Tsugikazu
• 金额: $ 1.34万
• 依托单位: SAITAMA MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06672302/
• 关键词: ALKALINE PHOSPHATASE; HIGH-MOLECULAR WIGHT INTESTINAL AP; BLOOD GROUP DEPENDENT; BINDING GLYCOPROTEIN; MOLECULAR SIZE; BLOOD GROUPS B OR O; LEWIS SECRETARY GROUP; B-TRANSFERASE; 小腸アルカリ性ホスファーゼ; O-グリコシド結合型糖鎖; 絶食時; 特異的結合タンパク; 精製法; Triton X-100

ABNORMALLY MIGRATING ALKALINE PHOSPHATASES (AP_2) ARE DETECTED MORE FREQUENTRY BY POLYACRYLAMIDE GEL DISC ELECTROPHORESIS (PAGE) THAN WITH CELLULOSE ACETATE MEMBRANE,A HIGH-MOLECULAR WEIGHT INTESTINAL AP (HIAP) DETECTED IN HEALTHY SERUM AND ASSOCIATED WITH THE ABH AND LEWIS BLOOD GROUPS WAS INVESTIGATED.BY PREINCUBATION OF BLOOD GROUP B OR O SERA AND A MONOCLONAL ANTIBODY TO HUMAN INTESTINAL AP,THE HIAP WAS FOUND AT THE ORIGIN OF PAGE.IT WAS ABLE TO NORMALIZE HIAP BY BRIEF TREATMENT WITH SUBTILISIN OR PAPAIN.THE RESULTING AP WAS EXHIBITED TO DETECT THE SAME MOBILITY AS COMMON INTESTINAL AP.BLOOD APPEARANCE OF HIAP WAS SPECIFICALLY DEPENDED ON HEALTHY BLOOD GROUP O AND B SUBJECTS BEARING WITH LEWIS b BLOOD GROUP (SECRETARY TYPE). WHEN THE TESTED SUBJECTS BEARING LEWIS a BLOOD GROUP (NON-SECRETARY TYPE) WAS USED,THERE IS LITTLE IF ANY DETECTED THE HIAP IN THEIR CIRCULATIONS.BASED ON THE RESULTS OF GEL FILTRATION,THE MOLECULAR SIZE OF THE GLYCOPROTEIN BINDING TO INTESTINAL AP HAD APPROXIMATELY 30 KILODALTONS WITHOUT THE INTESTINAL AP AND A DETERGENT.HOWEVER,THE GLYCOPROTEIN BOUND TO INTESTINAL AP IN THE PRESENCE OF TRITONX-100 WAS FOUND AT THE SAME MOBILITY AS THE HIAP IN BLOOD GROUP O AND B SUBJECTS BEARING LEWIS b BLOOD GROUP.FURTHERMORE,IT IS INTERESTING THAT CIRCULATING AMOUNTS OF THE GLYCOPROTEIN BINDING TO INTESTINAL AP ARE MATCHED APPARENTLY TO THE BLOOD GROUP B OR O-TRANSFERASE.TAKEN TOGETHER,THE BLOOD APPEARRANCE OF INTESTINAL AP IN HUMAN SUBJECTS MAY BE RELATED STRONGLY TO THE BLOOD GROUPS ABH AND LEWIS.

聚丙烯酰胺凝胶盘电泳（页）比醋酸纤维素膜更频繁地检测到异常迁移的碱性磷酸酶 (AP_2)，这是一种在健康血清中检测到的高分子量肠 AP (HIAP)，与对ABH和Lewis血型进行了研究。通过将B型或O型血型血清与人肠AP单克隆抗体预孵育，在Page的起源处发现了HIAP。通过枯草杆菌蛋白酶或木瓜蛋白酶的短暂治疗能够使HIAP正常化。结果AP 的检测与普通肠 AP 的移动性相同。HIAP 的血液外观具体取决于具有 LEWIS b 血型（秘书型）的健康 O 型和 B 型受试者。当使用刘易斯血型（非分泌型）的测试对象时，其循环中几乎没有检测到HIAP。根据凝胶过滤的结果，与肠AP结合的糖蛋白的分子大小没有肠 AP 和洗涤剂时约为 30 千道尔顿。然而，在 TRITONX-100 存在下，发现与肠 AP 结合的糖蛋白具有与携带 LEWIS b 血的 O 型和 B 型血型受试者中的 HIAP 相同的迁移率此外，有趣的是，与肠 AP 结合的糖蛋白的循环量显然与 B 型血或 O 型转移酶相匹配。总而言之，人类受试者中肠 AP 的血液外观可能与血型 ABH 和刘易斯。

项目成果

Yamagishi,F.,Komoda,T.,Alpers,D. H. et al: "Clearance of surfactant-like particle proteins from circulation in rats" American Journal of Physiology. 266. G596-G605 (1994)

山岸 F.、小莫田 T.、阿尔珀斯 D.

KOYAMA,I., SATO,M., SAKAI,H., NAGATA,A., MIURA,M., SAKAGISHI,Y., KOMODA.T.: "CARBOHYDRATE-MEDIATED RECOGNITION OF A CIRCULATING PLACENTAL ALKALINE PHOSPHATASE-IMMUNOGLOBULIN M COMPLEX" CLINICA CHIMICA ACTA. 230. 9-19 (1994)

小山，I.，佐藤，M.，坂井，H.，永田，A.，三浦，M.，坂岸，Y.，KOMODA.T.：“碳水化合物介导的循环胎盘碱性磷酸酶-免疫球蛋白 M 复合物的识别

Arai,K., Sumi,S., Yoshida,K., Komoda,T.: "A precursor from of human kidney γ-glutamyl transferase in normal and cancer tissues, and its post-translational modification" Biochimica et Biophysica Acta. 1253. 33-38 (1995)

Arai, K.、Sumi, S.、Yoshida, K.、Komoda, T.：“正常组织和癌组织中人肾 γ-谷氨酰转移酶的前体及其翻译后修饰”Biochimica et Biophysicala Acta 1253。 .33-38 (1995)

菰田二一,他: "小腸様ALPの構造と臨床応用" 生物物理化学. 38. 167-171 (1994)

Niichi Komoda 等：“小肠样 ALP 的结构和临床应用”生物物理化学 38. 167-171 (1994)。

KOBAYASHI,F., IKEDA,T., TOZUKA,S., NOGUCHI,O., FUKUMA,T., SAKAMOTO,S., MARUMO,F., KOMODA.T., SAKAGISHI,Y., SATO,C.: "A VARIANT ALKALINE PHOSPHATASE FOUND IN A CASE OF GASTRIC CARCINOMA WITH SUPER BONE SCAN" GUT. 36. 299-302 (1995)

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