MRS analysis of Tumors in the maxillo-facial region

颌面部肿瘤的MRS分析

• 批准号: 04671219
• 负责人: ITO Jusuke
• 金额: $ 1.41万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671219/
• 关键词: 31p-MR spectroscopy; 31P-MRS; experimental tumors; maxillofacial region; MRS; 顎顔面領域腫瘍; ハムスター頬粘膜腫瘍

In vivo 31P-MR spectroscopy was performed on experimental cancers transplantedin animals. 31P-MR spectra of tumors transplanted in the submucosal tissue of the buccal pouch of hamsters were obtained using of a JEOL JMN-GX270 spectrometer with a JEOL NM-G27TSPW surface coil probe. Tumors transplanted were derived from cultured tissues obtained from lymphatic metastatic foci from cancers induced with DMBA in the buccal pouch of hamsters. Following parameters were evaluated. 1) PME/total, 2) Pi/total, 3) PDE/total, 4) PCr/total, 5) ATP/total, 6) PCr/Pi, 7) PCr/ATP,8) PME/ATP,9) PDE/ATP,10)PDE/PME,11) PME/Pi, 12) PDE/Pi, 13) Pi/ATP,14) pHi. Results : In none of 31MR spectroscopy on the buccal pouch mucous membrane of 10 hamsters as the control group, any significant peaks were obtained. In the tumorgroup (12 tumors) ATP (alpha, beta, gamma), PCr, Pi, PME,PDE peaks were obtained. PCr/total, PCr/Pi, PCr/ATP ratios decreased as tumors increaed in size whereas PDE/total ratio increased in proportion to increase in tumors size. There was no sig-nificant correlation between the tumor size and other parameters.Discussion : PCr definitely decreased with incease in tumor size. This indicated that tumors were evolving into the hypoxic state with tumor growth. There was nosignificant correlation between tumor growth and pHi. This suggested that tumorswere not in the anaerobic state with necrosis but rather in the state where the accumulation of lactic acid as an anaerobic metabolite was not enough to cause areduction in a pH.Increase in PDE suggested an acceleration in membrane metabo-lism. However, in this study the degree of resolution between PDE and PME was not sufficiently sharp to indicate accurate mambrane metabolism.conclusions : In 31MR spectroscopy of the normal contro group there were no significant peaks obtained. In the tumor group ATP,PCr, Pi, PME,PDE peaks were obtained. PCr/total, PCr/Pi, PCr/ATP ratioes decreased with tumor growth.

对移植到动物体内的实验性癌症进行了体内 31P-MR 光谱分析。使用带有JEOL NM-G27TSPW表面线圈探针的JEOL JMN-GX270光谱仪获得仓鼠颊囊粘膜下组织中移植的肿瘤的31P-MR光谱。移植的肿瘤来源于仓鼠颊囊中用 DMBA 诱导的癌症的淋巴转移灶获得的培养组织。评估以下参数。 1) PME/总计, 2) Pi/总计, 3) PDE/总计, 4) PCr/总计, 5) ATP/总计, 6) PCr/Pi, 7) PCr/ATP,8) PME/ATP,9) PDE/ATP,10)PDE/PME,11)PME/Pi,12)PDE/Pi,13)Pi/ATP,14)pHi。结果：作为对照组的10只仓鼠颊囊粘膜的31MR波谱均未出现显着峰。在肿瘤组（12个肿瘤）中获得了ATP（α、β、γ）、PCr、Pi、PME、PDE峰。 PCr/total、PCr/Pi、PCr/ATP 比率随着肿瘤大小的增加而降低，而 PDE/total 比率随着肿瘤大小的增加而成比例增加。肿瘤大小与其他参数无显着相关性。讨论：随着肿瘤大小的增加，PCr明显下降。这表明肿瘤随着肿瘤的生长而逐渐进入缺氧状态。肿瘤生长与pHi之间没有显着相关性。这表明肿瘤并非处于坏死的厌氧状态，而是处于厌氧代谢物乳酸积累不足以引起pH降低的状态。PDE的增加表明膜代谢加速。然而，在本研究中，PDE 和 PME 之间的分辨率不够清晰，不足以指示准确的膜代谢。结论：在正常对照组的 31MR 光谱中，没有获得显着的峰。肿瘤组获得ATP、PCr、Pi、PME、PDE峰。 PCr/total、PCr/Pi、PCr/ATP 比率随着肿瘤生长而降低。