A NEW SEPARATION MECHANISM OF CENTRIFUGAL PARTITION CHROMATOGRAPHY ASSOCIATED WITH LIQUID MEMBRANE TRANSPORT SYSTEM.

液膜传输系统离心分配色谱新分离机制。

基本信息

批准号：
61470026
负责人：
ARAKI Takeo
金额：
$ 4.48万
依托单位：
Shimane University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1988
项目状态：
已结题

项目摘要

The investigators have found that when a separator is added to the Centifugal Partition Chromatography (CPC), its separation mechanism becomes to be similar to that of liquid membrane transport (LMT) systems. The main research project of this fund was concentrated on the extension of this new mechanism to the problems of separation/concentration/isolation of materials, and to clarify the relation between the "separator-aided CPC" and LMT systems. The apparatus employed was Sanki Engi-neering CPC-L.L. (Model NMF).In considering the mechanistic similarity and difference of these two techniques, a correla-tion guideline table was constructed. Direct comparison between the CPC and LMT using nitrophenols (2,4,6-trinitrophenol and p-nitro-phenol) as aqueous sample solution, and n-butanol as separator in chloroform solvent. In both technique stronger interaction with n-butanol was observed for p-nitro-phenolthan for 2,4,6-trinitrophenol. NMR spectroscopically pure fractions could readily be o … More btained by CPC from the equimolar mixture sample. At the same time, selective LMT was attained using simple and costless "carrier", i.e.. n-butanol. Hence, the CPC can be regarded as a new type of multistage LMT system. Similar relation between separator-aided CPC and LMT was observed for rare earth metal ion (RE^<3+>) samples when Di-(2-ethylhexyl)phosphoric acid (D2EHPA), a familiar extractant of these ions, was used as separator. The interaction of the RE^<2+> ions with D2EHPA in CPC and LMT was found to obey a simple equilibrium known in the corresponding extraction processes. then, by adjusting the factors which affect the equilibrium, CPC separation of righter RE^<3+> ions was attained relatively readily. Also, selective LMT of the various sets of RE^<3+> ions was realized by the use of D2EHPA. Since the separation mechanism involved in these cases is essentially acid-base type interaction. The peak broadening in CPC became significant with increase of the RE's atomic number, especially for heavier RE^<3+> ions. However, the relation between CPC and LMT was retained throughout. Further extension was attempted to opitical resolution in CPC, and fundamental pre-requisites for effective resolution by CPC wre tabulated. We are now continuing trying experimentally along this pre-requi-sites. Thus, the investigators were capable of showing high utillity of the separator-aided CPC and the applicavility of these CPC data to costless sensing procedures using liquid membranes. Less

研究人员发现，当将分离器添加到centifugal分区色谱（CPC）中时，其分离机制变得类似于液体膜转运（LMT）系统的分离机制。该基金的主要研究项目集中在将这种新机制扩展到材料的分离/浓度/隔离问题上，并阐明“分离器辅助CPC”和LMT系统之间的关系。该设备进行了Sanki Engieering CPC-L.L。（NMF模型）。考虑到这两种技术的机械相似性和差异，构建了相关指南表。使用亚硝基苯酚（2,4,6-三硝基苯酚和P-硝基苯酚）作为水样溶液，以及N-丁二醇作为氯仿溶液中的N-丁醇，使用CPC和LMT进行直接比较。在这两种技术中，对于2,4,6-三硝基苯酚，观察到与N-丁醇的相互作用更强。 NMR光谱上的纯级分很容易成为……从等效混合物样品中CPC更容易。同时，使用简单且无价的“载体”（即N-丁醇）附加了选择性LMT。因此，CPC可以被视为一种新型的多阶段LMT系统。当稀土金属离子（Re^<3+>）样品中，分离剂辅助的CPC和LMT之间的关系类似，当Di-（2-乙基己基）磷酸（D2EHPA）（这些离子的熟悉提取物）用作分离剂时。发现Re^<2+>离子与CPC和LMT中D2EHPA的相互作用遵守在相应的提取过程中已知的简单等价br。然后，通过调整影响等效繁殖的因素，相对容易地获得了righter re^<3+>离子的CPC分离。同样，通过使用D2EHPA实现了各种RE^<3+离子集的选择性LMT。由于在这些情况下涉及的分离机制基本上是酸碱类型的相互作用。随着RE原子数的增加，CPC的峰值扩大变得显着，尤其是对于较重的Re^<3+>离子而言。但是，CPC和LMT之间的关系始终保留。试图进一步扩展以选择CPC中的政治分辨率，以及通过CPC WRE制定的有效分辨率的基本先决条件。现在，我们正在继续沿着这一先决条件进行实验尝试。这就是研究人员能够显示出分离剂辅助CPC的高效用，以及这些CPC数据使用液体膜上的无需成本敏感性程序的适用性。较少的