Expression of a brain/embryo-type myosin heavy chain isoform (MIIB2) in chronic glomerulonephritis, especially IgA glomerulonephritis

脑/胚胎型肌球蛋白重链异构体 (MIIB2) 在慢性肾小球肾炎，特别是 IgA 肾小球肾炎中的表达

• 批准号: 06671145
• 负责人: MAEDA Takao
• 金额: $ 1.41万
• 依托单位: Wakayama Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671145/
• 关键词: mesangial proliferative glomerulonephritis; IgA glomerulonephritis; myosin isoforms; anti-Thy 1.1 nephritis

Proliferation of mesangial cells is frequently found in glomerulonephritis such as IgA glomerulonephritis. Recent reports suggest that a brain/embryo-type myosin isoform (MIIB2) is involved in cell proliferation. We have studied the expression of MIIB2 in renal biopsy samples from patients with various renal diseases and in the renal tissues from the rat model of mesangial proliferative glomerulonephritis induced with anti-Thy 1.1 antibody. Immunohistochemical analysis of the biopsy samples using an anti-brain-type myosin heavy chain-specific monoclonal antibody (HBM1) indicated that 92% of the samples from patients with IgA glomerulonephritis contained a significant population of mesangial cells that reacted with the antibody. Most of sample from patients with other types of proliferative glomerular diseases also contained HBM1-reactive mesangial cells, while none of the samples from patients with non-proliferative glomerular diseases contained a significant population of HBM1-reactive mesangial cells. The anti-Thy 1.1-injected rats began to express an increased level of a brain/embryo-type myosin heavy chain isoform (MIIB2) in the mesangial cells at 5 days after injection of the antibody and continued to express the isoform up to the 10th day. On the other hand, the expression of the proliferating cell unclear antigen in the mesangial cells was induced at 3 days after injection of anti-Thy 1.1 antibody and was maximal at the 4th day. The expression of type I collagen, a major extracellular matrix component of the glomeruli, was induced about 5 days after injection of the anti-Thy 1.1 antibody. These results indicate that the expression of the MIIB2 isoform by mesangial cells is accelerated in proliferative glomerulonephritis and suggest that the myosin isoform is involved in the phenotypic transformations of the glomerular tissues rather than in the cell proliferation.

在肾小球肾炎（例如IgA肾小球肾炎）中经常发现肾小球细胞的增殖。最近的报道表明，脑/胚胎型肌球蛋白同工型（MIIB2）参与细胞增殖。我们已经研究了来自各种肾脏疾病患者的肾脏活检样品中MIIB2的表达，以及用抗THY 1.1抗体诱导的肾小球增殖性肾小球肾炎的大鼠模型的肾脏组织中的表达。使用抗脑型肌球蛋白重链特异性单克隆抗体（HBM1）对活检样品进行免疫组织化学分析（HBM1）表明，来自IgA肾小球肾炎患者的92％的样品含有与抗体反应的大量中膜细胞。来自其他类型的增生性肾小球疾病患者的大多数样本也包含HBM1反应性膜细胞，而来自非增强性肾小球疾病患者的样本均未包含大量HBM1反应性膜细胞。注射抗体后5天，抗肌肉1.1注射的大鼠开始表达脑/胚胎型肌球蛋白重链同工型（MIIB2）的水平，并继续表达同工型，直到第10天。另一方面，在注射抗Thy 1.1抗体后3天诱导了增殖细胞在膜细胞中的表达不清的抗原，并且在第四天最大。注射抗Thy 1.1抗体后约5天，诱导了肾小球的主要细胞外基质成分的I型胶原蛋白的表达。这些结果表明，在增殖性肾小球肾炎中加速了MESANGIAL细胞MIIB2同工型的表达，并表明肌球蛋白同工型与肾小球组织的表型转化有关，而不是在细胞增殖中。

项目成果

KISHINO M,KIMURA A,YAMAGUCHI K,OHTANI H,YAMADA Y,TAKAHASHI T,MUNE M,MIMURA K,MAEDA T,MATSUMURA S,TSUJI T,YUKAWA S: "Increased expression of a brain/embryo-type myosin heavy chain isoform (MIIB2) in mesangial proliferative glomerulonephritis" Kidney Int. 5

KISHINO M、KIMURA A、YAMAGUCHI K、OHTANI H、YAMADA Y、TAKAHASHI T、MUNE M、MIMURA K、MAEDA T、MATSUMURA​​ S、TSUJI T、YUKAWA S：“脑/胚胎型肌球蛋白重链亚型的表达增加

Kishino M et al: "Increased expression of a brain/embryo-type myosin heavy chain isoform (MIIB2) in mesangial proliferative glomerulonephritis" Kidney International. 50. 1350-1359 (1996)

Kishino M 等人：“系膜增生性肾小球肾炎中脑/胚胎型肌球蛋白重链异构体 (MIIB2) 的表达增加”肾脏国际。

Masarori K・sh・no,et al: "Increased expression of a brair/embryo-tgpe myosin heavy chain isoform(MIIB2)in mesangial proliferative jlomerulonaphritis" Kidney International. 49(in press). (1996)

Masarori K. sh. no 等人：“系膜增生性肾小球肾炎中 brair/胚胎-tgpe 肌球蛋白重链亚型 (MIIB2) 的表达增加”《肾脏国际》49（出版中）。