Immunogenetic studies on the resistamce of chickens to Marek's disease

鸡抗马立克氏病的免疫遗传学研究

基本信息

批准号：
61480078
负责人：
OKADA Ikuo
金额：
$ 3.71万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1988
项目状态：
已结题

项目摘要

Four pairs of chicken lines selected for high (H) and low (L) competences in graft-versus-host reaction (GVHR), serum immunoglobulin g level (IgG), immune response to rabbit serum albumin (RSA) and anaphylaxy shock to bovine serum albumin (BSA) were examined for their genetic resistances to Marek's disease (MD) and to transplantable MD lymphoma cell line MSB1-41C (41C).1. When GVHR-H and L lines were inoculated with Md virus, their relative ranking of Md resistance varied by experiments. However, the resistance to 41C was constantly higher in the H line than in the l line.2. In all pairs of IgG-, RSA- and BSA-selected H and L lines, the resistance to MD virus was lower in the H lines than in the respective L lines, whereas the 41C resistance was higher in the H line than in the respective L line.3. Chicks of GVHR-H and L lines were thymectomized (TX) and part of the TX chicks received thymus transplants from the partner's line (TX-PT). The resistance of TX-PT group which received thymus transplants from the susceptible line was more susceptible to both MD virus and 41C than the TX group of the same line. On the other hand, the TX-PT group received resistant line's thymus showed almost the same resistance with the TX group of the same line.4. Reciprocal transplantations of spleen cells were carried out between GVHR-H and L lines. Increase of resistance to 41C was observed in the H line received L line's spleen cells, whereas decrease was observed in the L line received H line's cells.5. Cell growth factor 105Kgp was found in serum of chickens inoculated with 41C. No difference in 105Kgp level was found between the GVHR-H and L lines. It seemed that there was no relationship between 105Kgp level and MD resistance.

在移植物抗宿主反应（GVHR）中选择高（H）和低（L）能力的四对鸡线，血清免疫球蛋白G水平（IgG），对兔血清白蛋白（RSA）的免疫反应和对牛血清白蛋白（BSA）的过敏反应（rsa）和过敏反应的疾病及其属于遗传的疾病（Marek）的疾病（Marek）的疾病。线MSB1-41C（41C）.1。当GVHR-H和L线与MD病毒接种时，其MD电阻的相对排名通过实验而变化。但是，在H线中，对41C的电阻不断高于L线。2。在所有IgG-，RSA和BSA选择的H和L系中，H线中对MD病毒的抗性低于相应的L线，而H线中的41C电阻在相应的L系中高于相应的L线。3。将GVHR-H和L线的小鸡进行胸膜切除术（TX），TX雏鸡的一部分接受了伴侣线（TX-PT）的胸腺移植。从易感线接收百里香移植的TX-PT组的耐药性比同一线的TX组更容易受到MD病毒和41C的影响。另一方面，TX-PT组接受了抗性线的胸腺，与同一线的TX组显示出几乎相同的电阻。4。 GVHR-H和L线之间进行了脾细胞的相互移植。在接收到L线的脾细胞的H系中观察到对41C的电阻增加，而在接收到H系的L系的L系中观察到降低。5。在接种41c的鸡的血清中发现细胞生长因子105kgp。 GVHR-H和L线之间没有发现105公斤水平的差异。似乎105公斤水平与MD阻力之间没有关系。