Interrelationship between growth factor and adhesion molecule in the formation of neuromuscular junctions and muscle apindles

生长因子和粘附分子在神经肌肉接头和肌轴形成中的相互关系

• 批准号: 06670025
• 负责人: DESAKI Junzo
• 金额: $ 1.15万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670025/
• 关键词: neuromuscular junction; muscle spindle; motor ending; sensory ending; basic fibroblast growth factor; neural cell adhesion molecule; immunoelectron microscopy; 神経筋接合部(NMJ); 知覚神経終末; 塩基性線維芽細胞成長因子(bFGF); 神経性細胞接着因子(N-CAM)

The morphodifferentiation of neuromuscular junctions and muscle spindles in the postnatal rats and Chinese hamsters was observed by ordinary electron microscopy and the interrelationship between basic fibroblast growth factor (bFGF) and neural cell adhesion molecule (N-CAM) during their formation and maturation was examined by immuno-electron microscopy.Motor endings at birth were polyneuronal and subneural apparatuses consisted of shallow cup-like depressions with a few incipient junctional folds. Poly-neuronal motor endings gradually eliminated until the 15th postnatal day and only a motor ending retained. Synaptic gutters became complex and contained increased junctional folds. On the other hand, in muscle spindles at birth, intrafusal myotubes established side-to-side contact with each other without the intercalation of a basal lamina. Sensory endings were confined to the outer surface of the muscle bundle. Thereafter, intrafusal myotubes separated into independent muscle fibers which were innervated by sensory endings and ensheathed in attenuated inner capsule cells.In the examination by immunoelectron microscopy, positive staining for bFGF was observed in both motor and sensory endings until adulthood. Moreover, fibroblasts among muscle fibers and outer and inner capsules of the muscle spindles were also bFGF-positive. On the other hand, N-CAM was detected at motor ending-muscle fiber contacts, but was not necessarily observed at sensory ending-intrafusal muscle fiber contacts.

普通的电子显微镜以及在其形成和成熟的质子上检查了碱性细胞粘附分子（N-CAM），通过普通电子显微镜（BFGF）和神经细胞粘附分子（N-CAM）在其形成和成熟的情况下，通过免疫释放量检查，通过普通电子显微镜（BFGF）在其形成和成熟的基本细胞粘附分子（N-CAM）之间观察到了产后大鼠和中国仓鼠中神经肌肉连接和肌肉纺锤的形态分化。多神经元和神经下设备由浅杯状凹陷组成，并具有一些初期的连接褶皱。多神经元电动机末端逐渐消除，直到产后第15天，并且只保留了电动机结束。突触沟变得复杂，并包含增加的连接褶皱。另一方面，在出生时肌肉纺锤体中，恒星内肌管彼此之间建立了侧面接触，而没有基础层层的插入。感觉结尾局限于肌肉束的外表面。此后，肉间内肌管分为独立的肌肉纤维，这些肌肉纤维被感觉末端支配并在衰减的内囊细胞中被包含。在通过免疫电子显微镜检查的检查中，在运动和感觉终结末至ad植物的运动中都观察到BFGF的阳性染色。此外，肌肉纺锤体的肌肉纤维以及外部和内部胶囊之间的成纤维细胞也是BFGF阳性的。另一方面，在运动结束肌肉纤维触点上检测到N-CAM，但不一定在感觉结束肌肉纤维接触中观察到。