The effects of local factors on bone remodeling

局部因素对骨重建的影响

• 批准号: 62480371
• 负责人: KUMEGAWA Masayoshi
• 金额: $ 4.16万
• 依托单位: Meikai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480371/
• 关键词: Osteoblasts; Osteoclasts; 局所因子; 骨茅細胞

We established a new culture system for osteoclast formation from 5-fluorouracil-treated mouse spleen cells unsupported by osteoblastic cells (Kurihara et al. Blood in press). Using this system we examined the effects of factors or osteoblastic cells on osteoblastic cell formation. The results obtained were as follows. Vitamin D_3, parathyroid hormone, glucorticoids. Prostaglandin (PG) F_<2 >, IL-1( ) and SGF stimulated osteoclast formation, whereas TGF- , PGE_1 and PGE_2 inhibited the formation (Sumitani et al. Endocrinology in preparation). Osteoblastic cells were involved in osteoclast formation: undifferentiated osteoblastic cells inhibited the formation. While differentiated ones stimulated it (Hiura et al. Endocrinology in prerparation). Osteoblastic celis mediated the action of TGF- and vitamin D_3 to the precursors for osteoclasts. IL-1( ) acted also MC3T3-El cells (Ikeda et al. Calcif. Tissue Int.).

我们建立了一种新的培养系统，用于从5-氟尿嘧啶处理的小鼠脾细胞中形成破骨细胞的新培养系统，该细胞不受成骨细胞的支持（Kurihara等人的血液中的血液）。使用该系统，我们检查了因子或成骨细胞对成骨细胞形成的影响。获得的结果如下。维生素D_3，甲状旁腺激素，糖尿类药物。前列腺素（PG）F_ <2>，IL-1（）和SGF刺激了破骨细胞的形成，而TGF-，PGE_1和PGE_2抑制了形成（Sumitani等人的内分泌学制备）。成骨细胞参与破骨细胞形成：未分化的成骨细胞抑制了形成。而差异化刺激了它（Hiura等人。成骨细胞Celis介导了TGF-和维生素D_3的作用，以使其前体的前体进行破骨细胞。 IL-1（）也起作用MC3T3-EL细胞（Ikeda等人Calcif。Stransueint。）。

项目成果

Kenji,Hiura; Koji,Sumitani; Terushige,Kawata; Kiyoshi,Higashino; Eiko,Kurihara; Takuya,Sato; Yoshiyuki,Hakeda; Masayoahi,Kumegawa: "Role of Mouse Osteoblastic Cells (MC3T3-E1) on Osteoblastic Cell Formation" Endocrinology.

健二、日浦；

N.Kurihara,;T.Suda,;Y.Miura,H.;Kodama,;M.Kumegawa,: Bloos in press.

N.Kurihara,;T.Suda,;Y.Miura,H.;Kodama,;M.Kumekawa,：布卢斯正在出版。

Y.Hakeda;T.Hotta;N.Kurihara;E.lkeda;N.Maeda;Y.Yagyu.M.Kumegawa: Endocrinology. 121. 1966-1974 (1987)

Y.Hakeda；T.Hotta；N.Kurihara；E.lkeda；N.Maeda；Y.Yagyu.M.Kumekawa：内分泌学。

Seiichi.Ishizuka;Noriyoshi.Kurihata;Shigeyasu.Hakeda;Norihiko.Maeda;Katshumi.Ikeda;Masayoshi.Kumegawa;Anthony W.Norman: Endocrinology.

Seiichi.Ishizuka；Noriyoshi.Kurihata；Shigeyasu.Hakeda；Norihiko.Maeda；Katsumi.Ikeda；Masayoshi.Kumekawa；Anthony W.Norman：内分泌学。

Eiko,Ikeda; Masami,Kusaka; Yoshiyuki,Hakeda; Kazushige,Yokota; Masayoshi,Kumegawa; Shozo,Yamamoto: "Effects of Interleukin 1 Beta on Osteoblastic MC3T3-E1 Cells" Calcif. Tissue int.43. 162-166 (1988)

池田英子；