Role of Ca-activated K channel and Na pump in the regulation of airway smmoth muscle tone.

钙激活钾通道和钠泵在气道平滑肌张力调节中的作用。

• 批准号: 06670632
• 负责人: TAMAOKI Jun
• 金额: $ 1.09万
• 依托单位: Tokyo Women's Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670632/
• 关键词: Airway smooth muscle; Ion channel; Patch clamp; cyclic nucleotide; asthma; Bronchodilator; アセチルコリン; 気道過敏性

1. Muscle contractile and relaxant responsesIsolated bronchial segments were mounted in organ chambers and the contractile responses to acetylcholine and the relaxant responses to various bronchodilating agents were determined in the presence and absence of K channel blockers and ouabain. Acetylcholine-induced contrction was potentiated by charybdotoxin and ouabain, and the relaxant responses to salbutamol, forskolin and nitroprusside were attenuated by these drugs, whereas glibenclamide and apamin had no effect. Thus, Ca-activated K channel and Na pump play roles in the regulation of airway smooth muscle tone by depolarizing membrane potential.2. Accumulation of cyclic nucleotidesIntracellular cyclic nucleotide concentrations were determined in the presence and absence of charybdotoxin and ouabain. Salbutamol and forskolin increased cAMP conctents and nitroprusside increased cGMP contents, and these effects were not altered by charybdotoxin and ouabain, indicating that intracellular cAMP and cGMP directly stimulate Ca-activated K channel and Na pump in airway smooth muscle cells.3. Patch clamp studiesAddition of DBcAMP and DBcGMP each caused single K channel ion current. Therefore, it was confirmed that these cyclic nucleotides produce Ca-activated K channel opening and Na-K-ATPase activation.

1。在器官室中安装了肌肉收缩和松弛反应性的支气管段，并在存在和不存在K通道阻滞剂和Ouabain的情况下确定了对乙酰胆碱的收缩反应，并确定对各种支气管扩张剂的松弛反应。乙酰胆碱诱导的原状由Charybdotoxin和Ouabain增强，这些药物对Salbutamol，Forskolin和硝化剂的松弛反应受到这些药物的减弱，而Glibenclamide和Apamin则没有影响。因此，通过去极化膜电位，CA激活的K通道和NA泵在调节气道平滑肌张力中起着作用。2。在存在和不存在Charybdotoxin和Ouabain的情况下确定环状核苷酸核苷酸循环核苷酸浓度的积累。 Salbutamol和Forskolin增加了营地的cAMP征收，硝化剂增加了CGMP的含量，并且Charybdotoxin和ouabain不会改变这些作用，表明细胞内营地和CGMP直接刺激CA激活的CA激活的K通道，而NA泵的NA Pump在气道平滑肌细胞中。3。 DBCAMP和DBCGMP的斑块夹研究导致单个K通道离子电流。因此，证实这些环核苷酸产生了CA激活的K通道开口和Na-K-ATPase激活。

项目成果

E.Tagaya: "Regulation of airway Cholinergic neurotrausmission by Ca^<2+>-activated K chaunel and Na^+-K^+-a denosine tri phosphatase" Experimeutal Long Research. 21. 683-694 (1995)

E.Tagaya：“Ca ^ 2 -激活的K通道和Na ^ -K ^ -腺苷三磷酸酶调节气道胆碱能神经损伤”实验长期研究。

E.Tagaya et al.: "Regulation of airway cholinergic neurotransmission by Ca^<2+>-activated K^<> channel and Na^+-K^+ adenosinetriphosphatase" Experimental Lung Research. 21. 683-694 (1995)

E.Tagaya等人：“Ca^2-激活的K^<>通道和Na^-K^腺苷三磷酸酶调节气道胆碱能神经传递”实验肺研究。

玉置 淳: "気道平滑筋とカルシウムイオン" Clinical Calcium. 5. 753-757 (1995)

Jun Tamaki：“气道平滑肌和钙离子”临床钙。5. 753-757 (1995)

E. Tagaya: "Regnlation of airway cholinergic neurotransmission by Ca^<2+>-activated K^+channel and Na^+-K^+ adenosine triphosphatase" Experimental Lung Research. 21. 683-694 (1995)

E. Tagaya：“Ca^2-激活的K^通道和Na^-K^三磷酸腺苷对气道胆碱能神经传递的调节”实验肺研究。

J.Tamaoki: "Role of K^+ channel opening and Na^+-K^+ATPase activity in airway relaxation induced by solbutamol" Life Sciences. 55. PL217-223 (1994)

J.Tamaoki：“K^ 通道开放和 Na^ -K^ ATP 酶活性在 solbutamol 诱导的气道松弛中的作用”生命科学。