Experimental study of protective of in-situ hypothermic liver perfusion on the ischemic liver with biliary obstruction in pigs

原位低温肝灌注对猪缺血性肝胆道梗阻保护作用的实验研究

基本信息

批准号：
06671280
负责人：
ASAHARA Toshimasa
金额：
$ 1.34万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

To minimize the damage during total hepatic vascular exclusion and after reperfusion, in-situ cold perfusion via portal vein and hepatic artery was carried out in pig for clinical application to liver surgery of hepatocellular carcinoma and cholangiocellular carcinoma with liver cirhosis and obstructive jaundice.1. Study of the usefulness of V-V bypass and cold perfusion of the liverIn-situ hypothermic perfusion could maintain hepatic TAN level higher than that of warm ischemia group during and sfter HVE (hepatic vascular exclusion). In-situ hypothermic perfusion could prevent the damage caused by liver ischemia.2. Study of the usefulness of V-V bypass and cold perfusion of the liver with obstructive jaundiceMore remarkable liver cell injury was induced by warm ischemia in pig with obstructive jaundice, and in-situ hypothermic perfusion of the liver could prevent the damages during and after liver ischemia not only in normal liver but also in the liver with obstructive jaundice.3. Study for the possibility of prologation of V-V bypass and cold perfusoin of the liverThe in-situ hypothermic perfusion technique is useful for reducing ischemia-reperfusion injury, and especially could protect the liver injury from 180 minutes ischemia of the liver with UW solution.4. Study for the protective effect of PGI2 analogue (OP2507) on liver ischemic injuryPGI2 analogue has a cytoprotective effect and maintains high energy metabolism of the liver against ischemic reperfusion injury. Administration of PGI2 analogue with in-situ liver cold perfusion could prevent the damage caused by prolonged liver ischemia.

为了最大程度地减少肝血管排斥期间的损害，再灌注后，通过门静脉和肝动脉进行了原位冷灌注，在PIG中进行了临床应用，以临床应用于肝细胞癌和胆管细胞癌的肝脏手术，并用肝cir虫和阻塞性鼻鼻涕的肝癌。研究V-V旁路的有用性和肝素 - 阳性低温灌注的冷灌注可以使肝棕褐色水平高于和SFTER HVE（肝血管排除）高于温暖的缺血组。原位低温灌注可以防止肝缺血造成的损害2。研究V-V旁路和肝脏冷灌注的有用性是通过阻塞性肝细胞损伤引起的显着性肝细胞损伤，这是通过阻塞性黄疸的猪中的温暖缺血引起的，肝脏的肝脏灌注可能会在正常肝脏中不仅在正常肝脏中，而且还可以防止肝脏损害在正常的肝脏中，而且还可以防止肝脏中的肝脏造成的损害。研究可能会在肝脏的V-V旁路和冷饮食中进行原位的低温灌注技术的可能性，可用于减少缺血 - 再灌注损伤，尤其可以通过UW溶液从肝脏的180分钟内保护肝损伤。4。 PGI2类似物（OP2507）对肝脏缺血性损伤类似物的保护作用的研究具有细胞保护作用，并保持肝脏对缺血性再灌注损伤的高能量代谢。用原位肝冷灌注对PGI2类似物的施用可以防止长时间的肝脏缺血造成的损害。