Functional and morphological analysis of the main excretory duct IV.

主排泄管的功能和形态分析 IV．

• 批准号: 04671115
• 负责人: SATO Atsuko
• 金额: $ 1.34万
• 依托单位: Fukuoka Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671115/
• 关键词: Tuft cell; Main excretory duct; Submandibular gland; Glycoconjugate cytochemistry; Lectins; ruthenium red; Postnatal development; Male rat; ルテニユ-ム レッド; Submandibular gland; Main excretory duct; Tuft cells; postnatal development; Rat(Wister st

The tuft cells found in the main excretory duct epithelium of the submandibular gland are also present in the hollow organs of endodermal orgin such as the respiratory systems and the alimentary systems. Their common chracteristics are the finger-like long microvilli, microtubules and microfilaments running parallel to the cell axis, and a lot of vesicles and tubules in the apical cytoplasm. However, their functions are still unknown. The following experiments were done to establish their functions1)Vesicles and tubules contained the filamentous substance. Glycoconjugate cytochemistry were performed to prove the character of the filamentous substance. Vesicles, tubules Golgi elements and plasma membrane of the microvilli stained positive.2)The tuft cells were studied by embedding water soluble resin LRW and postembedded staining with lectin/ colloidal-gold (CG) conjugate-Con-A,PNA,SBA,UEA-1, DBA.Con-A was observed in the vesicles and tubules in the apical cytoplasm.3)The postnatal development of tuft cells at various stages, 0,1,7,14,21,28 and 54 days, was investigated by scanning and transmission electron microscopy. Tuft cells were already recognized at birth. Their number reached approximately 6% of the total epithelial cells. The frequency of tuft cells in the adult stage was about 7%. However, microvilli were smaller than the adult ones. In fine structure, the tuft cells at 28 days after birth reached the same level as that at birth.ConclusionThe tuft cells in the main excretory duct epithelium of the rat submandibular gland seem to engage in secretory activity of glycoconjugates, which may be related to the reception because of long microvilli protruding into the lumen.

下颌下腺主排泄管上皮中发现的簇状细胞也存在于内胚层起源的中空器官中，例如呼吸系统和消化系统。其共同特征是具有与细胞轴平行的手指状长微绒毛、微管和微丝，顶端细胞质内有大量囊泡和小管。然而，它们的功能仍然未知。进行了以下实验来确定它们的功能1）囊泡和小管含有丝状物质。进行糖缀合物细胞化学来证明丝状物质的特性。囊泡、小管高尔基体和微绒毛质膜染色呈阳性。2)通过包埋水溶性树脂LRW并用凝集素/胶体金(CG)缀合物-Con-A、PNA、SBA、UEA进行包埋后研究簇细胞-1, 在顶端细胞质的囊泡和小管中观察到DBA.Con-A。3) 簇绒的出生后发育通过扫描和透射电子显微镜研究不同阶段（0、1、7、14、21、28和54天）的细胞。簇细胞在出生时就已被识别。它们的数量约占上皮细胞总数的6%。成体阶段簇细胞的频率约为7%。然而，微绒毛比成人的要小。在精细结构上，出生后28天的簇状细胞已达到出生时的水平。结论大鼠颌下腺主排泄管上皮簇状细胞似乎从事复合糖的分泌活动，这可能与由于长微绒毛伸入管腔而导致接收。

项目成果

Sato, A.: "Fine structure of the tuft cells in the main excretory duct of the rat submandibular gland." Cell Tissue Res. (in press). (1995)

Sato, A.：“大鼠颌下腺主要排泄管簇细胞的精细结构。”

Sato, A.: "Postnatal development of the tuft cells of the rat submandibular gland." Anatomical Record. (in press). (1995)

Sato, A.：“大鼠颌下腺簇细胞的产后发育。”

Sato,A.: "Fine structure of The tuft cells inthe main excretory duct of the rat submandibular gland." Cell Tissue Res. (in press). (1995)

Sato,A.：“大鼠颌下腺主要排泄管簇细胞的精细结构。”

Atsuko SATO: "Fine structure and subepithelial capillaries of the main excretory duct epithelium of the submandibular gland of the japanese monkey,Macaca fuscara." Anatomical Record. (1993)

Atsuko SATO：“日本猴颌下腺主排泄管上皮的精细结构和上皮下毛细血管。”

Sato,A.: "Postnatal development of the tuft cells of the rat submandibular gland." Anatomical Record. (in press). (1995)

Sato,A.：“大鼠颌下腺簇细胞的产后发育。”