The role of neuropeptides on ocular physiology and pathophysiology

神经肽对眼生理学和病理生理学的作用

基本信息

批准号：
60480389
负责人：
FUKUDA Masakatsu
金额：
$ 2.94万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480389/
关键词：
Herdetic keratitis Substance P Intraocular pressure Vasoactive intestinal peptide Neuropeptide Y ERG

项目摘要

1. We investigated the sequential changes of substance P (SP), one of the neurotransmitter candidates of ocular sensory nerves, in acute herpetic keratitis of mice using indirect immunofluorescence method and radioimmunoassey (RIA). In cornea, the SP immunoreactivity was slightly reduced only at herpetic focus on the 2nd day post-infection (PI), and no SP positive fibers were visualized in whole cornea on 4th day PI. In trigeminal ganglia (TG), however, no change was observed in SP immunoreactive nerve cells and fibers even after infection. RIA studies revealed that the amount of SP in cornea was dramatically reduced on 4th day PI, and that TG of infected mice contained almost as much amount of SP as those of controls did at the time examined.2. We also investigated the effects of neuropeptides on ocular circulation of rabbit. Vasoactive intestinal polypeptide (VIP), when injected i.v., increased the blood volume in the eye (BV) and intraocular pressure (IOP), and decreased systemic blood pressure (BP). When NPY was injected i.v., the reduction of BV and IOP, and the rise of BP were observed. SP had no effect on any of those parameters. These results indicate the potential usefulness of NPY on glaucoma therapy.3. Although many neuropeptides have been immunohistochemically identified in retina, their physiological roles are still unclear. We evaluated how those neuropeptides might affect ERG of chick retina, in vivo at various stages of development. The alternation of oscillatory potentials ( OP1 and OP2 ) were observed after intracameral injection of VIP especially at early stages after hatching, while no effect on oscillatory potentials was detected after injection of any other peptides tested. These observation suggests that VIP has modulatory effects on the synaptic circuits in the retina particularly during developments.

1.我们采用间接免疫荧光法和放射免疫分析（RIA）研究了眼感觉神经候选神经递质之一P物质（SP）在小鼠急性疱疹性角膜炎中的连续变化。在角膜中，仅在感染后第2天（PI）的疱疹病灶处SP免疫反应性略有降低，并且在感染后第4天整个角膜中未观察到SP阳性纤维。然而，在三叉神经节（TG）中，即使在感染后，SP 免疫反应性神经细胞和纤维也没有观察到变化。 RIA研究表明，感染后第4天，角膜中的SP含量显着减少，并且感染小鼠的TG中的SP含量几乎与对照组小鼠在检查时的含量相同。 2．我们还研究了神经肽对家兔眼循环的影响。静脉注射血管活性肠多肽（VIP）时，可增加眼部血容量（BV）和眼内压（IOP），并降低全身血压（BP）。当静脉注射 NPY 时，观察到 BV 和 IOP 降低，血压升高。 SP 对任何这些参数都没有影响。这些结果表明NPY在青光眼治疗中的潜在用途。3．尽管许多神经肽已在视网膜中被免疫组织化学鉴定，但它们的生理作用仍不清楚。我们评估了这些神经肽如何影响处于不同发育阶段的体内小鸡视网膜的 ERG。前房注射 VIP 后观察到振荡电位（OP1 和 OP2）的交替，特别是在孵化后的早期阶段，而注射任何其他测试肽后未检测到对振荡电位的影响。这些观察结果表明，VIP 对视网膜突触回路具有调节作用，特别是在发育过程中。