The regulatory mechanism of sclerotium differentiation in Rhizoctonia solani

立枯丝核菌菌核分化的调控机制

• 批准号: 60480001
• 负责人: ISHIKAWA Tatsuo
• 金额: $ 4.1万
• 依托单位: Institute of Applied Microbiology, University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480001/
• 关键词: Rhizoctonia solani; Sclerotium; Differentiation; プラスミド

1. The Basidiomycete Rhizoctonia solani strains which carry the linear DNA plasmids showed slow growth and weak pathogenicity. The correlationship has been observed between the production of oxalic acid and sclerotium formation. Mycelial growth and sclerotium formation were coordinately repressed in low pH media and stimulated by adjusting pH at 5.5. Thus it has been concluded that the inability of sclerotium formation observed in the strains carrying plasmids is resulted from low pH of media caused by the production of oxalic acid.2. The plasmids found in this fungus were linear DNA. Two plasmids, pRS64-1 and pRS64-2 were identified by compairing the restriction maps. The restriction maps of these plasmids were different in several restriction sites, but both plasmids carried homologous DNA sequences hybridized each other.3. Genomic DNA of R. solani digested with a restriction enzyme Pst I was hybridized with the labeled plasmid fragments. Approximately 13 kilobases fragment of genomic DNA has a homologous sequence with the plasmid DNA. The results suggest that the plasmid DNA may be integrated into the genomic DNA.

1、携带线性DNA质粒的担子菌立枯丝核菌生长缓慢，致病力弱。已经观察到草酸的产生和菌核形成之间的相关性。菌丝体生长和菌核形成在低 pH 培养基中协同抑制，并通过将 pH 值调节至 5.5 来刺激。由此推断，携带质粒的菌株不能形成菌核是由于草酸的产生导致培养基pH值过低造成的。 2．在这种真菌中发现的质粒是线性DNA。通过比较限制性图谱鉴定出两个质粒pRS64-1和pRS64-2。这些质粒的酶切图谱在几个酶切位点上有所不同，但两个质粒都携带同源DNA序列，彼此杂交。 3．将用限制酶 Pst I 消化的立枯丝核菌的基因组 DNA 与标记的质粒片段杂交。基因组DNA的大约13kb片段与质粒DNA具有同源序列。结果表明质粒DNA可能整合到基因组DNA中。

项目成果

Yoh-ichi Shinma and Tatsuo Ishikawa: "Characterization of DNA plasmids in Rhizoctonia solani"

Yoh-ichi Shinma 和 Tatsuo Ishikawa：“立枯丝核菌 DNA 质粒的表征”