A pilot study of new adhesion system of composite resion to dentin

新型复合树脂牙本质粘合体系的初步研究

基本信息

批准号：
05671597
负责人：
SATOU Naoki
金额：
$ 1.41万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671597/
关键词：
Tannic acid Dentin collagen Oral streptococci Bonding agent 象牙質前処理剤

项目摘要

This in vitro study examined the effect of concentration and/or incubation time of tannic acid to dentin and bacteria. The results showed that tannic acid inhibited the dissolution of dentin collagen treated with tripsin and phosphoric acid, depending on the increasing of concentration and incubation time of tannic acid. SEM study also revealed tannnic acid protected the collagen structure treated with tripsin and phosphoric acid. Minimal inhibitory concentration of tannic acid to S.mutans was 300 mug/ml, while that of another streptococci was 400mug/ml. Phenol coefficient of tannic acid to S.mutans OMZ 175 was 0.6.these results indicate that antimicrobial action of tannic acid give play to strain such as S.mutans. Strerillizing power of power of 300 mug/ml tannic acid with S.mutans OMZ 175 for 15 min incubation times was the highest among the strains which were haevested from stationally phase, survival rate of S.mutans OMZ 175 decreased to 2% of control. As the results of their being coated and/or uncoated saliva, the surface hydrophobicity of all restoratives and teeth decreased and their negative surface charge increased when these surfaces were treated with tannnic acid. The number of adherent streptococci decreased under above conditions. Antimicribial action of bonding agent including tannic acid showed higher than that of bonding agent without tannic acid. However the specimens without tannic acid which were not cured or were cred and immediately used investigation in early time also showed antimicribial action. Bonding agent including tannic acid exhibited high adaptation to enamel and dentin surfaces. As suggested these results that tannic acid improve anti-enzyme property of dentin and antimicrob ial action, the existence of tannic acid between tooth and restorative surfacies is useful for inhibitation of secondory caries.

这项体外研究检查了单宁酸的浓度和/或孵育时间对牙本质和细菌的影响。结果表明，单宁酸对胰蛋白酶和磷酸处理后的牙本质胶原蛋白的溶解有抑制作用，其抑制作用与单宁酸浓度和孵育时间的增加有关。 SEM 研究还表明，单宁酸可以保护用胰蛋白酶和磷酸处理的胶原蛋白结构。单宁酸对变形链球菌的最低抑菌浓度为300μg/ml，而对另一种链球菌的最低抑菌浓度为400μg/ml。单宁酸对变形链球菌OMZ 175的苯酚系数为0.6。这些结果表明单宁酸对变形链球菌等菌株发挥抗菌作用。 300μg/ml单宁酸功率与S.mutans OMZ 175孵育15 min的杀菌力是静止期收获的菌株中最高的，S.mutans OMZ 175的存活率下降至对照的2％。由于涂覆和/或未涂覆唾液，所有修复体和牙齿的表面疏水性降低，并且当用单宁酸处理这些表面时，它们的负表面电荷增加。在上述条件下，粘附链球菌的数量减少。含有单宁酸的粘合剂的抗菌作用表现出比不含单宁酸的粘合剂更高。然而，未固化或早期鉴定并立即使用的不含单宁酸的标本也显示出抗菌作用。包含单宁酸的粘合剂对牙釉质和牙本质表面表现出高度的适应性。正如单宁酸改善牙本质的抗酶特性和抗菌作用的这些结果所表明的，牙齿和修复体表面之间存在单宁酸对于抑制继生龋齿是有用的。