A pilot study of new adhesion system of composite resion to dentin

新型复合树脂牙本质粘合体系的初步研究

基本信息

批准号：
05671597
负责人：
SATOU Naoki
金额：
$ 1.41万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671597/
关键词：
Tannic acid Dentin collagen Oral streptococci Bonding agent 象牙質前処理剤

项目摘要

This in vitro study examined the effect of concentration and/or incubation time of tannic acid to dentin and bacteria. The results showed that tannic acid inhibited the dissolution of dentin collagen treated with tripsin and phosphoric acid, depending on the increasing of concentration and incubation time of tannic acid. SEM study also revealed tannnic acid protected the collagen structure treated with tripsin and phosphoric acid. Minimal inhibitory concentration of tannic acid to S.mutans was 300 mug/ml, while that of another streptococci was 400mug/ml. Phenol coefficient of tannic acid to S.mutans OMZ 175 was 0.6.these results indicate that antimicrobial action of tannic acid give play to strain such as S.mutans. Strerillizing power of power of 300 mug/ml tannic acid with S.mutans OMZ 175 for 15 min incubation times was the highest among the strains which were haevested from stationally phase, survival rate of S.mutans OMZ 175 decreased to 2% of control. As the results of their being coated and/or uncoated saliva, the surface hydrophobicity of all restoratives and teeth decreased and their negative surface charge increased when these surfaces were treated with tannnic acid. The number of adherent streptococci decreased under above conditions. Antimicribial action of bonding agent including tannic acid showed higher than that of bonding agent without tannic acid. However the specimens without tannic acid which were not cured or were cred and immediately used investigation in early time also showed antimicribial action. Bonding agent including tannic acid exhibited high adaptation to enamel and dentin surfaces. As suggested these results that tannic acid improve anti-enzyme property of dentin and antimicrob ial action, the existence of tannic acid between tooth and restorative surfacies is useful for inhibitation of secondory caries.

这项体外研究检查了单宁酸对牙本质和细菌的浓度和/或孵育时间的影响。结果表明，单宁酸抑制了用三肽和磷酸处理的牙本质胶原蛋白的溶解，这取决于单宁酸的浓度和孵育时间的增加。 SEM研究还揭示了单胞酸保护用tripsin和磷酸处理的胶原蛋白结构。单宁酸抑制浓度最小为S.Mutans为300毫克/mL，而另一链球菌的抑制剂浓度为400mug/ml。单宁酸对S.Mutans OMZ 175的苯酚系数为0.6。这些结果表明，单宁酸的抗菌作用使S.Mutans等菌株发挥作用。 300杯/ml单宁与S.Mutans OMZ 175的功率功率15分钟的孵育时间是菌株中最高的菌株，这些菌株是从固定相位，S.Mutans OMZ 175的存活率降低到对照的2％。随着它们被覆盖和/或未涂层的唾液的结果，所有恢复和牙齿的表面疏水性降低了，当用单胞酸处理这些表面时，其负表面电荷增加。在上述条件下，粘附链球菌的数量减少。包括单宁酸在内的键合剂的抗微生物作用高于无单宁酸的粘合剂。然而，没有单宁酸的标本未固化或被信用，并在早期立即使用研究也显示出抗毛酸作用。包括单宁酸在内的粘合剂表现出对搪瓷和牙本质表面的高适应性。正如这些结果表明，单宁酸改善了牙本质和抗微生物作用的抗酶特性，牙齿和恢复性表面之间存在单宁酸对抑制二含症状是有用的。