STUDIES ON A BLOOD-GROUP DEPENDENT GLYCOPROTEIN ASSOCIATED SPECIFICALLY WITH HUMAN INTESTINAL ALKALINE PHOSPHATASE

与人肠道碱性磷酸酶特异性相关的血型依赖性糖蛋白的研究

• 批准号: 06672302
• 负责人: KOMODA Tsugikazu
• 金额: $ 1.34万
• 依托单位: SAITAMA MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06672302/
• 关键词: ALKALINE PHOSPHATASE; HIGH-MOLECULAR WIGHT INTESTINAL AP; BLOOD GROUP DEPENDENT; BINDING GLYCOPROTEIN; MOLECULAR SIZE; BLOOD GROUPS B OR O; LEWIS SECRETARY GROUP; B-TRANSFERASE; 小腸アルカリ性ホスファーゼ; O-グリコシド結合型糖鎖; 絶食時; 特異的結合タンパク; 精製法; Triton X-100

ABNORMALLY MIGRATING ALKALINE PHOSPHATASES (AP_2) ARE DETECTED MORE FREQUENTRY BY POLYACRYLAMIDE GEL DISC ELECTROPHORESIS (PAGE) THAN WITH CELLULOSE ACETATE MEMBRANE,A HIGH-MOLECULAR WEIGHT INTESTINAL AP (HIAP) DETECTED IN HEALTHY SERUM AND ASSOCIATED WITH THE ABH AND LEWIS BLOOD GROUPS WAS INVESTIGATED.BY PREINCUBATION OF BLOOD GROUP B OR O SERA AND A MONOCLONAL ANTIBODY TO HUMAN INTESTINAL AP,THE HIAP WAS FOUND AT THE ORIGIN OF PAGE.IT WAS ABLE TO NORMALIZE HIAP BY BRIEF TREATMENT WITH SUBTILISIN OR PAPAIN.THE RESULTING AP WAS EXHIBITED TO DETECT THE SAME MOBILITY AS COMMON INTESTINAL AP.BLOOD APPEARANCE OF HIAP WAS SPECIFICALLY DEPENDED ON HEALTHY BLOOD GROUP O AND B SUBJECTS BEARING WITH LEWIS b BLOOD GROUP (SECRETARY TYPE). WHEN THE TESTED SUBJECTS BEARING LEWIS a BLOOD GROUP (NON-SECRETARY TYPE) WAS USED,THERE IS LITTLE IF ANY DETECTED THE HIAP IN THEIR CIRCULATIONS.BASED ON THE RESULTS OF GEL FILTRATION,THE MOLECULAR SIZE OF THE GLYCOPROTEIN BINDING TO INTESTINAL AP HAD APPROXIMATELY 30 KILODALTONS WITHOUT THE INTESTINAL AP AND A DETERGENT.HOWEVER,THE GLYCOPROTEIN BOUND TO INTESTINAL AP IN THE PRESENCE OF TRITONX-100 WAS FOUND AT THE SAME MOBILITY AS THE HIAP IN BLOOD GROUP O AND B SUBJECTS BEARING LEWIS b BLOOD GROUP.FURTHERMORE,IT IS INTERESTING THAT CIRCULATING AMOUNTS OF THE GLYCOPROTEIN BINDING TO INTESTINAL AP ARE MATCHED APPARENTLY TO THE BLOOD GROUP B OR O-TRANSFERASE.TAKEN TOGETHER,THE BLOOD APPEARRANCE OF INTESTINAL AP IN HUMAN SUBJECTS MAY BE RELATED STRONGLY TO THE BLOOD GROUPS ABH AND LEWIS.

通过聚丙烯酰胺凝胶盘电泳（PAGE）比异常迁移的碱性磷酸酶（AP_2）比与乙酸纤维素膜相比，乙酸纤维素膜（一种高分子肠道AP（HIAP）在健康的血清中检测到的高分子体重AP（HIAP），并与ABH和Lewis Blood群体相关。在Page的起源处发现了肠道AP，它可以通过用枯草蛋白酶或木瓜蛋白酶进行简短处理来使HIAP归一化。所得的AP显示出与HIAP的常见肠道AP.Blood出现相同的迁移率，它特别依赖于健康的血液组O和B受试者Blood Blood Group（秘书类型）。当使用了刘易斯一组的测试受试者（非秘书类型）时，几乎没有检测到的循环中的HIAP。基于凝胶过滤的结果，糖蛋白的分子大小与肠道AP结合的分子尺寸在没有肠内的AP和DETERENTERTINAL的情况下，与肠内的限制大约是ggly的。发现Tritonx-100与持有刘易斯B血型的血液组和B受试者的HIAP相同。

项目成果

Yamagishi,F.,Komoda,T.,Alpers,D. H. et al: "Clearance of surfactant-like particle proteins from circulation in rats" American Journal of Physiology. 266. G596-G605 (1994)

山岸 F.、小莫田 T.、阿尔珀斯 D.

KOYAMA,I., SATO,M., SAKAI,H., NAGATA,A., MIURA,M., SAKAGISHI,Y., KOMODA.T.: "CARBOHYDRATE-MEDIATED RECOGNITION OF A CIRCULATING PLACENTAL ALKALINE PHOSPHATASE-IMMUNOGLOBULIN M COMPLEX" CLINICA CHIMICA ACTA. 230. 9-19 (1994)

小山，I.，佐藤，M.，坂井，H.，永田，A.，三浦，M.，坂岸，Y.，KOMODA.T.：“碳水化合物介导的循环胎盘碱性磷酸酶-免疫球蛋白 M 复合物的识别

Arai,K., Sumi,S., Yoshida,K., Komoda,T.: "A precursor from of human kidney γ-glutamyl transferase in normal and cancer tissues, and its post-translational modification" Biochimica et Biophysica Acta. 1253. 33-38 (1995)

Arai, K.、Sumi, S.、Yoshida, K.、Komoda, T.：“正常组织和癌组织中人肾 γ-谷氨酰转移酶的前体及其翻译后修饰”Biochimica et Biophysicala Acta 1253。 .33-38 (1995)

菰田二一,他: "小腸様ALPの構造と臨床応用" 生物物理化学. 38. 167-171 (1994)

Niichi Komoda 等：“小肠样 ALP 的结构和临床应用”生物物理化学 38. 167-171 (1994)。

KOBAYASHI,F., IKEDA,T., TOZUKA,S., NOGUCHI,O., FUKUMA,T., SAKAMOTO,S., MARUMO,F., KOMODA.T., SAKAGISHI,Y., SATO,C.: "A VARIANT ALKALINE PHOSPHATASE FOUND IN A CASE OF GASTRIC CARCINOMA WITH SUPER BONE SCAN" GUT. 36. 299-302 (1995)

小林F.、池田T.、户冢S.、野口O.、福间T.、坂本S.、丸毛F.、小MODA.T.、坂岸Y.、佐藤C.：