Dectection of leukemia-associate antioncogene located on the long-arm of human chromosome 9.

位于人类9号染色体长臂上的白血病相关抑癌基因的检测。

• 批准号: 04671520
• 负责人: TANIMOTO Mitsune
• 金额: $ 1.22万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671520/
• 关键词: Human leukemia; Molecular biology; Gene deletion; Tumor suppressor gene; Chromosome 9; Chromosome defect; 染色体欠欠; 遺伝子欠失; 遺伝子クローニング

We have analyzed more than 200 patients with acute myeloid leukemia in adults by chromosome analysis and found that long arm of chromosome 9 was deleted interstitially in 4 patients. From these patients, normal lymphoblastoid cell line was established and defected gene was analyzed by polymorphic DNA probes. Among 4 patients, chromosome region ranging from 9p21 to 9d32 was deleted. Further study using polymerase chain reaction on several loci of chromosome 9 revealed that exact gene deletion involves aldorase B locus in one patient. Pin-point search of chromosome 9 showed that anti-cancer gene associated with these myeloid leukemia patients may present near D9S176 and/or D9S180 locus. Further characterization of the missing gene is now underway.

我们已经通过染色体分析分析了成人的200多名急性髓样白血病患者，发现9例染色体的长臂在4例患者中被肺部删除。从这些患者中，建立了正常的淋巴细胞细胞系，并通过多态性DNA探针分析了缺陷的基因。在4例患者中，删除了从9p21到9d32的染色体区域。在9号染色体的几个基因座上使用聚合酶链反应的进一步研究表明，确切的基因缺失涉及一名患者的醛固酶B基因座。对9染色体的PIN点搜索表明，与这些髓样白血病患者相关的抗癌基因可能在D9S176和/或D9S180基因座附近存在。现在正在进行进一步的丢失基因表征。

项目成果

Matsuoka A.: "Unexpected Heterogeneity of PML/RARα Fused mRNA Detected by Nested Polymerase Chain Reaction in Acute Promyelocytic Leukemia" Leukemia. 7. 1151-1155 (1993)

Matsuoka A.：“急性早幼粒细胞白血病中嵌套聚合酶链反应检测到的 PML/RARα 融合 mRNA 的意外异质性”白血病。7. 1151-1155 (1993)

Miyamura K.: "Long Persistent bcr-abl Positive Transcript by Polymerase Chain Reaction After Marrow Transplant to Chronic Leukemia" Blood. 81. 1089-1093 (1993)

Miyamura K.：“骨髓移植慢性白血病后聚合酶链反应显示长期持续的 bcr-abl 阳性转录本”血液。

K.Miyaura,M.Tanimoto el al: "Dctection of Philadelphia Chromosome-positive Acute Lymphoblastic Leukemia by Polymerase Chain Reaction: Possible eradication of Minimai Residual Disease by Trausplantation" Blood. 79. 1366-1370 (1992)

K.Miyaura、M.Tanimoto 等人：“通过聚合酶链反应检测费城染色体阳性急性淋巴细胞白血病：通过移植可能根除微小残留病”血液。

Miyamura K.: "Long persistent bcr-abl positive transcript by polymerase chain reaction after marrow transplant for chronic myelogenous leukemia without clinical relapse : A study of 64 patients." Blood. 81. 1089-1093 (1993)

Miyamura K.：“慢性粒细胞白血病骨髓移植后聚合酶链反应显示长期持续的 bcr-abl 阳性转录本，且无临床复发：一项针对 64 名患者的研究。”

Miyamura K.: "Detection of Philadelphia Chromosomc-Positive Acute Lymphoblastic Leukemia by Polymerase Chain Reaction" Blood. 79. 1366-1370 (1992)

Miyamura K.：“通过聚合酶链反应检测费城染色体阳性急性淋巴细胞白血病”血液。