Molecular approaches to the pathogenesis of glomerulanephritis by vasoactive substances

血管活性物质对肾小球肾炎发病机制的分子研究

基本信息

批准号：
05837007
负责人：
TERADA Yoshio
金额：
$ 1.09万
依托单位：
Tokyo Medical & Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

项目摘要

Renal nephron segments were heterogeneous, and receptors for endothelin (ET)-1, ET-3, Angiotensin II (AT II), EGF,and IGF-1 distribute differently along the nephron segments. We have revealed that the PCR localizatoin of two types (V2, V1) of vasopressin receptor, and angiotensin II receptor. We also reported that localization of mRNA and protein of ET-3 along the nephron segments. Recently, growth factors and vasoactive substances are reported to stimulate mitogen-activated protein kinase (MAP-K). In this study, we showed that mRNA of MEK-K,Raf-1-K,MAPK-K,MAP-Ks(p42 and p44), and S6-K was expressed in nephron segments using RT-PCR technique. We showed that MAP-K cascades are expressed in every nephron segment. ET-1, ET-3, AT II,EGF,and IGF-I stimulate MAP-K cascades heterogeneously along the nephron segment. We concluded that MAP-K cascades play an important role in the regulation of renal function. We also reported that the presence of C-type natriuretic factor and its receptor (GC-B) along the nephron. The CNP system may play an important roles in modulatiing the salt and body fluid homeostasis and blood pressure. We also analyzed the expression of PDGF and PDGF-receptor expression in the glomeruli of IgA nephropathy patients. Thus, we investigated the new approaches to the pathogenesis of glomerulonephritis and renal functions.

肾脏肾单位段是异质的，内皮素（ET）-1，ET-3，血管紧张素II（在II），EGF和IGF-1的受体沿着肾单位段分布不同。我们已经揭示了加压素受体和血管紧张素II受体的两种类型（V2，V1）的PCR局部生节蛋白。我们还报道了沿肾单位段的ET-3的mRNA和蛋白质的定位。最近，据报道生长因子和血管活性物质刺激有丝分裂原激活的蛋白激酶（MAP-K）。在这项研究中，我们表明使用RT-PCR技术在肾单位段中表达了MEK-K，RAF-1-K，MAPK-K，MAP-K，MAP-K，MAP-K，MAP-K，MAP-K，MAP-K，MAP-K，MAP-K，MAP-K，MAPK-K，MAPK-K，MAPK-K，MAPK-K，MAPK-K，MAPK-K，MAPK-K，MAPK-K，MAPK-K（P42和P44）的mRNA。我们表明，MAP-K级联反应在每个肾单位段中表达。 ET-1，ET-3，AT II，EGF和IGF-I沿肾单段异质地刺激MAP-K级联反应。我们得出的结论是，MAP-K Cascades在肾功能的调节中起着重要作用。我们还报道了沿肾单位的C型纳餐地及其受体（GC-B）的存在。 CNP系统可能在调节盐和体液稳态和血压方面起着重要作用。我们还分析了IgA肾病患者肾小球中PDGF和PDGF受体表达的表达。因此，我们研究了肾小球肾炎和肾功能发病机理的新方法。