Detection of periodontal disease activity by T cell receptor repertoire and cytokine profile of infiltrating T cells

通过 T 细胞受体库和浸润 T 细胞的细胞因子谱检测牙周病活动

基本信息

批准号：
05671593
负责人：
YAMAZAKI Kazuhisa
金额：
$ 1.41万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671593/
关键词：
Periodontitis Gingivitis Functional subsets Th1 Th2 T cell receptor Immunohistochemistry IL-4 IL-6

项目摘要

In order to characterize the T cells infiltrating gingival tissues with periodontitis, T cell receptor repertoire and cytokine profile were investigated by means of immunohistological procedure. Periodontitis tissues were obtained at the time of periodontal surgery after completion of initial therapy. Gingivitis tissues as a control were also obtained from non-periodontitis lesions. Serial cryostat sections from each specimen were immunostained for TCR V gene products (Valpha2, Vbeta5.2-3, Vbeta5.3, Vbeta5.1, Vbeta6.7, Vbeta8, Vbeta12) and cytokines (IL-2, IFN-gamma, IL-4, IL-6) with alkaline phosphataseanti alkaline phosphatase method. Relative percentages were caluclated by dividing the number of each positive cell by total number of CD3 positive cells. Moreover, for comparison between gingival tissues and circulating pool, peripheral blood mononuclear cells from both groups were analyzed for the TCR repertoire by flow cytometry. The pattern of each TCR V gene product expression in i … More nflamed gingiva exhibited individual variation, nevertheless a consistent pattern emerged. The Vbeta5 subfamily and Vbeta6.7 were frequently used repertoires in gingiva, whereas the Valpha2 and Vbeta8 subfamily were underexpressed in most cases. Furthermore, the TCR V gene product expression in gingival tissue was biased compared with autologous peripheral blood. Three out of 10 periodontitis subjects showed 1 or 2 strikingly overrepresented repertoire comparatively with autologous blood. In these 3 subjects Vbeta6.7 was overexpressed in two cases and Vbeta5.2-3, Vbeta8 and Vbeta12 were overexpressed in one case. With respect to the cytokine profile, the proportion of IL-4- and IL-6- producing T cells was higher in periodontitis than in gingivitis. Moreover, a siginificant positive correlation between the proportion of IL-4-producing cells and the B cell : T cell ratio was noted. These data suggest that gingival T cells are not randomly mobilized from peripheral blood and that local events influence the TCR repertoire at the level of T cell recruitment or T cell expansion and the functional subsets of those T cells are Th2 type. Less

为了表征牙周炎浸润牙龈组织的T细胞，通过免疫组织学程序研究了T细胞受体库和细胞因子谱。初始治疗完成后，在牙周手术时获得牙周炎组织。牙龈炎组织还从非周期性炎病变中获得。对TCR V基因产物（valpha2，vbeta5.2-3，vbeta5.3，vbeta5.1，vbeta5.1，vbeta6.7，vbeta8，vbeta8，vbeta12）和细胞因子（IL-2，ifn-2，ifn-gamma，ifn-gamma groundi flochiation）（IL-2，IL-4-6），对每个样品的串行低温抑制切片进行免疫染色（valpha2，vbeta5.2-3，vbeta5.2-3，vbeta5.3，vbeta5.3，vbeta5.3，vbeta5.1，vbeta6.7，vbeta12）方法。通过将每个正细胞的数量除以CD3阳性细胞的总数，从而将相对百分比归类。此外，为了比较牙龈组织和循环池，通过流式细胞仪分析了两组的外周血单核细胞的TCR库。 I…中每个TCR V基因产物表达的模式暴露了个体变异，但出现了一致的模式。 VBETA5亚家族和VBETA6.7经常在gingiva中使用，而在大多数情况下，valpha2和valpha2和vbeta8亚家族都不被渗透。此外，与自体外周血相比，牙龈中的TCR V基因产物产物表达有偏见。在10个牙周炎受试者中，有3名与自体血相比表现出1或2个惊人的过分代表性曲目。在这三个受试者中，VBETA6.7在两种情况下过表达，VBETA5.2-3，VBETA8和VBETA12在一个情况下过表达。关于细胞因子的特征，牙周炎的IL-4-和IL-6-产生T细胞的比例高于牙龈炎。此外，注意到IL-4产生细胞的比例与B细胞：T细胞比率之间存在毫无用处的阳性相关性。这些数据表明，牙龈T细胞不是从外围血液中随机动员的，并且局部事件在T细胞募集或T细胞膨胀水平上影响TCR库，这些T细胞的功能子集是Th2类型的。较少的