Molecular basis of plant viral virlence and cross-protection ability

植物病毒毒力和交叉保护能力的分子基础

基本信息

批准号：
62480043
负责人：
OKADA Yoshimi
金额：
$ 4.29万
依托单位：
University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

Attenuated strains of tobacco mosaic virus (TMV) have been used to protect crops against virulent strains. To determine how the genes of attenuated strain participates in the behaviour of multiplication of TMV, we compared the synthesis of viral-coded protein in tobacco protoplast. It was revealed that the mutations, which are responsible for the viral attenuation caused a reduction of the synthesis of viral-coded 30K protein with a cell-to-cell movement function.A full-length cDNA copy of TMV genomic RNA was constructed and introduced into the genomic DNA of tobacco plants using a disarmed ti plasmid vector. Transformed plants showed typical symptoms of TMV infection. This is the first example of the expression of RNA virus genomic RNA in planta.Using this eyetem, we introduced a cDNA copy of a mild strain of TMV into tobacco plants. The transgenic plants did not develop any severe symptoms upon inoculation with a virlent TMV strain, indicating that these transgenic plants were cross protected against TMV infection.The system described can be a useful model system to study the mechanism of cross protection.

烟草花叶病毒（TMV）的减毒株已被用来保护农作物免受有毒株的侵害。为了确定减毒株的基因如何参与TMV的增殖行为，我们比较了烟草原生质体中病毒编码蛋白的合成。结果表明，导致病毒减毒的突变导致病毒编码的具有细胞间移动功能的 30K 蛋白的合成减少。构建并引入了 TMV 基因组 RNA 的全长 cDNA 拷贝使用解除武装的 ti 质粒载体将其插入烟草植物的基因组 DNA 中。转化植物表现出TMV感染的典型症状。这是RNA病毒基因组RNA在植物中表达的第一个例子。使用该eyetem，我们将TMV温和毒株的cDNA拷贝引入烟草植物中。转基因植物在接种无毒TMV毒株后没有出现任何严重症状，表明这些转基因植物受到交叉保护以抵抗TMV感染。所描述的系统可以是研究交叉保护机制的有用模型系统。