Expression of human IL-2 receptor beta and gamma chains in mucosal immue lymphatic tissue and the immunopathological meaning.

人IL-2受体β链和γ链在粘膜免疫淋巴组织中的表达及其免疫病理学意义

• 批准号: 05670160
• 负责人: GOTO Kunihiko
• 金额: $ 1.28万
• 依托单位: Tohoku University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670160/
• 关键词: Interleukin-2 (IL-2); IL-2 receptor (IL-2R); IL-2Rbeta chain; IL-2Rgamma chain; mucosal imunune lymphatic tissue; colon cancer; Crohn's disease; Ulcerative colitis; 腫瘍性大腸炎; インターロイキン2; インターロイキン2レセプター; IL-2レセプターβ鎖; 粘膜免疫; 免疫組織化学; 免疫電顕; IL-2レセプ

It is an important urgentnecessity to clarity the signaltransduction of growth factors which make division of or proliferate target cells or tumorcells.We elarified in the signal transduction of IL-2 receptor beta chans that ;1) The expression of IL-2Rbeta was located in surface membrane, 2) The number of IL-2Rbeta was 1/20 of IL-2Ralpha by immuno-gold technique, 3) 5 minutes after the treatment of IL-2Rbeta, the internalization of IL-2Rbeta was a peak, and 4) 30 minutes after the treatment, IL-2Rbeta was localized at phinuclear space. These results confirm biochemical those that IL-2beta participates in the prdiferative of cells.Furtheremore, Sugamura et al. of our university prepared anti-IL-2Rgamma. Now, we examined the localization of anti-IL-2Rgamma in cell lines of MT-2, MT-1, and ILT-Mat. We detected the same localization of anti-IL-2Rgamma in those cells as served in IL-2Rbeta.We are in the way of detecting the anti-IL-2Rbeta localization in human gastrointestinal mucosa, their tumors, colen cancers, and colons of Cromn's disease and Uleerative colitis.

这是清晰的生长因子的信号转移，这些因素的信号转移，这些因素使靶细胞或肿瘤分裂或增殖。我们在IL-2受体β元的信号转导中所阐明； 1）IL-2RBETA的表达在表面膜中的表达，2）IL-2RBETA的数量是IL-2RBETA的数量，是IL-2RBETA的数量3分钟。 IL-2rbeta，IL-2rbeta的内在化是峰值，4）处理后4）30分钟，IL-2RBETA位于纤维化空间。这些结果证实了IL-2Beta参与细胞的杂种的生化。我们的大学准备了Anti-IL-2rgamma。现在，我们检查了抗IL-2rgamma在MT-2，MT-1和ILT-MAT​​的细胞系中的定位。我们在这些细胞中检测到与IL-2RBETA中的抗IL-2rgamma相同的定位。我们正在检测人类胃肠道粘膜中的抗IL-2RBETA定位，其肿瘤，Colen Colen Cancers和Cromn的Cromn疾病和Ureeratival Colerative Colielic Colias Is。

项目成果

Goto: "Hydrophobic accessible surface areas are proportional to binding energies of serine protease-protein inhibitor complexes." Biochem.Biophys.Res.Commun.206. 497-501 (1995)

Goto：“疏水性可及表面积与丝氨酸蛋白酶-蛋白质抑制剂复合物的结合能成正比。”

Goto,K.: "Hydrophobic accessible surface areas are proportional to binding energies of serine protease-protein inhibitor complexes" Biochemical and Biophysical Research Communications. 206(2). 497-501 (1995)

Goto,K.：“疏水性可及表面积与丝氨酸蛋白酶-蛋白质抑制剂复合物的结合能成正比”生物化学和生物物理研究通讯。

Goto,K.: "Do α-helix slidings occur during bacteriorhodopsin structureproton pump-photocycle-inergetics coupling." Biochemical and Biophysical Research Communication. (in press). (1995)

Goto, K.：“细菌视紫红质结构质子泵-光循环-惰性耦合过程中是否会发生 α-螺旋滑动。”生物化学和生物物理研究通讯（1995 年出版）。

Goto,K.: "The-on off mechanisms of the nicotinic acetylcholine receptor jon channel are performed by thermodynamic forces." Journal of Theoretical Biology. 170. 267-272 (1994)

Goto,K.：“烟碱乙酰胆碱受体 jon 通道的开关机制是由热力学力执行的。”

Goto,K.: "Hydrophobic accessible surtace areas are proportional to binding energies of serine protease-protein inhibitor complexes" Biochemical and Biophysical Research Communications. 206. 497-501 (1995)

Goto,K.：“疏水性可及表面面积与丝氨酸蛋白酶-蛋白质抑制剂复合物的结合能成正比”生物化学和生物物理研究通讯。