Studies on the mechanism for salt tolerance in the cultured cells selected from tropical crops

热带作物培养细胞耐盐机制的研究

基本信息

批准号：
62480051
负责人：
YASUDA Takeshi
金额：
$ 3.58万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-62480051/
关键词：
Tropical plants Cultured cells Salt tolerance Halophyte Proline Betaine プロリンベタイン水ストレス

项目摘要

Whole plant and cultured cells of rice varieties, Nona Bokra and Chiem Chanh, were tested for the response to high NaCl conditions. Nana Bokra, that relatively stronger in salt tolerance than Cliem Chanh, showed lower Na and higher K contents than Chiem Chanh. Cultured cells from halophytic plants(Atriplex species) were also grew well under high salt condition. These cells maintained high K content, and betaine levels increase under high NaCl conditions. Salt tolerant phragmites communis accumulated proline and betaine as the compatible solute. These indicates that salt tolerant plants are also salt tolerant in cell levels.Selected salt tolerant(ST) cells from sugarcane and tobacco showed different reacts to salt conditions. Sugarcane sT cells maintained K, Mg and Ca contents in cultured on NaCl medium. On the other hand, tobacco ST cells showed low contents of K, Mg and Ca at high salt condition, but grew well. These indicate that two ST cells have different mechanisms of salt tolerance. As the osmotic regulators sugarcance accumulated proline, and tobacco accumulated betaine, but these content were lower than in halophyte cells.Protoplast preparation and cultivation were tested for the purpose to introduce the salt tolerant abilities to tropical crops. Cultured cells from several halophytic plants were treated different enzyme combinations. Pant hormone combinations and cell proliferating abilities affected to prepare clean protoplasts. Protoplasts from atriplex species were applied to protoplast fusion with tobacco mesophyll protoplasts.

测试了水稻品种的整个植物和培养细胞，Nona Bokra和Chiem Chanh，是否对高NaCl条件的反应进行了测试。娜娜·博克拉（Nana Bokra）的盐耐受性比克里姆·陈（Cliem Chanh）相对较强，比chiem chanh的Na含量较低，K含量更高。来自盐生植物（阿特里普物种）的培养细胞在高盐状态下也生长得很好。这些细胞保持较高的K含量，在高NACL条件下，甜菜碱水平升高。盐耐受性稀疏smumcomis群积聚了脯氨酸和甜菜碱作为兼容的溶质。这些表明耐盐植物在细胞水平上也具有耐盐性。从甘蔗和烟草中选择的盐耐受性（ST）细胞对盐条件显示出不同的反应。在NaCl培养基上培养的甘蔗ST细胞维持K，Mg和Ca含量。另一方面，烟草细胞在高盐状态下显示出K，Mg和Ca的含量较低，但增长良好。这些表明两个ST细胞具有不同的盐耐受机制。随着渗透调节剂的甘蔗积聚，烟草积聚了甜菜碱，但这些含量低于盐植物细胞。对培根的制剂和培养进行了测试，目的是为了引入热带作物的耐盐能力。来自几个盐生植物的培养细胞被处理不同的酶组合。裤子激素的组合和细胞增殖能力会影响干净的原生质体。将阿特里普物种的原生质体应用于与烟草叶叶叶原生质体的原生质体融合。