STUDY OF SIGNAL FACTORS ON DIMORPHISM OF CANDIDA ALBICANS

白色念珠菌二态性信号因子的研究

• 批准号: 04671116
• 负责人: CHO Tamaki
• 金额: $ 1.22万
• 依托单位: FUKUOKA DENTAL COLLEGE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04671116/
• 关键词: Opportunistic fungus; Candida albicans; Budding growth; Mycelial growth; Signal factors; Starvation; Dimorphism; Peptide like substances; 形態分化因子; 生物活性ペプチド

We extracted from Candida albicans JCM 9061 a fraction which was active in inducing germ tube formation in the same strain using the extraction methods for biologically active peptides described by Kangawa et al.(1983). Extraction by acid was performed on cells under five different conditiones : mid-exponential phase cells, starved exponential phase cells, starved exponential phase cells with germ tubes, stationary phase cells, and starved stationary phase cells. The active fraction was obtained by gel-filtration of extracts from cells in all five conditions. The fraction had a relative molecular mass in the 1,000 to 2,000 range. When the active fraction at the concentration of 3.8 mug protein/ml was added to zinc medium and starved exponential phase cells were cultured in it, around 70% of cells formed germ tubes after 60 min of incubation. The fractions ability to induce germination was lost by treatment with pronase. We hypothesize that the fraction contains a peptide-like substance which is active in inducing germ tube formation.

我们从白色念珠菌JCM 9061中提取了一种分数，该部分使用Kangawa等人（1983）描述的生物活性肽的提取方法在相同的菌株中诱导胚胎管形成。在五个不同条件下的细胞上进行酸提取：指指指数的中期细胞，饥饿的指数相细胞，用胚芽管，固定相细胞和饥饿的固定相相细胞饥饿的指数相细胞。通过在所有五个条件下的细胞提取物中提取物的凝胶过滤获得了活性分数。该馏分的相对分子质量在1,000至2,000范围内。当将3.8 mug蛋白/mL浓度下的活性分数添加到锌培养基中，并在其中培养饥饿的指数相细胞时，在孵育60分钟后，大约70％的细胞形成的细菌管。通过使用prinase的治疗失去了诱导发芽的分数能力。我们假设该馏分包含一种活跃于诱导细菌管形成的肽样物质。